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蛙胚眼预定水晶体诱导脑壁形成次生眼杯的实验研究
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作者 李新人 余启祥 +1 位作者 李桃生 王希成 《解剖学报》 CAS 1987年第4期443-446,468,共5页
本实验以15~16期的黑斑蛙胚胎为材料,采用切除眼泡,或切除眼泡后填补一块前脑侧壁组织,或将眼泡连同部分前脑侧壁组织一并切下并旋转180度等显微手术,使预定水晶体外胚层与前脑侧壁组织接触。结果表明,前脑侧壁接触了预定水晶体以后,由... 本实验以15~16期的黑斑蛙胚胎为材料,采用切除眼泡,或切除眼泡后填补一块前脑侧壁组织,或将眼泡连同部分前脑侧壁组织一并切下并旋转180度等显微手术,使预定水晶体外胚层与前脑侧壁组织接触。结果表明,前脑侧壁接触了预定水晶体以后,由于受到后者的诱导作用,接触部位的脑壁组织能转化为次生眼杯。在282个手术例中,计有81例出现次生眼杯,成功率达28.8%。本文对次生眼杯的成因及某些规律进行了讨论,并对负反应的某些原因作了分析。 展开更多
关键词 次生眼杯形成 预定水晶体 诱导 蛙胚
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蛙胚眼间质细胞对色素上皮分化前后的影响
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作者 李新人 余启祥 王希成 《解剖学报》 CAS 1987年第4期439-442,468,共5页
本实验以泽蛙胚作材料。色素上皮分化前或分化后的眼泡经刮除间质细胞后,移植于同种蝌蚪体腔中培养,观察间质细胞对色素上皮分化前后的影响。结果证明,色素上皮分化前刮除间质细胞时,色素上皮不能发育或仅有片段色素上皮形成;而色素上... 本实验以泽蛙胚作材料。色素上皮分化前或分化后的眼泡经刮除间质细胞后,移植于同种蝌蚪体腔中培养,观察间质细胞对色素上皮分化前后的影响。结果证明,色素上皮分化前刮除间质细胞时,色素上皮不能发育或仅有片段色素上皮形成;而色素上皮开始分化或已经分化后刮除间质细胞时,色素上皮虽仍能发育和存在,但均脱离正常位置。这提示,间质细胞对眼色素上皮分化前后均有重要影响。而其作用性质似乎又有不同:在分化前,间质细胞的存在是色素上皮正常分化所必需的条件;在分化后,似乎起维持正常位置的机械作用。 展开更多
关键词 色素上皮 间质细胞 眼色素上皮分化 蛙胚
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Embryonic development of the concave-eared torrent frog with its significance on taxonomy 被引量:5
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作者 熊荣川 江建平 +2 位作者 费梁 王斌 叶昌媛 《Zoological Research》 CAS CSCD 北大核心 2010年第5期490-498,共9页
We investigated the early embryonic and larval development of the concave-eared torrent frogs, Odorrana tormota (Amphibia, Anura, Ranidae). Embryos were derived from artificial fertilization of frogs’ eggs, and the... We investigated the early embryonic and larval development of the concave-eared torrent frogs, Odorrana tormota (Amphibia, Anura, Ranidae). Embryos were derived from artificial fertilization of frogs’ eggs, and the staging of development was based on morphological and physiological characteristics. Two major periods of development were designated: i) early embryonic period, from fertilization to operculum completion stage, lasted for 324 h at water temperature (WT) 18 ?23℃; ii) larval period, from operculum completion stage to tail absorbed stage, took 1207 h at WT 20 ? 24℃. Tadpoles of the concave-eared torrent frogs showed no evidence of abdominal sucker. Absence of this key characteristic supports the view from molecular systematics that concave-eared torrent frog does not belong to the genus Amolops. Two cleavage patterns were observed in embryos at 8-cell and 16-cell stages, with Pattern I2 (latitudinal cleavage at the 8-cell stage, and meridional cleavage at the 16-cell stage with two perpendicular meridional furrows) being the predominant pattern and only 1.5% belonging to Pattern II (meridional cleavage at the 8-cell stage and latitudinal cleavage at the 16-cell stage). The factors affecting cleavage and hatching ratios, developmental speed, and ecological adaptation were discussed. 展开更多
关键词 Odorrana tormota Embryonic development Artificial fertilization Abdominal sucker Embryonic cleavage
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Ni^(2+) treatment causes cement gland formation in ectoderm explants of Xenopus laevis embryo
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作者 HUANGYONG XIAOYANDING 《Cell Research》 SCIE CAS CSCD 1999年第1期71-76,共6页
We found T-type calcium channel blocker Ni2+ can efficiently induce the formation of cement gland in Xenopus laevis animal cap explants. Another T-type specific calcium channel blocker Amiloride can also induce the fo... We found T-type calcium channel blocker Ni2+ can efficiently induce the formation of cement gland in Xenopus laevis animal cap explants. Another T-type specific calcium channel blocker Amiloride can also induce the formation of cement gland, while L-type specific calcium channel blocker Nifedipine has no inductive effect. These results may offer us an new approach to study the differentiation of cement gland through the change of intracelluax calcium concentration. 展开更多
关键词 Ni^(2+) cement gland GASTRULATION T-type calcium channel
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