Bioassay of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab.The results showed that 1%VBL,12.5 μL·L-1 Chlorbenz...Bioassay of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab.The results showed that 1%VBL,12.5 μL·L-1 Chlorbenzuron,15 μL·L-1 Trichlorphon had better synergistic effect.Spraying of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon and PoNPV to control 2～3 instar larvae of second generation in field,the concentrations of PoNPV were 8×106 PIB·mL-1,4×106 PIB·mL-1,2×106 PIB·mL-1,1×106 PIB·mL-1,5×105 PIB·mL-1,there were 20 treatments.It was indicated that when the concentration was the same,the effect of PoNPV added synergists was better than that without synergists.展开更多
The gene library of Parocneria orienta nuclear polyhedrovirus(PaorNPV)was partly constructed by cloing some fragments of BamH I and EcoR I of PaorNPV genomic DNA into pGEM3Z(f-)as a vector.Using the chitinase gene of ...The gene library of Parocneria orienta nuclear polyhedrovirus(PaorNPV)was partly constructed by cloing some fragments of BamH I and EcoR I of PaorNPV genomic DNA into pGEM3Z(f-)as a vector.Using the chitinase gene of HearSNPV probed with[α- 32 P]dCTP,the chitinase gene of PaorNPV was localized on the fragments of BamH I-D,EcoR I-A,Hind Ⅲ-A,Pst I-D and Xho I-G/H respectively.At the same time,the fragment of BamH I-D containing the chiA has been cloned.展开更多
文摘Bioassay of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab.The results showed that 1%VBL,12.5 μL·L-1 Chlorbenzuron,15 μL·L-1 Trichlorphon had better synergistic effect.Spraying of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon and PoNPV to control 2～3 instar larvae of second generation in field,the concentrations of PoNPV were 8×106 PIB·mL-1,4×106 PIB·mL-1,2×106 PIB·mL-1,1×106 PIB·mL-1,5×105 PIB·mL-1,there were 20 treatments.It was indicated that when the concentration was the same,the effect of PoNPV added synergists was better than that without synergists.
文摘The gene library of Parocneria orienta nuclear polyhedrovirus(PaorNPV)was partly constructed by cloing some fragments of BamH I and EcoR I of PaorNPV genomic DNA into pGEM3Z(f-)as a vector.Using the chitinase gene of HearSNPV probed with[α- 32 P]dCTP,the chitinase gene of PaorNPV was localized on the fragments of BamH I-D,EcoR I-A,Hind Ⅲ-A,Pst I-D and Xho I-G/H respectively.At the same time,the fragment of BamH I-D containing the chiA has been cloned.
文摘蜀柏毒蛾(Parocneria orienta)是近年来我省柏木林区发生和危害十分严重的食叶性害虫。为了探索一条柏木林区蜀柏毒蛾生物防治的有效途径,解决蜀柏毒蛾防治(主要是化学防治)与桑蚕业的冲突,1989年我们在金堂采集、分离到1株蜀柏毒蛾病毒新毒株,经中国科学院武汉病毒所鉴定为蜀柏毒蛾核型多角体病毒(Parocneria orienta Nunclear PolyedrosisVrius简称PoNPV)。为了研究该病毒的开发利用价值,1990年进行了PoNPV毒力的生物测定。现将研究结果报道如下。
