Bioassay of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab.The results showed that 1%VBL,12.5 μL·L-1 Chlorbenz...Bioassay of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab.The results showed that 1%VBL,12.5 μL·L-1 Chlorbenzuron,15 μL·L-1 Trichlorphon had better synergistic effect.Spraying of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon and PoNPV to control 2～3 instar larvae of second generation in field,the concentrations of PoNPV were 8×106 PIB·mL-1,4×106 PIB·mL-1,2×106 PIB·mL-1,1×106 PIB·mL-1,5×105 PIB·mL-1,there were 20 treatments.It was indicated that when the concentration was the same,the effect of PoNPV added synergists was better than that without synergists.展开更多
The gene library of Parocneria orienta nuclear polyhedrovirus(PaorNPV)was partly constructed by cloing some fragments of BamH I and EcoR I of PaorNPV genomic DNA into pGEM3Z(f-)as a vector.Using the chitinase gene of ...The gene library of Parocneria orienta nuclear polyhedrovirus(PaorNPV)was partly constructed by cloing some fragments of BamH I and EcoR I of PaorNPV genomic DNA into pGEM3Z(f-)as a vector.Using the chitinase gene of HearSNPV probed with[α- 32 P]dCTP,the chitinase gene of PaorNPV was localized on the fragments of BamH I-D,EcoR I-A,Hind Ⅲ-A,Pst I-D and Xho I-G/H respectively.At the same time,the fragment of BamH I-D containing the chiA has been cloned.展开更多
文摘Bioassay of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon was conducted on 2-instar larvae of Parocneria orientia in lab.The results showed that 1%VBL,12.5 μL·L-1 Chlorbenzuron,15 μL·L-1 Trichlorphon had better synergistic effect.Spraying of PoNPV+1%VBL,PoNPV+12.5 μL·L-1 Chlorbenzuron,PoNPV+15 μL·L-1 Trichlorphon and PoNPV to control 2～3 instar larvae of second generation in field,the concentrations of PoNPV were 8×106 PIB·mL-1,4×106 PIB·mL-1,2×106 PIB·mL-1,1×106 PIB·mL-1,5×105 PIB·mL-1,there were 20 treatments.It was indicated that when the concentration was the same,the effect of PoNPV added synergists was better than that without synergists.
文摘The gene library of Parocneria orienta nuclear polyhedrovirus(PaorNPV)was partly constructed by cloing some fragments of BamH I and EcoR I of PaorNPV genomic DNA into pGEM3Z(f-)as a vector.Using the chitinase gene of HearSNPV probed with[α- 32 P]dCTP,the chitinase gene of PaorNPV was localized on the fragments of BamH I-D,EcoR I-A,Hind Ⅲ-A,Pst I-D and Xho I-G/H respectively.At the same time,the fragment of BamH I-D containing the chiA has been cloned.
