目的探讨血必净联合血液透析治疗蜂蜇伤致急性肾损伤(AKI)的临床效果。方法选取2011年2月—2014年10月于攀枝花学院临床医学院、攀枝花中心医院及攀枝花攀钢集团总医院收治的蜂蜇伤致AKI患者252例为研究对象,根据治疗方法不同,将患者分...目的探讨血必净联合血液透析治疗蜂蜇伤致急性肾损伤(AKI)的临床效果。方法选取2011年2月—2014年10月于攀枝花学院临床医学院、攀枝花中心医院及攀枝花攀钢集团总医院收治的蜂蜇伤致AKI患者252例为研究对象,根据治疗方法不同,将患者分为观察组(132例)和对照组(120例)。两组入院后均给予支持对症治疗,观察组予血必净50 ml+5%葡萄糖溶液250 ml静脉滴注,2次/d,连续7 d。两组患者均采用血液透析治疗,根据病情变化确定透析次数。分别于治疗前及治疗后3、7 d晨起抽取静脉血,测定血尿素氮(BUN)、血肌酐(Scr)、血清胱抑素C(Cys-C)及C反应蛋白(CRP)水平。根据患者出院时Scr水平评价临床疗效,记录少尿期持续时间及住院时间。结果治疗前后与治疗方式在BUN、Scr、Cys-C、CRP水平上存在交互作用,治疗前后在BUN、Scr、Cys-C、CRP水平上主效应显著,治疗方式在BUN、Scr、Cys-C、CRP水平上主效应显著(P<0.05)。观察组治疗后3、7 d BUN、Scr、Cys-C、CRP水平均低于治疗前(P<0.05)。观察组治疗后3、7 d BUN、Scr、Cys-C、CRP水平均低于对照组(P<0.05)。观察组总有效率高于对照组,少尿期持续时间和住院时间较对照组缩短(P<0.05)。结论血必净联合血液透析治疗蜂蛰伤所致AKI患者,可明显降低体内炎症状态,改善患者肾功能,缩短住院时间。展开更多
Bee venom phospholipase A2(BvPLA2) is a lipolytic enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerophospholipids to liberate free fatty acids and lysophospholipids.In this work,a new BvPLA2(AccPLA2...Bee venom phospholipase A2(BvPLA2) is a lipolytic enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerophospholipids to liberate free fatty acids and lysophospholipids.In this work,a new BvPLA2(AccPLA2) gene from the Chinese honeybee(Apis cerana cerana) venom glands was inserted into bacmid to construct a recombinant transfer vector.Tn-5B-4(Tn) cells were transfected with the recombinant bacmid DNA for expression.Sodium dodecylsulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis revealed a double band with molecular weights of 16 and 18 kDa.Products of hexahistidine AccPLA2 fusion protein accumulated up to 5.32% of the total cellular proteins.The AccPLA2 fusion protein was cross reactive with the anti-AmPLA2(BvPLA2 of the European honeybee,Apis mellifera) polyclonal serum.The reaction resulted in a double glycosylation band,which agrees with the band generated by the native AmPLA2 in Western blot analysis.The PLA2 activity of the total extracted cellular protein in the hydrolyzing egg yolk is about 3.16 μmol/(min·mg).In summary,the recombinant AccPLA2 protein,a native BvPLA2-like structure with corresponding biological activities,can be glycosylated in Tn cells.These findings provided fundamental knowledge for potential genetic engineering to produce AccPLA2 in the pharmaceutical industry.展开更多
文摘目的探讨血必净联合血液透析治疗蜂蜇伤致急性肾损伤(AKI)的临床效果。方法选取2011年2月—2014年10月于攀枝花学院临床医学院、攀枝花中心医院及攀枝花攀钢集团总医院收治的蜂蜇伤致AKI患者252例为研究对象,根据治疗方法不同,将患者分为观察组(132例)和对照组(120例)。两组入院后均给予支持对症治疗,观察组予血必净50 ml+5%葡萄糖溶液250 ml静脉滴注,2次/d,连续7 d。两组患者均采用血液透析治疗,根据病情变化确定透析次数。分别于治疗前及治疗后3、7 d晨起抽取静脉血,测定血尿素氮(BUN)、血肌酐(Scr)、血清胱抑素C(Cys-C)及C反应蛋白(CRP)水平。根据患者出院时Scr水平评价临床疗效,记录少尿期持续时间及住院时间。结果治疗前后与治疗方式在BUN、Scr、Cys-C、CRP水平上存在交互作用,治疗前后在BUN、Scr、Cys-C、CRP水平上主效应显著,治疗方式在BUN、Scr、Cys-C、CRP水平上主效应显著(P<0.05)。观察组治疗后3、7 d BUN、Scr、Cys-C、CRP水平均低于治疗前(P<0.05)。观察组治疗后3、7 d BUN、Scr、Cys-C、CRP水平均低于对照组(P<0.05)。观察组总有效率高于对照组,少尿期持续时间和住院时间较对照组缩短(P<0.05)。结论血必净联合血液透析治疗蜂蛰伤所致AKI患者,可明显降低体内炎症状态,改善患者肾功能,缩短住院时间。
基金Project (No 2007AA10Z324) supported by the National High-Tech Research and Development Program (863) of China
文摘Bee venom phospholipase A2(BvPLA2) is a lipolytic enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerophospholipids to liberate free fatty acids and lysophospholipids.In this work,a new BvPLA2(AccPLA2) gene from the Chinese honeybee(Apis cerana cerana) venom glands was inserted into bacmid to construct a recombinant transfer vector.Tn-5B-4(Tn) cells were transfected with the recombinant bacmid DNA for expression.Sodium dodecylsulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis revealed a double band with molecular weights of 16 and 18 kDa.Products of hexahistidine AccPLA2 fusion protein accumulated up to 5.32% of the total cellular proteins.The AccPLA2 fusion protein was cross reactive with the anti-AmPLA2(BvPLA2 of the European honeybee,Apis mellifera) polyclonal serum.The reaction resulted in a double glycosylation band,which agrees with the band generated by the native AmPLA2 in Western blot analysis.The PLA2 activity of the total extracted cellular protein in the hydrolyzing egg yolk is about 3.16 μmol/(min·mg).In summary,the recombinant AccPLA2 protein,a native BvPLA2-like structure with corresponding biological activities,can be glycosylated in Tn cells.These findings provided fundamental knowledge for potential genetic engineering to produce AccPLA2 in the pharmaceutical industry.
