A hybrid peptide gene was designed and synthesized. Its encoding peptide is constructed from residues 3～14 of magainin and residues 1～13 of melittin. The MA E gene was cloned into plasmids pUC18 and pBV220. By DNA s...A hybrid peptide gene was designed and synthesized. Its encoding peptide is constructed from residues 3～14 of magainin and residues 1～13 of melittin. The MA E gene was cloned into plasmids pUC18 and pBV220. By DNA sequencing, the whole sequences of this gene is confirmed to be correct. The recombinant plasmid pBMA\|E was expressed in \%E.coli\% DH5α. A gene product band can be seen with Tricine\|SDS\|PAGE. The MA E hybrid peptide was purified by immobilized metal affinity chromatography. Bioactivity assay was carried out in liquid turbidity method. The bactericide value to \%E.coli\% K 12 D 31 is 0.182.展开更多
文摘A hybrid peptide gene was designed and synthesized. Its encoding peptide is constructed from residues 3～14 of magainin and residues 1～13 of melittin. The MA E gene was cloned into plasmids pUC18 and pBV220. By DNA sequencing, the whole sequences of this gene is confirmed to be correct. The recombinant plasmid pBMA\|E was expressed in \%E.coli\% DH5α. A gene product band can be seen with Tricine\|SDS\|PAGE. The MA E hybrid peptide was purified by immobilized metal affinity chromatography. Bioactivity assay was carried out in liquid turbidity method. The bactericide value to \%E.coli\% K 12 D 31 is 0.182.
