体外实验探讨蜜炙紫菀水煎剂对人乳腺癌MCF-7细胞的影响

目的探讨不同药物浓度的蜜炙紫菀水煎剂在体外实验中对乳腺癌MCF-7细胞的增殖、周期、迁移、凋亡的影响。方法通过MTT实验观察药物的处理对乳腺癌MCF-7细胞的生长与增殖情况的影响,采用流式细胞术研究蜜炙紫菀水煎剂对乳腺癌MCF-7细胞的增殖、周期和凋亡的影响,通过划痕实验观察蜜炙紫菀水煎剂对乳腺癌MCF-7细胞迁移的影响。结果 MTT实验结果发现,不同浓度的蜜炙紫菀水煎剂(25、30、35、40、80 mg/ml)对乳腺癌MCF-7细胞的增殖具有明显的抑制作用,抑制率分别为2.19%、12.58%、14.36%、15.96%、54.69%。流式细胞术的结果显示,与空白对照组相比较,蜜炙紫菀水煎剂可以使乳腺癌MCF-7细胞周期的各个时相(G1、S和G2期)的细胞所占百分数发生改变。浓度为25 mg/ml和35 mg/ml的蜜炙紫菀水煎剂能使G1期中细胞所占的百分数增加,且两个浓度的细胞凋亡率都大于空白对照组。划痕实验结果显示浓度为25 mg/ml和35 mg/ml蜜炙紫菀水煎剂组较空白对照组划痕愈合率偏低。结论不同浓度的蜜炙紫菀水煎剂在体外实验中对乳腺癌MCF-7细胞的增殖具有一定的抑制作用,同时能够将细胞的增殖周期阻滞在G1期,促进MCF-7细胞的凋亡。一定浓度的蜜炙紫菀水煎剂对MCF-7细胞的迁移具有抑制作用,为临床应用中药紫菀治疗乳腺癌疾病提供实验基础。

蜜炙紫菀水煎剂对大肠癌LOVO细胞凋亡及迁移的影响

目的：探讨蜜炙紫菀水煎剂对大肠癌LOVO细胞凋亡及迁移的影响方法：应用荧光显微镜观察DAPI染色后蜜炙紫菀水煎剂作用前后对LOVO细胞凋亡情况的影响;流式细胞仪检测蜜炙紫菀水煎剂对LOVO细胞凋亡率的影响;划痕实验和Transwell小室法检测不同质量浓度的蜜炙紫菀水煎剂处理LOVO细胞后对大肠癌LOVO细胞的迁移情况的影响。结果：荧光显微镜下观察发现蜜炙紫菀水煎剂实验组和空白对照组均未见细胞核凝集、染色体碎裂等细胞凋亡改变;流式细胞术结果也发现,不同浓度蜜炙紫菀水煎剂处理LOVO细胞48 h后,在细胞周期图G0期均未出现二倍峰,且凋亡率未见明显改变;浓度为5～30 mg·m L-1的蜜炙紫菀水煎剂处理LOVO细胞24、48 h后,与对照组相比,划痕实验结果显示大肠癌LOVO细胞的划痕距离较大,划痕未见明显愈合,均可以抑制大肠癌LOVO细胞的迁移,在蜜炙紫菀水煎剂浓度为40 mg·m L-1和80 mg·m L-1时,会导致大肠癌LOVO细胞非凋亡性死亡,Transwell小室结果显示,药物实验组穿透Transwell小室上层膜到达下室的LOVO细胞数明显少于空白对照组,蜜炙紫菀水煎剂浓度为30 mg·m L-1的实验组迁移率最低,为50.7%。结论：不同浓度的蜜炙紫菀水煎剂对大肠癌LOVO细胞的凋亡没有影响,但在高浓度时可以导致非凋亡性细胞死亡。同时,确定一定浓度的蜜炙紫菀水煎剂可以有效抑制大肠癌LOVO细胞的迁移,为有效预防大肠癌复发提供实验基础。

不同浓度蜜炙紫菀水煎剂对大肠癌LOVO细胞增殖和周期的影响

目的:探讨不同浓度蜜炙紫菀水煎剂对大肠癌LOVO细胞增殖、周期的影响。方法:光学显微镜下观察蜜炙紫菀水煎剂对LOVO细胞形态的影响;利用MTT观察药物处理后对LOVO细胞的生长增殖情况的影响;采用流式细胞术检测蜜炙紫菀水煎剂对大肠癌LOVO细胞增殖周期的影响。结果:光学显微镜和MTT结果发现,低浓度的蜜炙紫菀水煎剂(≤20 mg·mL")对大肠癌LOVO细胞的增殖具有明显的促进作用,增殖率可达到99.7%;光学显微镜下可见细胞生长状态良好,细胞死亡少。高浓度的蜜炙紫菀水煎剂(>20 mg·mL^(-1))对大肠癌LOVO细胞的增殖具有明显的抑制作用,抑制率达到80.34%以上;光学显微镜下可见细胞的形态改变,细胞大量死亡。流式细胞术结果显示,蜜炙紫菀水煎剂可以使大肠癌LOVO细胞周期各个时相(G1期、S期和G2期)的细胞所占百分数发生改变。10 mg·mL^(-1)和20 mg·mL^(-1)的蜜炙紫菀水煎荆使S期中细胞所占的百分数增加;30 mg·mL^(-1)蜜炙紫菀水煎剂使S期细胞所占的百分数明显减少,G2期细胞百分数明显增加。结论:蜜炙紫菀水煎荆对大肠癌LOVO细胞增殖的作用是双向的,低浓度(≤20 mg·mL^(-1))的蜜炙紫菀水煎剂具有明显的促进LOVO细胞增殖作用;高浓度(>20 mg·mL^(-1))的蜜炙紫菀水煎剂对大肠癌LOVO细胞的增殖具有明显的抑制作用,且可能是通过G2期阻滞来抑制大肠癌LOVO细胞增殖的,这为大肠癌的临床治疗提供了新的思路。

双柏水蜜膏对粘连性肠梗阻患者炎性因子TNF-a、IL-6的影响

目的观察中药外敷配合常规治疗方法对粘连性肠梗阻患者炎性因子的影响。方法将60例患者随机分为2组。A组(对照组)28例,采用胃肠减压、抗感染及支持疗法的西医保守治疗方法 ;B组(治疗组)32例,在A组治疗基础上结合双柏散(药物组成:侧柏叶、大黄、黄柏、泽兰、薄荷)外敷。观察2组临床疗效和治疗前后TNF-a(肿瘤坏死因子)、IL-6(白细胞介素-6)前后水平变化。结果 2组治疗后TNF-a、IL-6水平均降低,其中治疗组降低更显著(与对照组比较,P<0.05);治疗组患者腹痛消失时间、肛门排气、排便时间、X线气液平面消失时间与对照组比较均缩短,差异均有非常显著性意义(P<0.05)。结论中西医结合治疗粘连性肠梗阻疗效优于单纯西医常规治疗,运用外治法能降低粘连性肠梗阻患者血中TNF-a、IL-6水平,具有免疫调节、减轻炎症反应从而提高临床疗效。

桃品种颐红水蜜在贵州石阡栽培技术

经过近8年的引种试栽观察,桃品种颐红水蜜在贵州省石阡县生长结果良好,表现为中晚熟,结果早、丰产,果个大、整齐,果面浓红、光亮美观,味美、香脆爽口,含糖量高,耐贮运,经济效益显著,同时总结出颐红水蜜在当地的优质丰产栽培技术,可在适宜栽培水蜜桃的区域推广。

双柏水蜜散配合暖身贴外敷治疗慢性盆腔炎临床护理观察

目的:探讨中药双柏散外敷配合暖身贴治疗女性慢性盆腔炎的护理效果。方法:将30例慢性盆腔炎患者随机分为观察组和对照组,每组各15例。观察组采用口服中药汤剂的同时,给予静滴复方丹参注射液加双柏散外敷治疗。对照组采用中药汤剂口服的同时,给予复方丹参注射液静滴加暖身贴外敷治疗。结果:观察组和对照组的有效率分别为90%和75%,观察组优于对照组。结论:双柏水蜜散配合暖身贴外敷辅助治疗女性慢性盆腔炎行之有效。

水蜜桃新秀颐红水蜜

本县引进颐红水蜜桃试种成功。该品种早果、丰产、果大整齐,果面浓红,光亮美观,肉质脆嫩,含糖量高,耐贮耐运。 颐红水蜜桃第一年栽植,第二年挂果,第三年丰产。平均单果重200克左右。

双柏水蜜膏治疗急性闭合性肾损伤的临床观察

目的观察双柏水蜜膏配合西医常规治疗急性闭合性肾损伤的临床疗效。方法将58例急性闭合性肾损伤患者随机分为2组,对照组28例予以常规西医治疗;治疗组30例在对照组治疗的基础上,加用双柏水蜜膏局部外敷。观察2组患者住院时间、肾被膜及肾周血肿吸收时间、尿液红细胞消失时间及外周血白细胞变化情况。结果治疗组住院时间、肾被膜及肾周血肿吸收时间、尿液红细胞消失时间均较对照组明显缩短(P<0.05),治疗后外周血白细胞数量下降明显优于对照组(P<0.01)。结论双柏水蜜膏辅助治疗急性闭合性肾损伤疗效优于单纯西医常规治疗。

柠檬制品Vc及挥发性成分测定

为了比较和分析柠檬制品柠檬蜜和鲜柠檬水中的Vc和挥发性成分的变化,分别采用2,4-二硝基苯肼法和固相微萃取结合气相色谱-质谱(SPME-GC-MS)分析技术,对柠檬蜜和新鲜柠檬水中的Vc含量和挥发性成分进行分析测定.结果表明:柠檬蜜水和新鲜柠檬水中Vc含量分别为11.7 mg/kg、17.6 mg/kg;其挥发性成分种类和相对含量存在着一定的差别,两种样品共鉴定出31种化合物,柠檬蜜水和鲜柠檬水挥发性成分种类分别为25种和20种,主要包括烯烃、醇类、醛类、酯类和酮类等物质.结合感官综合比较,柠檬蜜较鲜柠檬水风味浓郁,食用更为简单,但其Vc含量较少.

中医综合治疗子宫内膜异位症合并不孕25例

目的:观察中医综合治疗子宫内膜异位症合并不孕症的临床疗效。方法:将50例患者随机分为两组,治疗组给予中医综合治疗,对照组给予内美通口服治疗,均治疗3个月,随访1年,观察两组临床症状、体征疗效及妊娠情况,并检测血液流变学水平。结果:两组总有效率分别为72%和68%,差异无统计学意义(P>0.05);随访1年后,治疗组妊娠率为64%,对照组妊娠率为36%,差异有显著性(P<0.05);治疗组血液粘度改善情况优于对照组;治疗组方法安全,无明显毒性和不良反应。结论:中医综合疗法治疗子宫内膜异位症合并不孕症患者,能够减轻和改善症状,促进受孕,且副作用小,无明显不良反应。

Synergistic Effects of Nitric Oxide and Harvest Maturity on Quality of Honey Peach during Cold Storage

In this study, peaches of cv. ＇Sui Yuan＇ with different maturities were treated with 0.05 mM sodium nitroprusside （SNP）, a nitric oxide donor, for 5 min, at 28℃, and then stored at 0-1.5℃ for 30 days. Untreated fruits stored under the same conditions were used as control fruits in the experiment. The browning level, activities of polyphenol oxidase （PPO）, peroxydase （POD） and superoxide dismutase （SOD）, and quality parameters （firmness, titratable acid, total soluble solids and total sugar） were analyzed. The results showed that the firmness of SNP-treated fruit at different stage of maturity were higher than that of the control fruit after storage for 30 days. However, no significant effect was found on total soluble solid content. The titratable acid content of low maturity fruit was higher in SNP-treated one than that of the control, but the titratable acid content of high maturity fruit treated by SNP was lower than that of the control on 10th and 30th day. In addition, the combination of SNP treatment and high maturity resulted in higher activities of SOD, lower activity of PPO and POD, lower browning level. Therefore, the combination of high maturity and SNP treatment might be a useful method to well maintain honey peach fruit quality during cold storage.

中药灌肠、外敷、针刺对粘连性肠梗阻患者炎性因子TNF-α、IL-6的影响

目的:观察中药灌肠、外敷、针刺配合常规治疗方法对粘连性肠梗阻患者炎性因子的影响。方法:将60例患者随机分为两组。A组(对照组)28例,采用胃肠减压、抗感染及支持疗法的西医保守治疗方法;B组(治疗组)32例,在A组治疗基础上结合通腑泻热合剂保留灌肠同时用双柏散外敷腹部疼痛部位及针刺足三里。观察两组临床疗效和治疗前后TNF-α(肿瘤坏死因子)、IL-6(白细胞介素-6)前后水平变化。结果:两组治疗后TNF-α、IL-6水平均降低,其中治疗组降低更显著(与对照组比较,P<0.05);治疗组患者腹痛消失时间、肛门排气、排便时间、X线气液平面消失时间与对照组比较均缩短,差异均有非常显著性意义(P<0.05)。结论:中西医结合治疗粘连性肠梗阻疗效优于单纯西医常规治疗,运用外治法能降低粘连性肠梗阻患者血中TNF-α、IL-6水平,具有免疫调节、减轻炎症反应从而提高临床疗效的作用。

外敷加味双柏水蜜治疗急性闭合性软组织损伤60例

我们于2009年8月至2010年10月采用外敷加味双柏水蜜治疗急性闭合性软组织损伤60例,取得了较满意的临床疗效,现报告如下。

Effects of Different Storage Methods on the Fruit Quality and Physiological Changes in Yihong Honey Peach

Taking Yihong,the main cultivar of mid-ripening honey peaches(Prunus persica)in Hunan,as material,this study explored the effects of different storage methods on the fruit quality and physiological changes in Yihong honey peach by the combination of polyethylene film packaging with low-temperature storage.The results showed that the weight loss rate of packaged storage at normal temperature(T1)was significantly lower than that of unpackaged storage at normal temperature(CK),but there was no significant difference in other indicators.In the middle of storage,the respiration rate and ethylene release of packaged storage at 0℃(T3)were extremely significantly lower or significantly lower than those of unpackaged storage at 0℃(T2),packaged storage at 0℃after cold adapting at 8℃for 5 d(T4),and packaged storage in a fluctuating temperature range of 0~3.5℃(T5).After storage,the structure of the middle lamella in the cell wall of T3 was compact and uniform,and the mitochondrial structure was complete,while the cell walls and mitochondria of T4 and T5 were severely deformed and disintegrated.The activities of antioxidant enzymes peroxidase(POD)and superoxide dismutase(SOD)of T3 was significantly higher than those of T4 and T5,and the content of malondialdehyde(MDA)and polyphenol oxidase(PPO)of T3 was also the lowest.Therefore,0°C packaged storage can effectively delay the senescence of Yihong honey peach and prolong its freshness period.

Kinetics of Reductive Leaching of Low-grade Pyrolusite with Molasses Alcohol Wastewater in H_2SO_4

The kinetics of reductive leaching of manganese from low grade pyrolusite in dilute sulfuric acid in the presence of molasses alcohol wastewater was investigated. The shrinking core model was applied to quantify the effects of reaction parameters on leaching rate. The leaching rate increases with reaction temperature, concentrations of H 2 SO 4 and organic matter in molasses alcohol wastewater increase and ore particle size decreases. The leaching process follows the kinetics of a shrinking core model and the apparent activation energy is 57.5 kJ·mol –1 . The experimental results indicate a reaction order of 0.52 for H2SO4 concentration and 0.90 for chemical oxygen demand (COD) of molasses alcohol wastewater. It is concluded that the reductive leaching of pyrolusite with molasses alcohol wastewater is controlled by the diffusion through the ash/inert layer composed of the associated minerals.

Sweet false stalk crusher

After finishing picking the banana, how to tackle banana pesudostem has long been a thorny problem. According to banana pesudostem＇s characteristics of more crassitude stem, high moisture content and heavy fiber density, this paper proposes a efficient, energy-saving and low-loss device for crushing banana pesudostems and returning field.

Water Soluble Propolis and Royal Jelly Enhance the Antimicrobial Activity of Honeys and Promote the Growth of Human Macrophage Cell Line

Due to the overuse and misuse of antibiotic, an increase in antibiotic resistance of pathogenic bacteria is evolving. Attention should be focused on natural alternatives to antibiotics, like propolis, royal jelly （R J） and honeys. They all have strong antibacterial properties due to the active substances they contain. This study investigated the effect of combination of water soluble propolis （WSP） Greitl20 or fresh royal jelly （F-RJ） （MiZigoj） and Forest honeys as antibacterial against Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Acinetobacter baumanii, Staphylococcus aureus, methicillin resistant Staphylococcus aureus （MRSA）, Streptococcus pyogenes, Streptococcus agalactiae and Candida albicans. These substances are also cell growth promoters for human macrophage （TLT） cell line. WSP Greitl20, F-RJ （M） and different Forest honeys were prepared in saline as 10% solutions. The antimicrobial activity was expressed as the minimal inhibitory concentration （MIC） in mg/mL. The growth promotion activity was measured at optical density （OD） 595 nm. The combination ofWSP Greitl20 with different Forest honeys is better than F-RJ （M） in same combination with different Forest honeys. The best antibacterial/antifungal activity was found with the combination of 10% WSP Greit 120 in the Forest honey （1：10） from Italy or Spain. When measuring the growth promoting activity of TLT cell line, the best activity was detected at the combination of 10% WSP Greitl20 in the Forest honey from Italy （GI3 = 0.796 ± 0.014 and GI5 = 1.133± 0.022）. Antimicrobial and growth promoting activities are correlated and WSP-dependent.

The recent disciplinal progresses of agricultural entomology in China

In this paper, four recent advances and achievements of China in agricultural insect research, namely, on the genome of silkworm (Bombyx mori Linnaeus), on the geographical differentiation and regional migration of cotton bollworm (Helicoverpa armigera (Hübner)), on the standardized monitoring techniques for safety of honey bee (Apis mellifera Linnaeus) products, and on the virus transmission property of small brown planthopper (Laodelphax striatellus (Fallén)) as well as the interactions between vector and rice stripe virus (RSV), were reported. All of these researches are very important for controlling agricultural insect pests and the diseases they transmit, accelerating the molecular biological research of silkworm, and promoting the international trade of honey bee products. Most of these achievements mentioned above have got the national, provincial, ministerial or municipal awards on science and technology.