3D modeling of geological bodies based on 3D seismic data is used to define the shape and volume of the bodies, which then can be directly applied to reservoir prediction, reserve estimation, and exploration. However,...3D modeling of geological bodies based on 3D seismic data is used to define the shape and volume of the bodies, which then can be directly applied to reservoir prediction, reserve estimation, and exploration. However, multiattributes are not effectively used in 3D modeling. To solve this problem, we propose a novel method for building of 3D model of geological anomalies based on the segmentation of multiattribute fusion. First, we divide the seismic attributes into edge- and region-based seismic attributes. Then, the segmentation model incorporating the edge- and region-based models is constructed within the levelset- based framework. Finally, the marching cubes algorithm is adopted to extract the zero level set based on the segmentation results and build the 3D model of the geological anomaly. Combining the edge-and region-based attributes to build the segmentation model, we satisfy the independence requirement and avoid the problem of insufficient data of single seismic attribute in capturing the boundaries of geological anomalies. We apply the proposed method to seismic data from the Sichuan Basin in southwestern China and obtain 3D models of caves and channels. Compared with 3D models obtained based on single seismic attributes, the results are better agreement with reality.展开更多
A recombinant plasmid (pcMTsGH) containing the common carp methlothionein promoter (cMT) and salmon growth hormone gene (sGH) was microinjected into common carp fertilized eggs and embryos to optimize the conditions f...A recombinant plasmid (pcMTsGH) containing the common carp methlothionein promoter (cMT) and salmon growth hormone gene (sGH) was microinjected into common carp fertilized eggs and embryos to optimize the conditions for producing transgenic fish. Several key elements were found to have significant effects on the integration of exogenous gene. These elements include forms and doses of pcMTsGH gene microinjected, stages of embryo at which microinjection conducted and pretreatment of fertilized eggs for microinjection. The results show that, in producing transgenic fish, (1) the best time for microinjection is late onecell stage, (2) the most appropriate dose of pcMTsGH gene for microinjection is around 106~107 copies; (3) the hatching rate rises up when the gene is microinjected into the eggs whose chorions are removed, but the integration rate has no significant changes before and after the removal of egg chorions.展开更多
基金supported by the National Natural Science Foundation of China(No.41604107)the Scientific Research Staring Foundation of University of Electronic Science and Technology of China(No.ZYGX2015KYQD049)
文摘3D modeling of geological bodies based on 3D seismic data is used to define the shape and volume of the bodies, which then can be directly applied to reservoir prediction, reserve estimation, and exploration. However, multiattributes are not effectively used in 3D modeling. To solve this problem, we propose a novel method for building of 3D model of geological anomalies based on the segmentation of multiattribute fusion. First, we divide the seismic attributes into edge- and region-based seismic attributes. Then, the segmentation model incorporating the edge- and region-based models is constructed within the levelset- based framework. Finally, the marching cubes algorithm is adopted to extract the zero level set based on the segmentation results and build the 3D model of the geological anomaly. Combining the edge-and region-based attributes to build the segmentation model, we satisfy the independence requirement and avoid the problem of insufficient data of single seismic attribute in capturing the boundaries of geological anomalies. We apply the proposed method to seismic data from the Sichuan Basin in southwestern China and obtain 3D models of caves and channels. Compared with 3D models obtained based on single seismic attributes, the results are better agreement with reality.
文摘A recombinant plasmid (pcMTsGH) containing the common carp methlothionein promoter (cMT) and salmon growth hormone gene (sGH) was microinjected into common carp fertilized eggs and embryos to optimize the conditions for producing transgenic fish. Several key elements were found to have significant effects on the integration of exogenous gene. These elements include forms and doses of pcMTsGH gene microinjected, stages of embryo at which microinjection conducted and pretreatment of fertilized eggs for microinjection. The results show that, in producing transgenic fish, (1) the best time for microinjection is late onecell stage, (2) the most appropriate dose of pcMTsGH gene for microinjection is around 106~107 copies; (3) the hatching rate rises up when the gene is microinjected into the eggs whose chorions are removed, but the integration rate has no significant changes before and after the removal of egg chorions.