背景:后外侧融合与环周融合作为修复腰椎滑脱症主要方式,各具优势。目的:系统评价后外侧植骨融合与360°环周融合修复腰椎滑脱症的安全性和有效性。方法:计算机检索Cochrane图书馆、Pub Med数据库、Web of Knowledge、中国科学引文...背景:后外侧融合与环周融合作为修复腰椎滑脱症主要方式,各具优势。目的:系统评价后外侧植骨融合与360°环周融合修复腰椎滑脱症的安全性和有效性。方法:计算机检索Cochrane图书馆、Pub Med数据库、Web of Knowledge、中国科学引文数据库、中文科技期刊数据库、中国学术期刊网络出版总库数据库、万方数据库、中国生物医学文献数据库,所有数据库均检索至2014年7月。查找后外侧植骨融合与360°环周融合修复腰椎滑脱症的随机对照试验,使用Rev Man 5.2软件进行Meta分析。结果与结论:最终纳入3个随机对照试验,共336例患者,后外侧植骨融合组175例,360°环周融合组161例。Meta分析结果显示,随访6个月(MD=8.08,95%CI:1.23至14.94,P=0.02)、12个月(MD=6.72,95%CI:6.02至7.42,P<0.000 01)、24个月(MD=4.94,95%CI:4.30至5.57,P<0.000 01)患者Oswestry功能障碍指数比较,360°环周融合组优于后外侧植骨融合组;手术时间(MD=-91.15,95%CI:-133.17至-49.14,P<0.000 1)、住院时间(MD=-0.98,95%CI:-1.85至-0.11,P=0.03)方面,后外侧植骨融合组明显优于360°环周融合组;躯体功能评分及躯体疼痛评分,随访6,12,24个月360°环周融合组均明显优于后外侧植骨融合组(P<0.05)。在术中出血量(MD=-165.51,95%CI:-375.89至44.86,P=0.12)、并发症发生率(OR=1.16,95%CI:0.51至2.62,P=0.72)及目测类比评分(MD=0.20,95%CI:-0.36至0.76,P=0.48)方面两组差异无显著性意义。提示360°环周融合可显著降低患者Oswestry功能障碍指数、躯体功能评分、躯体疼痛指数,但手术时间及住院时间较长,临床实践应根据腰椎滑脱患者的实际情况选择最优修复方案。展开更多
[Objective] This study aimed to obtain recombinant alpha-bungarotoxin （a-BG-0 gene fusion protein with biological activity and investiagte its fusion expression. [Method] The plasmid pGEX-a-BGT was transformed into E...[Objective] This study aimed to obtain recombinant alpha-bungarotoxin （a-BG-0 gene fusion protein with biological activity and investiagte its fusion expression. [Method] The plasmid pGEX-a-BGT was transformed into E coil BL21 （DE3） and BL21 （DE3） plysS host bacteria to identify the optimal engineering strain. Fusion expression of the optimal engineering strain was induced, in order to optimize the induced expression conditions of the soluble fusion protein. [Result] JP-a-BGT was identified as the optimal engineering strain, which could express fusion protein after induced by IPTG. The optimal induced expression conditions of the soluble fusion protein were investigatect JP-a-BGT was incubated at 37 ℃ for 2.5 h and induced with 0.50 mmol4. IPTG for 4 h at 22 ℃, and the expression level of the soluble fusion protein reached 18.42%. [Conclusion] This study laid a solid foundation for the subsequent purification of fusion proteins and the separation and purification of a-BGT.展开更多
A method for the multi target locating and tracking with the multi sensor in a field artillery system is studied. A general modeling structure of the system is established. Based on concepts of cluster and closed ba...A method for the multi target locating and tracking with the multi sensor in a field artillery system is studied. A general modeling structure of the system is established. Based on concepts of cluster and closed ball, an algorithm is put forward for multi sensor multi target data fusion and an optimal solution for state estimation is presented. The simulation results prove the algorithm works well for the multi stationary target locating and the multi moving target tracking under the condition of the sparse target environment. Therefore, this method can be directly applied to the field artillery C 3I system.展开更多
As a frequently-used roadbed filler,soil-rock mixture is often in the environment of freeze-thaw cycles and different confining pressures.In order to study the freeze-thaw damage mechanism of elastic modulus of soil-r...As a frequently-used roadbed filler,soil-rock mixture is often in the environment of freeze-thaw cycles and different confining pressures.In order to study the freeze-thaw damage mechanism of elastic modulus of soil-rock mixtures at different confining pressures,the concept of meso-interfacial freeze-thaw damage coefficient is put forward and the meso-interfacial damage phenomenon of soil-rock mixtures caused by the freeze-thaw cycle environment is concerned;a double-inclusion embedded model for elastic modulus of soil-rock mixtures in freezing-thawing cycle is proposed.A large triaxial test was performed and the influences of confining pressure and experimental factors on elastic modulus of soil-rock mixtures were obtained,and then the accuracy of the double-inclusion embedded model to predict the elastic modulus of soil-rock mixtures in freezing-thawing cycle is verified.Experiment results showed that as to soil-rock mixtures,with the increase of confining pressure,the elastic modulus increases approximately linearly.The most crucial factors to affect the elastic modulus are rock content and compaction degree at the same confining pressure;the elastic modulus increases with the increase of rock content and compactness;as the number of freeze-thaw cycles increases,the freeze-thaw damage coefficient of meso-structural interface and the elastic modulus decrease.展开更多
As a widely distributed geological and engineering material,the soil-rock mixture always undergoes frequentative and short-term freeze-thaw cycles in some regions.Its internal structure is destroyed seriously,but the ...As a widely distributed geological and engineering material,the soil-rock mixture always undergoes frequentative and short-term freeze-thaw cycles in some regions.Its internal structure is destroyed seriously,but the damage mechanism is not clear.Based on the damage factor,the damage research of properties of soil-rock mixture after different times of freeze-thaw cycles is investigated.Firstly,the size-distributed subgrade gravelly soil samples are prepared and undergo different times of freeze-thaw cycles periodically(0,3,6,10),and indoor large-scale triaxial tests are completed.Secondly,the degradation degree of elastic modulus is considered as a damage factor,and applied to macro damage analysis of soil-rock mixture.Finally,the mesoscopic simulation of the experiments is achieved by PFC3D,and the influence on strength between soil-rock particles caused by freeze-thaw cycles is analyzed.The results show that freeze-thaw cycles cause internal damage of samples by weakening the strength between mesoscopic soil-rock particles,and ultimately affect the macro properties.After freeze-thaw cycles,on the macro-scale,elastic modulus and shear strength of soil-rock mixture both decrease,and the decreasing degree is related to the times of cycles with the mathmatical quadratic form;on the meso-scale,freeze-thaw cycles mainly cause the degradation of the strength between soil-rock particles whose properties are different significantly.展开更多
Objective To present the operative results of 17patients with atlantoaxial diseases using transoral approach.Methods17patients received operation of atlantoaxial fusion using transoral approach.The average age is 27ye...Objective To present the operative results of 17patients with atlantoaxial diseases using transoral approach.Methods17patients received operation of atlantoaxial fusion using transoral approach.The average age is 27years old(12～47years).Results All patients had achieved primary healing without any complications.The fusion rate was100%.The average recovery rate(JOAscore system)of myelopathy was48%.Conclusions Transoral approach was an ideal approach for upper cervical diseases.The infection rate could be lowered dow n to the minimum if the patients were properly managed perioperatively.Atlantoaxial fu sion could be achieved through this a pproach without instrumentation.展开更多
computer music is a new type of multi-disciplinary integration of computer technology with traditional music, to meet the development needs of music education refom.Music education in the integration of computer music...computer music is a new type of multi-disciplinary integration of computer technology with traditional music, to meet the development needs of music education refom.Music education in the integration of computer music technology is imminent. The articles make the analysis on teaching mode of computer music courses and teaching environment constructed, and the paper proposed some viewpoint on the necessity and feasibility of computer music technology teaching,positive and negative impacts and future trends.The application of computer technology to music teaching in schools, not only solve the traditional teaching of the long-troubled, and can receive better teaching effect, which has a very important show significance. I hope to play a guidance and reference promotion and teaching of computer music technology in-depth.展开更多
文摘背景:后外侧融合与环周融合作为修复腰椎滑脱症主要方式,各具优势。目的:系统评价后外侧植骨融合与360°环周融合修复腰椎滑脱症的安全性和有效性。方法:计算机检索Cochrane图书馆、Pub Med数据库、Web of Knowledge、中国科学引文数据库、中文科技期刊数据库、中国学术期刊网络出版总库数据库、万方数据库、中国生物医学文献数据库,所有数据库均检索至2014年7月。查找后外侧植骨融合与360°环周融合修复腰椎滑脱症的随机对照试验,使用Rev Man 5.2软件进行Meta分析。结果与结论:最终纳入3个随机对照试验,共336例患者,后外侧植骨融合组175例,360°环周融合组161例。Meta分析结果显示,随访6个月(MD=8.08,95%CI:1.23至14.94,P=0.02)、12个月(MD=6.72,95%CI:6.02至7.42,P<0.000 01)、24个月(MD=4.94,95%CI:4.30至5.57,P<0.000 01)患者Oswestry功能障碍指数比较,360°环周融合组优于后外侧植骨融合组;手术时间(MD=-91.15,95%CI:-133.17至-49.14,P<0.000 1)、住院时间(MD=-0.98,95%CI:-1.85至-0.11,P=0.03)方面,后外侧植骨融合组明显优于360°环周融合组;躯体功能评分及躯体疼痛评分,随访6,12,24个月360°环周融合组均明显优于后外侧植骨融合组(P<0.05)。在术中出血量(MD=-165.51,95%CI:-375.89至44.86,P=0.12)、并发症发生率(OR=1.16,95%CI:0.51至2.62,P=0.72)及目测类比评分(MD=0.20,95%CI:-0.36至0.76,P=0.48)方面两组差异无显著性意义。提示360°环周融合可显著降低患者Oswestry功能障碍指数、躯体功能评分、躯体疼痛指数,但手术时间及住院时间较长,临床实践应根据腰椎滑脱患者的实际情况选择最优修复方案。
文摘[Objective] This study aimed to obtain recombinant alpha-bungarotoxin （a-BG-0 gene fusion protein with biological activity and investiagte its fusion expression. [Method] The plasmid pGEX-a-BGT was transformed into E coil BL21 （DE3） and BL21 （DE3） plysS host bacteria to identify the optimal engineering strain. Fusion expression of the optimal engineering strain was induced, in order to optimize the induced expression conditions of the soluble fusion protein. [Result] JP-a-BGT was identified as the optimal engineering strain, which could express fusion protein after induced by IPTG. The optimal induced expression conditions of the soluble fusion protein were investigatect JP-a-BGT was incubated at 37 ℃ for 2.5 h and induced with 0.50 mmol4. IPTG for 4 h at 22 ℃, and the expression level of the soluble fusion protein reached 18.42%. [Conclusion] This study laid a solid foundation for the subsequent purification of fusion proteins and the separation and purification of a-BGT.
文摘A method for the multi target locating and tracking with the multi sensor in a field artillery system is studied. A general modeling structure of the system is established. Based on concepts of cluster and closed ball, an algorithm is put forward for multi sensor multi target data fusion and an optimal solution for state estimation is presented. The simulation results prove the algorithm works well for the multi stationary target locating and the multi moving target tracking under the condition of the sparse target environment. Therefore, this method can be directly applied to the field artillery C 3I system.
基金Project(50908234)supported by the National Natural Science Foundation of China
文摘As a frequently-used roadbed filler,soil-rock mixture is often in the environment of freeze-thaw cycles and different confining pressures.In order to study the freeze-thaw damage mechanism of elastic modulus of soil-rock mixtures at different confining pressures,the concept of meso-interfacial freeze-thaw damage coefficient is put forward and the meso-interfacial damage phenomenon of soil-rock mixtures caused by the freeze-thaw cycle environment is concerned;a double-inclusion embedded model for elastic modulus of soil-rock mixtures in freezing-thawing cycle is proposed.A large triaxial test was performed and the influences of confining pressure and experimental factors on elastic modulus of soil-rock mixtures were obtained,and then the accuracy of the double-inclusion embedded model to predict the elastic modulus of soil-rock mixtures in freezing-thawing cycle is verified.Experiment results showed that as to soil-rock mixtures,with the increase of confining pressure,the elastic modulus increases approximately linearly.The most crucial factors to affect the elastic modulus are rock content and compaction degree at the same confining pressure;the elastic modulus increases with the increase of rock content and compactness;as the number of freeze-thaw cycles increases,the freeze-thaw damage coefficient of meso-structural interface and the elastic modulus decrease.
基金Project(50908234)supported by the National Natural Science Foundation of ChinaProject(2017G002-K)supported by the Key Subject of Science and Technology Research and Development Plan of China Railway General Corporation
文摘As a widely distributed geological and engineering material,the soil-rock mixture always undergoes frequentative and short-term freeze-thaw cycles in some regions.Its internal structure is destroyed seriously,but the damage mechanism is not clear.Based on the damage factor,the damage research of properties of soil-rock mixture after different times of freeze-thaw cycles is investigated.Firstly,the size-distributed subgrade gravelly soil samples are prepared and undergo different times of freeze-thaw cycles periodically(0,3,6,10),and indoor large-scale triaxial tests are completed.Secondly,the degradation degree of elastic modulus is considered as a damage factor,and applied to macro damage analysis of soil-rock mixture.Finally,the mesoscopic simulation of the experiments is achieved by PFC3D,and the influence on strength between soil-rock particles caused by freeze-thaw cycles is analyzed.The results show that freeze-thaw cycles cause internal damage of samples by weakening the strength between mesoscopic soil-rock particles,and ultimately affect the macro properties.After freeze-thaw cycles,on the macro-scale,elastic modulus and shear strength of soil-rock mixture both decrease,and the decreasing degree is related to the times of cycles with the mathmatical quadratic form;on the meso-scale,freeze-thaw cycles mainly cause the degradation of the strength between soil-rock particles whose properties are different significantly.
文摘Objective To present the operative results of 17patients with atlantoaxial diseases using transoral approach.Methods17patients received operation of atlantoaxial fusion using transoral approach.The average age is 27years old(12～47years).Results All patients had achieved primary healing without any complications.The fusion rate was100%.The average recovery rate(JOAscore system)of myelopathy was48%.Conclusions Transoral approach was an ideal approach for upper cervical diseases.The infection rate could be lowered dow n to the minimum if the patients were properly managed perioperatively.Atlantoaxial fu sion could be achieved through this a pproach without instrumentation.
文摘computer music is a new type of multi-disciplinary integration of computer technology with traditional music, to meet the development needs of music education refom.Music education in the integration of computer music technology is imminent. The articles make the analysis on teaching mode of computer music courses and teaching environment constructed, and the paper proposed some viewpoint on the necessity and feasibility of computer music technology teaching,positive and negative impacts and future trends.The application of computer technology to music teaching in schools, not only solve the traditional teaching of the long-troubled, and can receive better teaching effect, which has a very important show significance. I hope to play a guidance and reference promotion and teaching of computer music technology in-depth.
