以钩端螺旋体基因组DNA为模板,通过酶联聚合反应(PCR)得到钩端螺旋体中prmA的同源基因lip-rmA的全基因编码序列,并克隆到原核表达载体pET22b中。通过优化大肠杆菌培养和诱导条件,含目的蛋白的融合蛋白可溶表达量达到40 mg/L,约占菌体总...以钩端螺旋体基因组DNA为模板,通过酶联聚合反应(PCR)得到钩端螺旋体中prmA的同源基因lip-rmA的全基因编码序列,并克隆到原核表达载体pET22b中。通过优化大肠杆菌培养和诱导条件,含目的蛋白的融合蛋白可溶表达量达到40 mg/L,约占菌体总蛋白的40%。经Ni-NTA His Bind亲和柱纯化,得到纯度大于95%的目的蛋白。氨基酸序列同源性分析显示liPrmA与原核生物和真核生物的核糖体蛋白L11甲基化转移酶的功能域一级结构高度一致;活性分析显示,纯化的liPrmA有钩端螺旋体核糖体蛋白L11甲基化转移酶的活性。展开更多
Regarding the scale effects on propeller＇s noncavitation hydrodynamics and hydroacoustics, three similar 7bladed highly-skewed propellers in the wake flow are addressed with diameters of 250, 500 and 1 000 mm, respec...Regarding the scale effects on propeller＇s noncavitation hydrodynamics and hydroacoustics, three similar 7bladed highly-skewed propellers in the wake flow are addressed with diameters of 250, 500 and 1 000 mm, respectively. The discrete line-spectrum noise and its standardized spectrum level scaling law, together with the total sound pressure level are analyzed. The non-cavitation noise predictions are completed by both the frequency domain method and the time domain method. As a fluctuated noise source, the time-dependent fluctuated pressure and normal velocity distribution on propeller blades are obtained by the unsteady Reynolds-averaged Navier-Stokes （ URANS ） simulation. Results show that the pressure coefficient distribution of three propellers on the 0.7R section is nearly superposed under the same advance ratio. The periodic thrust fluctuation of three propellers can exactly reflect the tonal components of the axial passing frequency （APF） and the blade passing frequency （BPF）, and the fluctuation enhancement from the small to the middle propeller at the BPF is greater than that from the middle to the big one. By the two noise prediction methods, the increment of the total sound pressure level from the small to the big propeller differs by 2.49 dB. Following the standardized scaling law, the spectrum curves of the middle and big propellers are nearly the same while significantly differing from the small one. The increment of both the line-spectrum level and the total sound pressure increases with the increase in diameter. It is suggested that the model scale of the propeller should be as large as possible in engineering to reduce the prediction error of the empirical scalin~ law and weaken the scale effects.展开更多
The alteration of surface properties of chalcopyrite after biological conditioning with Leptospirillum ferriphilum was studied by adsorption,zeta-potential,contact angle and bioleaching tests.The strains of L.ferriphi...The alteration of surface properties of chalcopyrite after biological conditioning with Leptospirillum ferriphilum was studied by adsorption,zeta-potential,contact angle and bioleaching tests.The strains of L.ferriphilum cultured using different energy sources（either soluble ferrous ion or chalcopyrite） were used.The adhesion of bacteria to the chalcopyrite surface was a fast process.Additionally,the adsorption of substrate-grown bacteria was greater and faster than that of liquid-grown ones.The isoelectric point（IEP） of chalcopyrite moved toward that of pure L.ferriphilum after conditioning with bacteria.The chalcopyrite contact angle curves motioned diversely in the culture with or without energy source.The results of X-ray diffraction patterns（XRD）,scanning electron microscopy（SEM） and energy-dispersive X-ray spectroscopy（EDS） analysis indicate that the surface of chalcopyrite is covered with sulfur and jarosite during the bioleaching process by L.ferriphilum.Furthermore,EDS results imply that iron phase dissolves preferentially from chalcopyrite surface during bioleaching.The copper extraction is low,resulting from the formation of a passivation layer on the surface of chalcopyrite.The major component of the passivation layer that blocked continuous copper extraction is sulfur instead of jarosite.展开更多
A novel strip-coil-baffle structure used to enhance heat transfer and support the tube bundle for a tube-shell heat exchanger is proposed. The new structure can sleeve the tubes in bundle alternatively to create a vor...A novel strip-coil-baffle structure used to enhance heat transfer and support the tube bundle for a tube-shell heat exchanger is proposed. The new structure can sleeve the tubes in bundle alternatively to create a vortex flow in a heat exchanger. The numerical simulation on the flow and heat transfer characteristics for this new structure heat exchanger is conducted. The computational domain consists of two strip-coil sleeved tubes and two bare tubes oppositely placed at each comer of a square. The velocity and temperature fields in such strip-coil-baffled channel are simulated using FLUENT software. The effects of the strip-coil-baffles on heat transfer enhancement and flow resistance in relation to the Reynolds number are analyzed. The results show that this new structure bundle can enhance the heat transfer coefficient up to a range of 40% to 55% in comparison with a bare tube bundle; meanwhile, higher flow resistance is also accompanied. It is believe that the strip-coil- baffled heat exchanger should have promising applications in many industry fields.展开更多
文摘以钩端螺旋体基因组DNA为模板,通过酶联聚合反应(PCR)得到钩端螺旋体中prmA的同源基因lip-rmA的全基因编码序列,并克隆到原核表达载体pET22b中。通过优化大肠杆菌培养和诱导条件,含目的蛋白的融合蛋白可溶表达量达到40 mg/L,约占菌体总蛋白的40%。经Ni-NTA His Bind亲和柱纯化,得到纯度大于95%的目的蛋白。氨基酸序列同源性分析显示liPrmA与原核生物和真核生物的核糖体蛋白L11甲基化转移酶的功能域一级结构高度一致;活性分析显示,纯化的liPrmA有钩端螺旋体核糖体蛋白L11甲基化转移酶的活性。
基金The National Natural Science Foundation of China(No.51009144)
文摘Regarding the scale effects on propeller＇s noncavitation hydrodynamics and hydroacoustics, three similar 7bladed highly-skewed propellers in the wake flow are addressed with diameters of 250, 500 and 1 000 mm, respectively. The discrete line-spectrum noise and its standardized spectrum level scaling law, together with the total sound pressure level are analyzed. The non-cavitation noise predictions are completed by both the frequency domain method and the time domain method. As a fluctuated noise source, the time-dependent fluctuated pressure and normal velocity distribution on propeller blades are obtained by the unsteady Reynolds-averaged Navier-Stokes （ URANS ） simulation. Results show that the pressure coefficient distribution of three propellers on the 0.7R section is nearly superposed under the same advance ratio. The periodic thrust fluctuation of three propellers can exactly reflect the tonal components of the axial passing frequency （APF） and the blade passing frequency （BPF）, and the fluctuation enhancement from the small to the middle propeller at the BPF is greater than that from the middle to the big one. By the two noise prediction methods, the increment of the total sound pressure level from the small to the big propeller differs by 2.49 dB. Following the standardized scaling law, the spectrum curves of the middle and big propellers are nearly the same while significantly differing from the small one. The increment of both the line-spectrum level and the total sound pressure increases with the increase in diameter. It is suggested that the model scale of the propeller should be as large as possible in engineering to reduce the prediction error of the empirical scalin~ law and weaken the scale effects.
基金Project (2010CB630903) supported by the National Basic Research Program of China
文摘The alteration of surface properties of chalcopyrite after biological conditioning with Leptospirillum ferriphilum was studied by adsorption,zeta-potential,contact angle and bioleaching tests.The strains of L.ferriphilum cultured using different energy sources（either soluble ferrous ion or chalcopyrite） were used.The adhesion of bacteria to the chalcopyrite surface was a fast process.Additionally,the adsorption of substrate-grown bacteria was greater and faster than that of liquid-grown ones.The isoelectric point（IEP） of chalcopyrite moved toward that of pure L.ferriphilum after conditioning with bacteria.The chalcopyrite contact angle curves motioned diversely in the culture with or without energy source.The results of X-ray diffraction patterns（XRD）,scanning electron microscopy（SEM） and energy-dispersive X-ray spectroscopy（EDS） analysis indicate that the surface of chalcopyrite is covered with sulfur and jarosite during the bioleaching process by L.ferriphilum.Furthermore,EDS results imply that iron phase dissolves preferentially from chalcopyrite surface during bioleaching.The copper extraction is low,resulting from the formation of a passivation layer on the surface of chalcopyrite.The major component of the passivation layer that blocked continuous copper extraction is sulfur instead of jarosite.
基金The National Basic Research Program of China(973Program) (NoG2000026303)the National Natural Science Foun-dation of China (No50176008)
文摘A novel strip-coil-baffle structure used to enhance heat transfer and support the tube bundle for a tube-shell heat exchanger is proposed. The new structure can sleeve the tubes in bundle alternatively to create a vortex flow in a heat exchanger. The numerical simulation on the flow and heat transfer characteristics for this new structure heat exchanger is conducted. The computational domain consists of two strip-coil sleeved tubes and two bare tubes oppositely placed at each comer of a square. The velocity and temperature fields in such strip-coil-baffled channel are simulated using FLUENT software. The effects of the strip-coil-baffles on heat transfer enhancement and flow resistance in relation to the Reynolds number are analyzed. The results show that this new structure bundle can enhance the heat transfer coefficient up to a range of 40% to 55% in comparison with a bare tube bundle; meanwhile, higher flow resistance is also accompanied. It is believe that the strip-coil- baffled heat exchanger should have promising applications in many industry fields.
