The characteristics of a new type of drainage channel with staggered indented sills for controlling debris flows were studied. The intermediate fluid in the non-viscous debris flow exhibited a helical movement, wherea...The characteristics of a new type of drainage channel with staggered indented sills for controlling debris flows were studied. The intermediate fluid in the non-viscous debris flow exhibited a helical movement, whereas the fluid near the sidewall had a stop-start movement pattern; the viscous debris flow exhibited a stable structure between the indented sills. The experimental results indicate that the mean velocity of the debris flow increased with increasing channel gradients, and the debris flow velocity was slightly affected by the angle of the sills. The average velocity of the non-viscous debris flow increased in the range of(0.5–1.5) interval between the indented sills, whereas the average velocity of the viscous debris flow increased initially and then decreased in the range of(0.75–1.25) interval between the indented sills. The depth of the non-viscous debris flow tended to gradually increase as the channel gradients increased, whereas the depth of the viscous debris flow gradually decreased as the channel gradients increased. When the discharge of the debris flow was constant, the angle and the interval between the indented sills had a slight effect on the depth of the viscous debris flow, whereas the depth of the non-viscous debris flow exhibited a different trend, as the sill angles and intervals were varied.展开更多
The Gpd gene was amplified from the genomic DNA of Treponema the appropriate site of pcDNA3.1 （ ＋ ） vector. The expression of pcDNA3. I was tested with Western blotting and technology of immunoeytochemisty. New mun...The Gpd gene was amplified from the genomic DNA of Treponema the appropriate site of pcDNA3.1 （ ＋ ） vector. The expression of pcDNA3. I was tested with Western blotting and technology of immunoeytochemisty. New munized with the eukaryotic expression recombinant pcDNA3, 1 （ ＋ ）-Gpd pallidum and cloned into （ ＋ ）-Gpd in Hel,a cells Zealand rabbits were imA fusion protein of C, pd with 4.1 kDa has been effectively expressed in HeLa cells, which were detected bv Western blotting and the immunocytochemistry techniques. The New Zealand rabbits were able to elicit the specific antibody after immunization with the nucleic acid vaccine. The antibody titer could reach as high as 1 ： 1024 after 2 weeks of the third injection; and the splenocytes proliferated evidently due to the Gpd protein stimulation. Both the antibody titer and the splenocytes proliferation were higher substantially than those of controls （ P 〈 0.01 ）. All above data will contribute to an experimental basis of further study of the biological function of Gpd protein as well as DNA vaccine for syphilis.展开更多
基金sponsored by the Key Deployment Project of Chinese Academy of Sciences(Grant No.KZZD-EW-05-01)the National Science Foundation of China(Grant No.41072270)
文摘The characteristics of a new type of drainage channel with staggered indented sills for controlling debris flows were studied. The intermediate fluid in the non-viscous debris flow exhibited a helical movement, whereas the fluid near the sidewall had a stop-start movement pattern; the viscous debris flow exhibited a stable structure between the indented sills. The experimental results indicate that the mean velocity of the debris flow increased with increasing channel gradients, and the debris flow velocity was slightly affected by the angle of the sills. The average velocity of the non-viscous debris flow increased in the range of(0.5–1.5) interval between the indented sills, whereas the average velocity of the viscous debris flow increased initially and then decreased in the range of(0.75–1.25) interval between the indented sills. The depth of the non-viscous debris flow tended to gradually increase as the channel gradients increased, whereas the depth of the viscous debris flow gradually decreased as the channel gradients increased. When the discharge of the debris flow was constant, the angle and the interval between the indented sills had a slight effect on the depth of the viscous debris flow, whereas the depth of the non-viscous debris flow exhibited a different trend, as the sill angles and intervals were varied.
文摘The Gpd gene was amplified from the genomic DNA of Treponema the appropriate site of pcDNA3.1 （ ＋ ） vector. The expression of pcDNA3. I was tested with Western blotting and technology of immunoeytochemisty. New munized with the eukaryotic expression recombinant pcDNA3, 1 （ ＋ ）-Gpd pallidum and cloned into （ ＋ ）-Gpd in Hel,a cells Zealand rabbits were imA fusion protein of C, pd with 4.1 kDa has been effectively expressed in HeLa cells, which were detected bv Western blotting and the immunocytochemistry techniques. The New Zealand rabbits were able to elicit the specific antibody after immunization with the nucleic acid vaccine. The antibody titer could reach as high as 1 ： 1024 after 2 weeks of the third injection; and the splenocytes proliferated evidently due to the Gpd protein stimulation. Both the antibody titer and the splenocytes proliferation were higher substantially than those of controls （ P 〈 0.01 ）. All above data will contribute to an experimental basis of further study of the biological function of Gpd protein as well as DNA vaccine for syphilis.
