针刺对自发性高血压大鼠血压和抗氧化酶SOD、GSH-PX的影响

目的：观察针刺对自发性高血压大鼠（SHR）血压的影响,从抗氧化酶角度探讨针刺降压机制。方法：将SHR随机分为针刺组、模型组,以京都Wistar大鼠（WKY）作为血压正常的对照组。针刺组取双侧人迎穴,施以捻转手法,捻转频率为120~160次/min,行针1min,每日针刺1次;模型组和正常组进行与针刺组相同时间、相同程度的捉抓。每天针刺前后测量收缩压和舒张压。针刺20d后,取胸主动脉,用黄嘌呤氧化酶比色法测定SOD活性,化学比色法测定GSH-PX活性。结果：针刺20d后SHR收缩压和舒张压均较针刺前降低,且均低于模型组。针刺组SOD和GSH-PX活性较模型组均有升高的趋势。结论：针刺可降低SHR血压,其机制可能与提高SOD和GSH-PX活性,从而提高机体抗氧化能力有关。

针刺降压的生理自律性探讨

按病情将高血压病分为3阶段,即校正、稳定、巩固期,采用按压、点刺、叩刺法联合治疗50例,有效率90%,其中约10%患者停止针刺治疗后,出现血压自然下降现象。一疗程后的第3个月、第6个月,各行2周巩固治疗,经3年随访,无复发患者。另有患者偶遇精神刺激、疲劳紧张等诱因,出现血压轻微升高,但为时很短,1天内即可恢复正常。表明经针刺调整的血压巩固后,将长期稳定在正常准位上,已成为生理自律性,虽有暂时波动升高,亦会自然回归正常。进而表明按压、点刺、叩刺治疗高血压病有催化、塑型、固定之作用。

陈独秀流寓江津的日子

今年５月４日，是伟大的五四运动８０周年纪念日。被毛泽东同志誉为“五四运动时期的总司令”的陈独秀，晚年流寓江津，蛰居鹤山坪石墙院，直至走到生命的尽头。作为《新青年》杂志主编、中共中央第一任至第五任总书记的陈独秀，晚年生活境况如何？春暖花开时节，笔者专程...

Effects of Magnetic Needle Acupuncture on Blood Pressure and Plasma ET-1 Level in the Patient of Hypertension

Endothelin-1 (ET-1) is ever known as a bio-peptidewith the strongest vasoconstrictive action and withwide biological effects.

The Effects of Acupuncture on Blood Pressure in Different Patients

Eighty-seven patients with different types of diseases were treated with acupuncture,and the changes of their blood pressure were investigated.The results showed that the total level of systolic pressure in the 87 cases decreased (P<0.05).Especially,the systolic pressure in patients accompanied with hypertension decreased significantly.It is suggested that when acupuncture is adopted to treat other diseases,it can decrease the systolic pressure to a certain extent in patients accompanied with hypertension,but it does not exert significant influence on both systolic and diastolic pressure in patients with normal blood pressure.

EFFECT OF ACUPUNCTURE OF FENGCHI (GB 20) ON BLOOD PRESSURE AND SERUM IL-6 and PLASMA ET LEVELS IN PATIENTS WITH HYPERTENSION

Objective：To observe the effect of acupuncture of Fengchi （风池 GB 20） on blood pressure, serum IL-6 and plasma endothelin （ET） contents in patients with hypertension. Methods： Thirty cases of hy-pertension patients were made up of treatment group and treated with acupuncture of Fengchi （GB 20） ; while 20 healthy subjects were made up of control group. Blood pressure was determined before and after the treatment. Fasting blood samples were taken from the cubital venous vessel for detecting serum interleukin （IL）-6 and plasma ET contents with radioimmunoassay before and after acupuncture treatment. Results： Before the treatment, in hypertension patients, serum IL-6 and plasma ET levels were significant higher thanthose in healthy subjects （P〈 0.01 ）; while after the treatment, blood pressure of treatment group declined significantly （ P 〈 0.01 ）, serum IL-6 and plasma ET levels decreased considerably （ P 〈 0.01 ）. Conclusion： Acupuncture of Fengchi （GB 20） can lower blood pressure, regulate cellular immunity and secretion functions of the vascular endothelial cells in hypertension patients.

EFFECTS OF ACUPUNCTURE AT FENGCHI POINT (GB 20) ON CEREBRAL BLOOD FLOW

Blood velocity in the vertebral artery and the basilar artery was observed before and after acupuncture at Fengchi point (GB 20) in 97 patients by transcranial Doppler ultrasonic detecting. The results showed that the blood velocity in patients with either high or low blood flow had significant changes after acupuncture (P

Effect of electroacupuncture at Qūchí (曲池 LI 11) and Tàichōng (太冲 LR 3) on blood pressure variability in young patients with hypertension

Objective To compare therapeutic effects of electroacupuncture and western medicine on blood pressure variability in young patients with hypertension. Methods Sixty cases of young patients of hypertension were randomly divided into an electroacupuncture group and a western medicine group, 30 cases in each group. In the electroacupuncture group, electroacupuncture was applied at Quchi （曲池LI 11） and Taichong （太冲LR 3）. In the western medicine group, Captopril was taken orally. Twenty-four hour dynamic blood pressure, and blood pressure variability and circadian rhythms were observed after treatment for 14 days in the two groups. Results Electroacupuncture at Quchi （曲池LI 11） and Taichong （太冲LR 3） significantly decreased systolic pressure and diastolic pressure at different time phases in the young patients with hypertension （all P〈0.01）, with no significant difference as compared with the western medicine group （all P〉0.05）; electroacupuncture at Quchi （曲池LI 11） and Taichong （太冲LR 3） significantly reduced the standard deviations of systolic pressure and diastolic pressure at different time phases in the young patients with hypertension as compared with those before the treatment （P〈0.01, P〈0.05）. There were statistically significant differences between the two groups in 24 h systolic standard deviation （24 h SSD）, 24 h diastolic standard deviation （24 h DSD）, daytime SSD （dSSD）, nighttime SSD （nSSD） and nighttime DSD （nDSD） （P〈0.01, P〈0.05）; and there was significant difference between the two groups in numbers of the Dipper and the non-Dipper after treatment （P〈0.05）. Conclusion Electroacupuncture at Quchi （曲池LI 11） and Taichong （太冲LR 3） has a better long-term effect of decreasing blood pressure and improves effectively changes of circadian rhythms in young patients with hypertension, which are an effective point pair for hypertension.

Clinical Research of Different Syndromes of Hypertension Treated by Acupuncture

Primary hypertension is the most common cardiovascular disease. Some relevant research indicates that puncturing Fengchi （GB 20） can effectively reduce the elevated blood pressure of patient with hypertension. However, is this method effective for all the patients with hypertension？ With regards to this, the author conducted a therapeutic observation in clinic, puncturing Fengchi （GB 20） according to different syndrome of patient with hypertension. Now the conclusion is as follows.

Effect of acupuncture at Renying(ST 9) on gene expression profile of hypothalamus in spontaneously hypertensive rats

OBJECTIVE: To investigate changes in gene expression profiles in the hypothalamus related to the effects of acupuncture at the Renying(ST 9) acupoint in spontaneously hypertensive(SH) rats.METHODS: We randomly divided 18 SH rats into Renying(ST 9) group and model control group, 9 body weight-matched Wistar-Kyoto rats were used as blank controls. Acupuncture was performed manually for 20-min daily over 28 d in the Renying(ST 9) group. Rat Gene 2.0 array technology was used for the determination of gene expression profiles and the screened key genes were verified by real-time quantitative polymerase chain reaction(RT-PCR) analyses.RESULTS: The different groups exhibited differential gene expression: compared with the blank control group, 48 genes were up-regulated and 91 genes were down-regulated in the model group;compared with the model group, 79 genes were up-regulated and 80 genes were down-regulated in Renying(ST 9) group. The RT-PCR results of the key genes including Chi3 l1, Ephx2, Klk1, 5-HT1 A and Cbs were consistent with that of gene chip analysis.CONCLUTION: Acupuncture at Renying(ST 9)could significantly lower the blood pressure of SH rats and affect their hypothalamic gene expression profile. Genes associated with the contraction of vascular smooth muscle and the regulation of inflammation, neurotransmitters may be involved in acupuncture's antihypertensive mechanism.

Peripheral Nerve Discharge Elicited by Manual Acupuncture at Zusanli (ST 36) Regulates Blood Pressure in Anesthetized Rats

Objective： To investigate target organ response by recording mean arterial blood pressure （MAP） fluctuation corresponding to nerve-tract discharges from the nerve innervating acupoint of Zusanli （ST 36） in the hind limb evoked by MA in anesthetized rats. Methods： Male SD rats anesthetized with chloral hydrate were randomly divided into 3 groups which were treated with manual acupuncture （MA）, injection of lidocaine followed by MA and injection of normal saline （NS） followed by MA, respectively. The right carotid artery was canulated for persistent measurement of the blood pressure and meanwhile nerve discharges from the nerve-tract were recorded for analysis with amplitude spike counts for every 5 s. Results： The results showed that there were significant nerve discharges recorded from the nerve-tract when applying MA at Zusanli （ST 36） and simultaneous decrease in the MAP, while there was no response when inserting a needle into the Zusanli （ST 36） without manipulation （P〈0.05）. Furthermore, the reduction of MAP during MA could be completely abolished after blockade of peripheral nerve discharges with an injection of lidocaine into the tissue around Zusanli （ST 36） but not with that of normal saline （NS）. Conclusion： These results indicate that MA at Zusanli （ST 36） can elicit the peripheral nerve discharges from the nerve innervating the acupoint; such kind of nerve discharges may contain acupuncture signal regulating blood pressure via somato-cardiovascular reflex.

Relationship Between the Central Hypotensive Effect of Acupuncture or Melatonin and the Changes of Medullary Amino Acid Neurotransmitter

To investigate whether changes of amino acid neurotransmitter releases in the rostral ventrolateral medulla （rVLM） were related to acupuncture and to the antihypertensive effect of melatonin （Mel） microinjected into the anterior hypothalamic area （AHA） of rats with stress-induced hypertension （SIH）, as well as to compare and analyze the relationship between the both antihypertensive mechanisms of acupuncture and of Mel in the AHA. Methods： Animal model of SIHR was made by electric foot shocks combined with noises. Electroacupuncture （EA） was used and “Zusanli” acupoint of both side was selected. The technique of drug microinjection into the brain was used to observe the change of blood pressure （BP）, and synchronously, brain microdialysis was performed for collecting dialysate samples, and then the concentration of amino acid neurotransmitters in the dialysate samples was determined by high performance liquid chromatography combined with fluorescent detection （HPLC-FD）. Results： After the animal received stress treatment, the BP elevated, synchronously, the release of glutamate （Glu） in the rVLM increased, and when EA was performed, the elevated BP of the rats with SIH decreased, simultaneously, the release of Glu in the rVLM decreased also. After Mel was microinjected of into the AHA of the SIHR, the elevated BP attenuated, meanwhile, the release of Glu decreased, and those of γ-aminobutyric acid （GABA） and taurine （Tau） increased in the rVLM.Administration of bicuculline, an antagonist of GABAA receptor, into the rVLMprior to microinjection of Mel imo the AHA could partially block the depressor effect of Mel in the AHA. Conclusion： The decrease in the release of Glu in the rVLM contributes to the antihypertensive effect of both acupuncture and Mel in the AHA in the rats with SIH, and the increase in the release of GABA and Tau in the rVLM is also im0ortam to Mel in the AHA.

Mechanism of the Reversal Effect of Acupuncture on the Fall of Blood Pressure Induced by Angiotensin-(1-7)in the Caudal Ventrolateral Medulla of Rats

Objective：To investigate the mechanism of the reversal effect of acupuncture on the fall of blood pressure induced by angiotensin （1-7）[Ang-（1 7）] in the caudal ventrolateral medulla （CVLM） of rats. Methods： Ang-（1-7） and its selective receptor antagonist （DAla7）, Ang（1-7） and Ang（779） were microinjected into the CVLM respectively to induce the change of blood pressure, during which, the concentrations of amino acid neurotransmitters were detected by means of microdialysis and high performance liquid chromatography （HPLC）combined with fluorescent detector, on the other hand, the effects of electro-acupuncture （EA） on the level of blood pressure and the concentrations of amino acid neurotransmitters were also observed. Results： Unilateral microinjection of Ang（1 7） into the CVLM could decrease the blood pressure （BP）, which was accompanied by an increase in the release of Glu and a decrease in the release of Tau in the same site; Whereas microinjection of Ang-779 into the CVLM could elevate BP, which was accompanied by a decrease in the release of Glu and an increase in the release of Tau; EA at acupoint Zusanli （ST 36） for 20 min could inhibit the fall of blood pressure induced by microinjection of Ang-（1-7） into the CVLM and the elevation of blood pressure induced by microinjection of Ang-779 into the CVLM, and in the meantime, it could inhibit the changes of the release of Glu and Tau induced by microinjection of Ang-（1-7） and Ang-779 into the CVLM. Conclusion： The reversal effect of EA on the fall or elevation of blood pressure induced by microinjection of Ang-（1-7） or Ang-779 into the CVLM might be related to the changes of Glu and Tau release.