本文采用平板式电容耦合射频(RF,13.56MHz)等离子体源,以乙二醇二甲基醚(Ethylene Glycol Di Methyl Ether)为聚合单体,氩气为辅助气体,在连续与脉冲射频等离子体两种放电模式下合成类聚乙烯氧(PEO-like)功能聚合薄膜。实验研究了等离...本文采用平板式电容耦合射频(RF,13.56MHz)等离子体源,以乙二醇二甲基醚(Ethylene Glycol Di Methyl Ether)为聚合单体,氩气为辅助气体,在连续与脉冲射频等离子体两种放电模式下合成类聚乙烯氧(PEO-like)功能聚合薄膜。实验研究了等离子体放电参数:等离子体放电功率、工作气压、放电模式(连续或脉冲)和聚合时间等对聚合物表面结构、功能团含量、表面成分性能以及和血小板吸附等影响。利用接触角测定仪(WCA)、傅里叶变换红外光谱(FTIR)、原子力显微镜(AFM)等手段对聚合薄膜的结构、成分和形貌进行细致的分析。同时本文还进行体外细胞培养法,研究了类PEO功能薄膜对富血血小板的吸附,通过倒置显微镜观察细胞黏附的数量和形态变化。得到的结论为:采用RF-PECVD可以在较小功率的连续等离子体放电模式,或较长脉冲间隔的脉冲放电模式下得到结构稳定的PEO生物功能薄膜,所制备的PEO生物功能薄膜具有良好的抗血小板吸附性能。展开更多
A synthetic diblock copolymer poly（2-ethyl-2-oxazoline）-poly（D,L-lactide） （PEOz-PLA） can self-assemble into micelles with an increased efficiency of drug delivery. However, the interactions of blood-micelles and...A synthetic diblock copolymer poly（2-ethyl-2-oxazoline）-poly（D,L-lactide） （PEOz-PLA） can self-assemble into micelles with an increased efficiency of drug delivery. However, the interactions of blood-micelles and cell-micelles remain unclear. In the present study, we aimed to assess the hemocompatibility and cytocompatibility of PEOz-PLA micelles in order to clarify its potentials as carriers for drug delivery. Blood compatibility of the micelles was evaluated by hemolysis analysis, coagulation test, platelet activation investigation and assessment of their interaction with protein. The results revealed that PEOz-PLA micelles had a favorable blood compatibility. In addition, PEOz-PLA micelles showed a good cytocompatibility through SRB assay, presenting only negligible cytotoxicity when incubated with KBv cells. Taken together, PEOz-PLA micelles could be used as a hemocompatible and cytocompatible drug carrier for intravenous administration.展开更多
Objective： To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility. Methods： The effects of...Objective： To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility. Methods： The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor （VEGF） expression of ECV304 cells, and VEGF, angiopoietin-1 （Ang-1）, platelet-derived growth factor （PDGF） and fibroblast growth factor 2 （FGF2） expression of WI-38 cells were assessed by 3-（4,5）-dimethylthiahiazo （-z-yl）-3,5-di-phenytetrazoliumromide （MTT） assay, viable cell counting, flow cytometry （FCM）, and enzyme-linked immunosorbant assay （ELISA）. Results： We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells. Conclusion： The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.展开更多
文摘本文采用平板式电容耦合射频(RF,13.56MHz)等离子体源,以乙二醇二甲基醚(Ethylene Glycol Di Methyl Ether)为聚合单体,氩气为辅助气体,在连续与脉冲射频等离子体两种放电模式下合成类聚乙烯氧(PEO-like)功能聚合薄膜。实验研究了等离子体放电参数:等离子体放电功率、工作气压、放电模式(连续或脉冲)和聚合时间等对聚合物表面结构、功能团含量、表面成分性能以及和血小板吸附等影响。利用接触角测定仪(WCA)、傅里叶变换红外光谱(FTIR)、原子力显微镜(AFM)等手段对聚合薄膜的结构、成分和形貌进行细致的分析。同时本文还进行体外细胞培养法,研究了类PEO功能薄膜对富血血小板的吸附,通过倒置显微镜观察细胞黏附的数量和形态变化。得到的结论为:采用RF-PECVD可以在较小功率的连续等离子体放电模式,或较长脉冲间隔的脉冲放电模式下得到结构稳定的PEO生物功能薄膜,所制备的PEO生物功能薄膜具有良好的抗血小板吸附性能。
基金National Natural Science Foundation of China(Grant No.81172990)the National Key Science Research Program of China(Grant No.973 Program,2009CB930300)+1 种基金Innovation Team of Ministry of Education(Grant No.BMU20110263)the Open Project Program of State Key Laboratory of Drug Delivery Technology and Pharmacokinetics,Tianjin Institute of Pharmaceutical Research
文摘A synthetic diblock copolymer poly（2-ethyl-2-oxazoline）-poly（D,L-lactide） （PEOz-PLA） can self-assemble into micelles with an increased efficiency of drug delivery. However, the interactions of blood-micelles and cell-micelles remain unclear. In the present study, we aimed to assess the hemocompatibility and cytocompatibility of PEOz-PLA micelles in order to clarify its potentials as carriers for drug delivery. Blood compatibility of the micelles was evaluated by hemolysis analysis, coagulation test, platelet activation investigation and assessment of their interaction with protein. The results revealed that PEOz-PLA micelles had a favorable blood compatibility. In addition, PEOz-PLA micelles showed a good cytocompatibility through SRB assay, presenting only negligible cytotoxicity when incubated with KBv cells. Taken together, PEOz-PLA micelles could be used as a hemocompatible and cytocompatible drug carrier for intravenous administration.
基金supported by the National Basic Research Program (973) of China (No.2005CB623906)the Medical Development Foundation of Soochow University (No.EE134702),China
文摘Objective： To explore the feasibility of using regenerated silk fibroin membrane to construct artificial skin substitutes for wound healing, it is necessary to evaluate its cytocompatibility. Methods： The effects of regenerated silk fibroin film on cytotoxicity, adhesion, cell cycle, and apoptosis of L929 cells, growth and vascular endothelial growth factor （VEGF） expression of ECV304 cells, and VEGF, angiopoietin-1 （Ang-1）, platelet-derived growth factor （PDGF） and fibroblast growth factor 2 （FGF2） expression of WI-38 cells were assessed by 3-（4,5）-dimethylthiahiazo （-z-yl）-3,5-di-phenytetrazoliumromide （MTT） assay, viable cell counting, flow cytometry （FCM）, and enzyme-linked immunosorbant assay （ELISA）. Results： We showed that the regenerated silk fibroin film was not cytotoxic to L929 cells and had no adverse influence on their adhesion, cell cycle or apoptosis; it had no adverse influence on the growth and VEGF secretion of ECV304 cells and no effect on the secretion of VEGF, Ang-1, PDGF and FGF2 by WI-38 cells. Conclusion： The regenerated silk fibroin film should be an excellent biomaterial with good cytocompatibility, providing a framework for reparation after trauma in clinical applications.
