Salvia miltiorrhiza （Sm） is a traditional herbal medicine with multiple effects on various diseases. Its water-soluble parts have been used to produce injectional powder. In this study, liver fibrosis rats were indu...Salvia miltiorrhiza （Sm） is a traditional herbal medicine with multiple effects on various diseases. Its water-soluble parts have been used to produce injectional powder. In this study, liver fibrosis rats were induced by intraperitoneal injection of dimethylnitrosarnine for 3 consecutive days per week for 4 weeks. After 2 weeks, rats in the positive drug group were subcutaneously injected with 8×10^5 IU/kg IFNα2b, while the Sm treatment groups were intraperitoneally injected with 50, 100 and 200 mg/kg solution of Sm injectional powder, respectively, for 6 days per week for 4 weeks. The results showed that either IFNα2b or the Sm injectional powder significantly increased the body weight and liver to spleen ratio, and three doses of the powder brought down the spleen index. Serum analysis showed that both IFNα2b and the Sm powder reduced levels of alanine transaminase and total bilirubin, while only 100 and 200 mg/kg of the Sm powder ameliorated aspartate transaminase and albumin levels. In the collagen examination, reduced hyaluronic acid and procollagen type III levels, less fibrous hyperplasia and collagen deposits, and improved hepatocyte states were clearly observed in rats treated with either IFNα2b or Sm injectional powder. In addition, the mechanism of action of the Sm powder was also studied. Immunohistochemical staining showed that IFNα2b and Sm injectional powder significantly down-regulated the expression of transforming growth factor-β1 （TGF-β1） and platelet derived growth factor （PDGF）. In conclusion, Sm injectional powder has protective effects on dimethylnitrosamine-initiated liver fibrosis in rats, and the mechanism may include the down-regulation of TGF-β1 and PDGF.展开更多
Objective To observe the efficacy of mild moxibustion combined with arthroscopic meniscal repair in the treatment of meniscal injury and to explore its action mechanism.Methods Ninety-eight patients with meniscal inju...Objective To observe the efficacy of mild moxibustion combined with arthroscopic meniscal repair in the treatment of meniscal injury and to explore its action mechanism.Methods Ninety-eight patients with meniscal injury were divided into a surgery group and a moxibustion plus surgery group by the random number table method,with 49 cases in each group.Both groups received arthroscopic meniscal repair,and the moxibustion plus surgery group was treated with add-on mild moxibustion.The Lysholm score,visual analog scale(VAS)score,and mobility of the affected knee were measured before and after treatment,and transforming growth factor(TGF)-β1 and platelet-derived growth factor(PDGF)levels were measured in the fluid of the affected knee joint.The healing of the meniscus was recorded at a follow-up visit 3 months after the surgery.Results After treatment,the Lysholm score increased,the VAS score decreased in both groups,and the intra-group differences in both groups were statistically significant(P<0.05).The Lysholm score was higher in the moxibustion plus surgery group than in the surgery group,and the VAS score was lower in the moxibustion plus surgery group than in the surgery group.The differences between groups in both scores were statistically significant(P<0.05).The mobility of the affected knee joint increased in both groups(P<0.05),and it was greater in the moxibustion plus surgery group than in the surgery group(P<0.05).When compared with pretreatment,the levels of TGF-β1 and PDGF in the fluid of the knee joint increased in both groups(P<0.05),and the levels of TGF-β1 and PDGF in the fluid of the affected knee joint were higher in the moxibustion plus surgery group than in the surgery group(P<0.05).The healing of the meniscus in the moxibustion plus surgery group was significantly better than that in the surgery group at the follow-up visit 3 months after the surgery(P<0.05).Conclusion The effect of mild moxibustion combined with arthroscopic meniscal repair is better than the surgery alone in improving knee function,relieving pain,increasing mobility of the affected knee,and promoting meniscal healing,which may be related to the up-regulation of TGF-β1 and PDGF levels in the fluid of knee joint.展开更多
文摘Salvia miltiorrhiza （Sm） is a traditional herbal medicine with multiple effects on various diseases. Its water-soluble parts have been used to produce injectional powder. In this study, liver fibrosis rats were induced by intraperitoneal injection of dimethylnitrosarnine for 3 consecutive days per week for 4 weeks. After 2 weeks, rats in the positive drug group were subcutaneously injected with 8×10^5 IU/kg IFNα2b, while the Sm treatment groups were intraperitoneally injected with 50, 100 and 200 mg/kg solution of Sm injectional powder, respectively, for 6 days per week for 4 weeks. The results showed that either IFNα2b or the Sm injectional powder significantly increased the body weight and liver to spleen ratio, and three doses of the powder brought down the spleen index. Serum analysis showed that both IFNα2b and the Sm powder reduced levels of alanine transaminase and total bilirubin, while only 100 and 200 mg/kg of the Sm powder ameliorated aspartate transaminase and albumin levels. In the collagen examination, reduced hyaluronic acid and procollagen type III levels, less fibrous hyperplasia and collagen deposits, and improved hepatocyte states were clearly observed in rats treated with either IFNα2b or Sm injectional powder. In addition, the mechanism of action of the Sm powder was also studied. Immunohistochemical staining showed that IFNα2b and Sm injectional powder significantly down-regulated the expression of transforming growth factor-β1 （TGF-β1） and platelet derived growth factor （PDGF）. In conclusion, Sm injectional powder has protective effects on dimethylnitrosamine-initiated liver fibrosis in rats, and the mechanism may include the down-regulation of TGF-β1 and PDGF.
文摘Objective To observe the efficacy of mild moxibustion combined with arthroscopic meniscal repair in the treatment of meniscal injury and to explore its action mechanism.Methods Ninety-eight patients with meniscal injury were divided into a surgery group and a moxibustion plus surgery group by the random number table method,with 49 cases in each group.Both groups received arthroscopic meniscal repair,and the moxibustion plus surgery group was treated with add-on mild moxibustion.The Lysholm score,visual analog scale(VAS)score,and mobility of the affected knee were measured before and after treatment,and transforming growth factor(TGF)-β1 and platelet-derived growth factor(PDGF)levels were measured in the fluid of the affected knee joint.The healing of the meniscus was recorded at a follow-up visit 3 months after the surgery.Results After treatment,the Lysholm score increased,the VAS score decreased in both groups,and the intra-group differences in both groups were statistically significant(P<0.05).The Lysholm score was higher in the moxibustion plus surgery group than in the surgery group,and the VAS score was lower in the moxibustion plus surgery group than in the surgery group.The differences between groups in both scores were statistically significant(P<0.05).The mobility of the affected knee joint increased in both groups(P<0.05),and it was greater in the moxibustion plus surgery group than in the surgery group(P<0.05).When compared with pretreatment,the levels of TGF-β1 and PDGF in the fluid of the knee joint increased in both groups(P<0.05),and the levels of TGF-β1 and PDGF in the fluid of the affected knee joint were higher in the moxibustion plus surgery group than in the surgery group(P<0.05).The healing of the meniscus in the moxibustion plus surgery group was significantly better than that in the surgery group at the follow-up visit 3 months after the surgery(P<0.05).Conclusion The effect of mild moxibustion combined with arthroscopic meniscal repair is better than the surgery alone in improving knee function,relieving pain,increasing mobility of the affected knee,and promoting meniscal healing,which may be related to the up-regulation of TGF-β1 and PDGF levels in the fluid of knee joint.
