As migraine is the result of an inflammatory mechanism with serotonergic sign alling, leucocyte function, platelet function and intercellular communication be tween those cells is likely to be connected to the final p...As migraine is the result of an inflammatory mechanism with serotonergic sign alling, leucocyte function, platelet function and intercellular communication be tween those cells is likely to be connected to the final pathway of the disease. We examined P- selectin expression on platelets (platelet activation) and leuc ocyte- platelet aggregate formation in 72 migraine patients during their attack - free interval and controls using a flow cytometric assay. Patients suffering from migraine without aura had a significantly increased platelet activation and leucocyte- platelet aggregation compared with the control group, unlike the mi graine patients with aura. Patients who had taken a triptan within 3 days prior to the investigation showed platelet activation values similar to the control gr oup. The variations in platelet activation patterns of migraine subgroups could indicate different pathomechanisms. Even outside an attack, migraine patients, p articularly those without aura, show an increased level of platelet activation w hich seems to be down- regulated by triptans. This mechanism may account for th e triptan- induced increases in headache frequency. The involvement of proinfla mmatory platelet- leucocyte cross- talk suggests a possible therapeutic strate gy using anti- inflammatory drugs.展开更多
目的探讨富白细胞-血小板纤维蛋白(leukocyte-and platelet-rich fibrin,L-PRF)作为盖髓剂对大鼠牙髓炎症调节的作用。方法脂多糖(LPS)构建牙髓炎大鼠模型,30只大鼠随机分成5组,即正常对照组、LPS造模组、iRoot BP PLUS组、L-PRF组、L-P...目的探讨富白细胞-血小板纤维蛋白(leukocyte-and platelet-rich fibrin,L-PRF)作为盖髓剂对大鼠牙髓炎症调节的作用。方法脂多糖(LPS)构建牙髓炎大鼠模型,30只大鼠随机分成5组,即正常对照组、LPS造模组、iRoot BP PLUS组、L-PRF组、L-PRF+iRoot BP PLUS组。直接盖髓14 d后取标本行显微CT(Micro-CT)扫描、苏木精-伊红(HE)及Masson染色、定量实时聚合酶链式反应(qRT-PCR)检测,观察牙髓炎症及硬组织形成程度。结果Micro-CT示:LPS组未见硬组织形成,L-PRF组冠髓内可见斑块状钙化物,BP组及联合组在穿髓孔处见较完整的钙化桥。组织学染色示:LPS组炎症最重、无硬组织形成;联合组炎症最轻,硬组织形成最完整、连续,两组差异有统计学意义(P<0.01);BP组与联合组的炎症及硬组织形成程度均差异无统计学意义(P>0.05)。qRT-PCR结果示:各实验组牙髓组织中TNF-α、IL-1β、IL-6 mRNA相对表达水平相较于LPS组均有所降低,但L-PRF组与LPS组的差异无统计学意义(P>0.05)。结论L-PRF联合iRoot BP PLUS作为复合盖髓材料,可用于大鼠牙髓炎牙髓组织修复以及牙髓炎症调节。展开更多
文摘As migraine is the result of an inflammatory mechanism with serotonergic sign alling, leucocyte function, platelet function and intercellular communication be tween those cells is likely to be connected to the final pathway of the disease. We examined P- selectin expression on platelets (platelet activation) and leuc ocyte- platelet aggregate formation in 72 migraine patients during their attack - free interval and controls using a flow cytometric assay. Patients suffering from migraine without aura had a significantly increased platelet activation and leucocyte- platelet aggregation compared with the control group, unlike the mi graine patients with aura. Patients who had taken a triptan within 3 days prior to the investigation showed platelet activation values similar to the control gr oup. The variations in platelet activation patterns of migraine subgroups could indicate different pathomechanisms. Even outside an attack, migraine patients, p articularly those without aura, show an increased level of platelet activation w hich seems to be down- regulated by triptans. This mechanism may account for th e triptan- induced increases in headache frequency. The involvement of proinfla mmatory platelet- leucocyte cross- talk suggests a possible therapeutic strate gy using anti- inflammatory drugs.
文摘目的探讨富白细胞-血小板纤维蛋白(leukocyte-and platelet-rich fibrin,L-PRF)作为盖髓剂对大鼠牙髓炎症调节的作用。方法脂多糖(LPS)构建牙髓炎大鼠模型,30只大鼠随机分成5组,即正常对照组、LPS造模组、iRoot BP PLUS组、L-PRF组、L-PRF+iRoot BP PLUS组。直接盖髓14 d后取标本行显微CT(Micro-CT)扫描、苏木精-伊红(HE)及Masson染色、定量实时聚合酶链式反应(qRT-PCR)检测,观察牙髓炎症及硬组织形成程度。结果Micro-CT示:LPS组未见硬组织形成,L-PRF组冠髓内可见斑块状钙化物,BP组及联合组在穿髓孔处见较完整的钙化桥。组织学染色示:LPS组炎症最重、无硬组织形成;联合组炎症最轻,硬组织形成最完整、连续,两组差异有统计学意义(P<0.01);BP组与联合组的炎症及硬组织形成程度均差异无统计学意义(P>0.05)。qRT-PCR结果示:各实验组牙髓组织中TNF-α、IL-1β、IL-6 mRNA相对表达水平相较于LPS组均有所降低,但L-PRF组与LPS组的差异无统计学意义(P>0.05)。结论L-PRF联合iRoot BP PLUS作为复合盖髓材料,可用于大鼠牙髓炎牙髓组织修复以及牙髓炎症调节。
