薄层色谱扫描法测定家兔血尿中硝基安定含量的研究

应用薄层色谱扫描法建立了家兔血尿中硝基安定的测定方法。通过对家兔血尿中硝基安定测定研究表明，服药２小时血药浓度达峰时，尿中硝基安定含量已相当高。

薄层色谱扫描法测定家兔血尿中艾司唑仑含量的研究

应用薄层色谱扫描法建立了家兔血尿中艾司唑仑的测定方法，具有快速、简便，准确的特点。通过对家兔血尿中艾司唑仑测定研究表明，服药２小时血浓度达峰时，尿中已能检出艾司唑仑。通过对数例艾司唑仑中毒病例血浓度的分析，初步确定艾司唑仑中毒血浓度在０．２０ｍｇ／１００ｍｌ以上。

HPLC测定体液内帕尼培南的浓度

目的 建立高效液相色谱法测定人血浆样品及尿样品中帕尼培南浓度的定量分析方法 ,探讨帕尼培南在人体内药物动力学过程。方法 以 1mol/L 3 (N 吗啉 )丙烷磺酸 ( pH7 0 )为稳定剂 ,对乙酰氨基酚为内标 ,在Diamosil(TM )C18柱 ( 5 μm ,2 5 0mm× 4 6mm )上 ,血样测定以甲醇∶0 0 4mol/L乙酸铵缓冲液( pH4 0 ,10∶90 )为流动相 ,流速 1 6ml/min ;尿样测定以甲醇∶0 0 4mol/L乙酸铵缓冲液 ( pH4 0 ,2∶98,)为流动相 ,流速 1 6ml/min ,检测波长 2 99 3nm ,对帕尼培南进行定量测定。结果 血浆中帕尼培南在 0 5～ 5 0 0 μg/ml浓度范围内具良好线性关系 (r =0 9999)。血浆中三种浓度 2 0、10 0、5 0 0 μg/ml平均回收率为 98 69%(n =6) ;三种浓度日内及日间RSD分别为 1 92 %、2 44 %、2 3 1% (n =5 )及 2 15 %、1 45 %、2 68% (n =3 )。尿中帕尼培南在 2～ 2 0 0 μg/ml浓度范围内具良好线性关系 (r =0 9999) ,尿中三种浓度 10 0、2 5 0、10 0 0 μg/ml平均回收率为 10 1 3 0 % (n =6) ;日内、日间RSD分别为 2 63 %、1 0 5 %、0 88% (n =5 )及 2 3 0 %、3 85 %、2 3 8% (n =3 )。结论 本方法简便、快捷、灵敏、准确 ,适用于临床药动学及药效学的研究。

豆腐虽好 不宜多吃

豆腐是人们公认的保健佳品。但是,豆腐并非多吃有益,过量也会危害健康。 因为制作豆腐的大豆,含有一种叫皂角苷的物质,它能预防动脉粥样硬化,但又能促进人体内碘的排泄。长期过量吃豆腐很容易引起碘的缺乏,发生碘缺乏病。

5-Fluorouracil concentration in blood, liver and tumor tissues and apoptosis of tumor cells after preoperative oral 5'-deoxy-5-fluorouridine in patients with hepatocellular carcinoma

AIM: To study the levels of 5-fluorouracail (5-FU) in plasma, liver and tumor in patients with hepatoceilular carcinoma after oral administration of 5'-deoxy-5-fluorouridine (5'-DFUR). METHODS: Thirty-nine patients with hepatoceilular carcinoma were treated with oral 5'-DFUR for more than 4 d before operation. The contents of 5-FU in plasma, liver and tumor were measured by high performance liquid chromatography (HPLC) and apoptosis of tumor cells was evaluated by in-situ TUNEL after resection of tumor. RESULTS: The concentrations of 5-FU were 1.1 μg/mL, 5.6, 5.9, and 10.5 μg/g in plasma, the liver tissue, the center of tumor and the periphery of tumor, respectively. 5-FU concentration was significantly higher in the periphery of tumor than that in the liver tissue and the center of tumor (10.5±1.6 μg/g vs 5.6±0.8 μg/g, t = 21.38, P<0.05; 10.5±1.6 μg/g vs 5.9±0.9 μg/g, t = 20.07, P<0.05). 5-FU level was significantly lower in plasma than that in the liver and the tumor (1.1±0.3 μg/mL vs 5.6±0.8 μg/g, t = 19.63, P<0.05; 1.1±0.3 μg/mL vs 10.5±1.6 μg/g, t= 41.01, P<0.05). Apoptosis of tumor cells was significantly increased after oral 5'-DFUR compared to the control group without 5-DFUR treatment. CONCLUSION: There is a higher concentration of 5-FU distributed in the tumor compared with liver tissue and apoptosis of tumor cells is increased following oral 5'-DFUR compared with the control group. The results indicate that 5'-DFUR is hopeful as neo-adjuvant chemotherapy to prevent recurrence after resection of hepatoceilular carcinoma.

The Relationships Between Milk Constituents and Various Milk Properties in Anatolian Buffaloes

The objectives of this study were to investigate the relationships among milk composition, renneting time, urea concentration, acidity, density and pH of Anatolian Buffaloes＇ milk. As a total of 115 individual milk samples from 53 Anatolian buffalo cows that calved in 2004 and 2005 on days of their lactations 30 ± 15, 60 ± 15, 90 ±15, 120 ± 15, 150 ± 15, 180± 15, 210 ±15, 240 ± 15 and 270 ± 15 in 8 units of lhkpmar village were collected in morning milkings in June, September, December and March. Samples were analysed for total dry matter （TDM）, fat, protein, ash, density, pH, acidity, renneting time and urea content. Data were classified according to the following environmental factors： lactation stages： （30 ± 15, 60 i 15, 90 ±15 days）： 2 （120±15, 150 ± 15, 180 ± 15 days）： 3 （210 ± 15, 240 ± 15, 270± 15 days）; calving year： 1 （2004）, 2 （2005）; calving season： 1 （January-May）, 2 （September and October）; month of samples collection： 1 （June）, 2 （September）, 3 （December）, 4 （March）; lactation order： 1 and 2：1, 3 and 4：2, 5 and 6：3. Means and correlation coefficients for the characteristics investigated were calculated. There were negative significant correlations between daily milk yield with TDM, fat and protein percentages, and between pH and all of the milk constituents. Density reduces as TDM, fat and protein contents increase. Relationships between density and coagulation time with milk yield and pH were not significant. Relationships between milk urea concentration with none of milk constituents, milk yield, density, pH and titratable acidity were not significant statistically. It was concluded that genetic selection has to be directed towards increasing fat, protein and total not fat dry matter yields. Under selection programs in which milk yield is taken into consideration, fat and protein yields also increase, but fat and protein concentrations decrease.

肾脏彩色多普勒超声对重症患者发生急性肾损伤的预测价值评估

综合评估肾脏彩色多普勒超声对重症患者发生急性肾损伤的预测价值。方法 本次研究的主要对象为:重症患者(共100例,病例选取时间开始于2021年3月,截止时间为2022年6月)。均留取重症患者的尿液标本和血液标本,检测患者的血肌酐浓度(采用苦味酸法)、血/尿胱抑素C浓度(采用乳胶增强免疫透射比浊法),与此同时开展彩色多普勒超声检查(获得重症患者的心输出量、肺动脉收缩压等)。采用统计学分析肾脏彩色多普勒超声、新型肾损伤标记物对重症患者是否急性肾损伤的预测价值以及发生急性肾损伤后的临床有关表现。结果 100例重症患者中有35例发生急性肾损伤(作为急性肾损伤组),占35.00%(35/100,22例为短暂性急性肾损伤、13例为持续性急性肾损伤);65例未发生急性肾损伤(作为非急性肾损伤组),占65.00%(65/100)。急性肾损伤组肾动脉阻力指数、肺动脉收缩压、血/尿胱抑素C浓度高于非急性肾损伤组(P<0.05),急性肾损伤组和非急性肾损伤组的平均动脉压比较无统计学意义(P>0.05),急性肾损伤组去甲肾上腺素用量大于非急性肾损伤组(P<0.05);短暂性急性肾损伤患者的体温、血胱抑素C浓度高于持续性急性肾损伤患者(P<0.05),短暂性急性肾损伤患者接受连续肾脏替代治疗的概率低于持续性急性肾损伤患者(P<0.05);二十四小时内发生急性肾损伤的预测价值评估:血/尿胱抑素C、肾动脉阻力指数的曲线下面积分别为0.754、0.774、0.668。结论 急性肾损伤患者的血/尿胱抑素C浓度、肾动脉阻力指数值比较高,持续性急性肾损伤患者的血胱抑素C浓度更高,血/尿胱抑素C浓度、肾动脉阻力指数可作为重症患者二十四小时内发生急性肾损伤的预测指标。