[Objective] In this study, the performance and blood biochemical indices in lamps fed on milk replacer were researched, the feasibility of early weaned in lamps was discussed. [Method] 37 of 10 days lambs of Suffolk...[Objective] In this study, the performance and blood biochemical indices in lamps fed on milk replacer were researched, the feasibility of early weaned in lamps was discussed. [Method] 37 of 10 days lambs of Suffolk(♂) x XiaoWeiHanYang( ♀ ) were randomly divided into group A, B, C, D for this experiment. Group A, B, C were fed by 3 milk-replacers containing milk for different protein source respectively, and ten Iambs in each group; group D (seven lambs) were fed by ewes as the control. The performance and blood biochemical indices of the lamps were determined. [ Result] The results showed that the average daily gains of 10 - 15 days of age was not significantly different between group A and D ( P 〉 O. 05 ), the control were higher significandy than the group B,C (P 〈0.05). The average daily gains of 16 -25, 26 -35 days of age were not significantly different in group A, B, C, D. The daily weight gains of Group A, B, C in 45 - 55 days of age were higher significantly than the control (P〈0.05 ). Plasma urea nitrogen (PUN) of group B and C were higher than group A significantly (P〈0.05 ) ;Total protein ( TP), Plasma albumin (ALB) and Plasma total amino acid were no significant difference among the other 3 groups (P〉0.05 ). [Conclusion] So, the milk-replacers were available for early-weaned lamb.展开更多
The effect of inhaled nano-TiO2 on lung histology and serum biochemical indexes is evaluated in healthy and adult Kunming mice(eight in each group)after exposure to TiO2 aerosols(1 500 mg/m3)in a sealed chamber.An...The effect of inhaled nano-TiO2 on lung histology and serum biochemical indexes is evaluated in healthy and adult Kunming mice(eight in each group)after exposure to TiO2 aerosols(1 500 mg/m3)in a sealed chamber.Another eight mice are exposed to indoor air to be served as a control group.Lung tissue and blood are collected after euthanizing the animals.The results show that lactate dehydrogenase(LDH)activity increases in all experimental groups.Alanine aminotransferase(ALT)activity and blood urea nitrogen(BUN)levels are increased in the group exposed to TiO2 aerosols for 28 d,and creatinine(Cr)levels are increased in 14 d and 28 d samples(P0.05).No obvious changes are observed in other serum indexes.Lungs of mice exposed to 28 d exposure show significant but moderate increase in pulmonary inflammation,and many TiO2 particles are found in the interstitium of pulmonary alveoli.展开更多
[Objective] This study aimed to investigate the effects of different padding on ammonia concentration in broiler house and growth performance and blood indi- cators of broilers. [Method] A total of 480 one-day-old 817...[Objective] This study aimed to investigate the effects of different padding on ammonia concentration in broiler house and growth performance and blood indi- cators of broilers. [Method] A total of 480 one-day-old 817 broilers were selected. They were randomly divided into four groups with different paddings, and each group was in triplicate. The weights of broilers and feed were weighed weekly. The growth performance of broilers was determined. On day 30, the ammonia concentra- tions in broiler houses were determined. On day 35, 6 broilers were randomly se- lected from each group, and their vein blood was sampled. In the sampled vein blood, the concentrations of ammonia, glucose, globulin, serum total protein and urea nitrogen were determined. [Result] There were significant differences in ammo- nia concentration among broiler houses laid with different paddings (P〈0.05). The ammonia concentration in group 1 was significantly lower than those in group 3 and group 4 (P〈0.05). No significant difference was found in ammonia concentrations in broiler houses between group 2 and group 3 (P〉0.05). The ammonia concentration in group 4 was significantly higher than those in group1, group 2 and group 3 (,0〈 0.05). There were also significant differences in blood indicators among different groups (P〈0.05). The blood ammonia, blood glucose and serum urea nitrogen con- centrations in group 1 were significantly lower than those in other groups (P〈0.05), the serum total protein concentration was significantly higher than those in group 3 and group 4 (P〈0.05), and the serum globulin concentration was significantly higher than those in group 2, group 3 and group 4 (P〈0.05). Significant differences were observed in feed intake, body weight gain and slaughter weight between different groups (P〈0.05), but there were no significant differences in feed to grain ratio (P〉 0.05). [Conclusion] Under the experimental conditions, the padding of Chinese medicine residue showed better effect on reducing ammonia concentration in broiler house, which was conducive to play of production potential of broilers.展开更多
[ Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff vari...[ Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff varieties. [ Method] A total of 103 blood samples were taken from four populations of Hequ Tibetan Mastiff, Qinhai Tibetan Mastiff, Tibetan Spaniel and native dogs of Qinghai. Seven blood protein Iocus(Tf, Po, Sα2, Hb, AIb, Pr and Amy)were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. Then the genetic variation during different populations was analyzed. [ Result] Genetic variations were observed in Tf, Sα2 and Po in four populations, others were not polymorphic. There were three alleles at the locus of Tf and Po, two alleles at the loci of Sα2. Effective number of alleles and Nei's average expected heterozygosity were 1. 532 4 and 0.230 3 relatively, all higher in Tibetan Mastiff than other populations. [ Conclusion] Protein locus in blood of Tibetan Mastiff existed in genetic variation.展开更多
[Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. [Method] From 27 pairs of porcine microsatellite primers recommended by Food and...[Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. [Method] From 27 pairs of porcine microsatellite primers recommended by Food and Agriculture Organization of the United Nations (FAO) and international Society for Animal Genetics (ISAG), six pairs (S0155, SW240, IGF1, SW951, SW857, SW24) were selected for microsatellite DNA detection of three Guizhou local pig breeds, including Nuogu Pig, Kele Pig and Guanling Pig. Subsequently, their genetic diversities were analyzed. [ Result] The three pig breeds were high polymorphic at the six microsatellite loci (PIC 〉0.5). The Nei's standard genetic distance of them was 0.206 3 -0.481 5. The genetic distance between Nuogu Pig and Kele Pig Was the closest, and that between Nuogu Pig and Guanling Pig was the furthest. [ Conclusion] The three Guizhou local pig breeds are in high genetic diversities. Nuogu Pig is a special type of Kele Pig, an excellent Chinese local pig breed.展开更多
The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, ...The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, was used to oral rats. At the end of study, their blood and thrombus were collected. The results show that TAICPM with the pore size of 1-5 μm can screen Dahuangzhechong Fang well. Dahuangzhechong Fang can increase 6-keto-PGF1α, lower content of TXD2 and platelet. Dahuangzhechong Fang has good effect to resist arterial thrombosis.展开更多
BNp/Al2O3-SiO2 system ceramic matrix composites with different volume fractions (10%-60%) of hexagonal BN particulates (BNp) were prepared by hot-press sintering technique. Phase components, microstructure, mechan...BNp/Al2O3-SiO2 system ceramic matrix composites with different volume fractions (10%-60%) of hexagonal BN particulates (BNp) were prepared by hot-press sintering technique. Phase components, microstructure, mechanical properties and plasma erosion resistance were also investigated. With the increase of h-BNp content, relative density and Vickers' hardness of the composite ceramics decrease, while the flexural strength, elastic modulus and fracture toughness increase and then decrease. The plasma erosion resistance linearly deteriorated with the increase of BNp content which is mainly determined by the density, crystal structure and atomic number of the elements.展开更多
Stem cell therapy holds great promises in medical treatment by, e.g., replacing lost cells, re-constitute healthy cell populations and also in the use of stem cells as vehicles for factor and gene delivery. Embryonic ...Stem cell therapy holds great promises in medical treatment by, e.g., replacing lost cells, re-constitute healthy cell populations and also in the use of stem cells as vehicles for factor and gene delivery. Embryonic stem cells have rightfully attracted a large interest due to their proven capacity of differentiating into any cell type in the embryo in vivo. Tissue-specific stem ceils are however already in use in medical practice, and recently the first systematic medical trials involving human neural stem cell (NSC) therapy have been launched. There are yet many obstacles to overcome and procedures to improve. To ensure progress in the medical use of stem cells increased basic knowledge of the molecular mechanisms that govern stem cell characteristics is necessary. Here we provide a review of the literature on NSCs in various aspects of cell therapy, with the main focus on the potential of using biomaterials to control NSC characteristics, differentiation, and delivery. We summarize results from studies on the characteristics of endogenous and transplanted NSCs in rodent models of neurological and cancer diseases, and highlight recent advancements in polymer compatibility and applicability in regulating NSC state and fate. We suggest that the development of specially designed polymers, such as hydrogels, is a crucial issue to improve the outcome of stem cell therapy in the central nervous system.展开更多
Cardiovascular disease is a leading cause of death throughout the world. The demand for new thera- peutic interventions is increasing. Although pharmacological and surgical interventions dramatically improve the quali...Cardiovascular disease is a leading cause of death throughout the world. The demand for new thera- peutic interventions is increasing. Although pharmacological and surgical interventions dramatically improve the quality of life of cardiovascular disease patients, cheaper and less invasive approaches are always preferable. Biomaterials, both natural and synthetic, exhibit great potential in cardiac repair and regeneration, either as a carrier for drug delivery or as an extracellular matrix substitute scaffold. In this review, we discuss the current treatment options for several cardiovascular diseases, as well as types of biomaterials that have been investigated as potential therapeutic interventions for said diseases. We especially highlight investigations into the possible use of conductive polymers for correcting ischemic heart disease-induced conduction abnormalities, and the generation of biological pacemakers to im- orove the conduction oathwav in heart block.展开更多
Here, we report our experience with a case of severe biliary bleeding due to a hepatic arterial pseudoaneurysm that had developed 1 year after endoscopic biliary plastic stent insertion. The patient, a 78-year-old wom...Here, we report our experience with a case of severe biliary bleeding due to a hepatic arterial pseudoaneurysm that had developed 1 year after endoscopic biliary plastic stent insertion. The patient, a 78-year-old woman, presented with hematemesis and obstructive jaundice. Ruptured hepatic arterial pseudoaneurysm was diagnosed, which was suspected to have been caused by long-term placement of an endoscopic retrograde biliary drainage(ERBD) stent. This episode of biliary bleeding was successfully treated by transarterial embolization(TAE). Pseudoaneurysm leading to hemobilia is a rare but potentially fatal complication in patients with long-term placement of ERBD. TAE is a minimally invasive procedure that offers effective treatment for biliary bleeding.展开更多
In the current studies a miniature silicon wafer fuel cell(FC) using L-ascorbic acid as fuel was developed. The cell employs L-ascorbic acid and air as reactants and a thin polymer electrolyte as a separator. Inductiv...In the current studies a miniature silicon wafer fuel cell(FC) using L-ascorbic acid as fuel was developed. The cell employs L-ascorbic acid and air as reactants and a thin polymer electrolyte as a separator. Inductively coupled plasma(ICP) silicon etching was employed to fabricate high aspect-ratio columns on the silicon substrate to increase the surface area. A thin platinum layer deposited directly on the silicon surface by the sputtering was used as the catalyst layer for L-ascorbic acid electro-oxidation. Cyclic voltammetry shows that the oxidation of L-ascorbic acid on the sputtered platinum layer is irreversible and that the onset potentials for the oxidation of L-ascorbic acid are from 0.27 V to 0.35 V versus an Ag/AgCl reference electrode. It is found that at the room temperature,with 1 mol/L L-ascorbic acid/PBS(phosphate buffered solution) solution pumped to the anode at 1 ml/min flow rate and air spontaneously diffusing to the cathode as the oxidant,the maximum output power density of the cell was 1.95 mW/cm2 at a current density of 10 mA/cm2.展开更多
Objective Global cardiovascular risk assessment has been incorporated into current Chinese guidelines for the management of hypertension and hypercholesterolemia. The aim of our study is to determine the distribution ...Objective Global cardiovascular risk assessment has been incorporated into current Chinese guidelines for the management of hypertension and hypercholesterolemia. The aim of our study is to determine the distribution of 10-year risk for ischemic cardiovascular disease (ICVD) among middle-aged Chinese adults, and to evaluate the usefulness of global risk assessment tools in the primary prevention of ICVD in Chinese population.Methods Simplified prediction tools derived from the USA-PRC Collaborative Study of Cardiovascular Epidemiology cohort were applied to the Chinese Health Examination Database (CHED) 2008. 10-year risk for ICVD was estimated in 461 157 ICVD-free subjects (264 432 male and 196 725 female) aged 35 to 59 years. Results Among the male subjects, 82.4% had a 10-year risk for ICVD of 〈5%, 14.4% of 5% to 12.1% and 3.4 of≥ 12.2%, and in female subjects, 86.7% had a 10-year risk for ICVD of 〈5%, 12.1% of 5% to 12.0% and 1.2% of ≥ 12.1%. All subjects with predicted high level ICVD risk (≥ 12.2% in male or ≥ 12.1% in female) had either remarkably elevated (≥ 160 mmHg) blood pressure, significantly increased (≥ 6.22 mml/L) total serum cholesterol or diabetes.Conclusion Using the currently recommended prediction tools, only very small proportions of middle-aged Chinese men and women who were free of ICVD would be classified into high level risk group. These prediction tools are unlikely to help for the medical intervention decision making in Chinese adult patients with hypertension and/or hypercholesterolemia展开更多
基金Supported by Span Program of Ministry of Agriculture(KY2001-15)~~
文摘[Objective] In this study, the performance and blood biochemical indices in lamps fed on milk replacer were researched, the feasibility of early weaned in lamps was discussed. [Method] 37 of 10 days lambs of Suffolk(♂) x XiaoWeiHanYang( ♀ ) were randomly divided into group A, B, C, D for this experiment. Group A, B, C were fed by 3 milk-replacers containing milk for different protein source respectively, and ten Iambs in each group; group D (seven lambs) were fed by ewes as the control. The performance and blood biochemical indices of the lamps were determined. [ Result] The results showed that the average daily gains of 10 - 15 days of age was not significantly different between group A and D ( P 〉 O. 05 ), the control were higher significandy than the group B,C (P 〈0.05). The average daily gains of 16 -25, 26 -35 days of age were not significantly different in group A, B, C, D. The daily weight gains of Group A, B, C in 45 - 55 days of age were higher significantly than the control (P〈0.05 ). Plasma urea nitrogen (PUN) of group B and C were higher than group A significantly (P〈0.05 ) ;Total protein ( TP), Plasma albumin (ALB) and Plasma total amino acid were no significant difference among the other 3 groups (P〉0.05 ). [Conclusion] So, the milk-replacers were available for early-weaned lamb.
文摘The effect of inhaled nano-TiO2 on lung histology and serum biochemical indexes is evaluated in healthy and adult Kunming mice(eight in each group)after exposure to TiO2 aerosols(1 500 mg/m3)in a sealed chamber.Another eight mice are exposed to indoor air to be served as a control group.Lung tissue and blood are collected after euthanizing the animals.The results show that lactate dehydrogenase(LDH)activity increases in all experimental groups.Alanine aminotransferase(ALT)activity and blood urea nitrogen(BUN)levels are increased in the group exposed to TiO2 aerosols for 28 d,and creatinine(Cr)levels are increased in 14 d and 28 d samples(P0.05).No obvious changes are observed in other serum indexes.Lungs of mice exposed to 28 d exposure show significant but moderate increase in pulmonary inflammation,and many TiO2 particles are found in the interstitium of pulmonary alveoli.
基金Supported by Innovation Project for Universities and Research Institutes in Jinan City(201303053)Shandong Provincial Science and Technology Plan Project(2014GGH210001)Program of Animal Husbandry and Veterinary Bureau of Shandong Province~~
文摘[Objective] This study aimed to investigate the effects of different padding on ammonia concentration in broiler house and growth performance and blood indi- cators of broilers. [Method] A total of 480 one-day-old 817 broilers were selected. They were randomly divided into four groups with different paddings, and each group was in triplicate. The weights of broilers and feed were weighed weekly. The growth performance of broilers was determined. On day 30, the ammonia concentra- tions in broiler houses were determined. On day 35, 6 broilers were randomly se- lected from each group, and their vein blood was sampled. In the sampled vein blood, the concentrations of ammonia, glucose, globulin, serum total protein and urea nitrogen were determined. [Result] There were significant differences in ammo- nia concentration among broiler houses laid with different paddings (P〈0.05). The ammonia concentration in group 1 was significantly lower than those in group 3 and group 4 (P〈0.05). No significant difference was found in ammonia concentrations in broiler houses between group 2 and group 3 (P〉0.05). The ammonia concentration in group 4 was significantly higher than those in group1, group 2 and group 3 (,0〈 0.05). There were also significant differences in blood indicators among different groups (P〈0.05). The blood ammonia, blood glucose and serum urea nitrogen con- centrations in group 1 were significantly lower than those in other groups (P〈0.05), the serum total protein concentration was significantly higher than those in group 3 and group 4 (P〈0.05), and the serum globulin concentration was significantly higher than those in group 2, group 3 and group 4 (P〈0.05). Significant differences were observed in feed intake, body weight gain and slaughter weight between different groups (P〈0.05), but there were no significant differences in feed to grain ratio (P〉 0.05). [Conclusion] Under the experimental conditions, the padding of Chinese medicine residue showed better effect on reducing ammonia concentration in broiler house, which was conducive to play of production potential of broilers.
基金Supported by Foundation of Gansu Technology Committee (GKC-97-27-5)Youth Foundation of Tianshui Normal University (X4-25)~~
文摘[ Objective] The aim was to study the protein polymorphism in the blood of Tibetan Mastiff, and provide some theoretical basis for resource protection and reasonable development and utilization of Tibetan Mastiff varieties. [ Method] A total of 103 blood samples were taken from four populations of Hequ Tibetan Mastiff, Qinhai Tibetan Mastiff, Tibetan Spaniel and native dogs of Qinghai. Seven blood protein Iocus(Tf, Po, Sα2, Hb, AIb, Pr and Amy)were investigated by using vertical polyacrylamide gel electrophoresis with discontinuous buffer system. Then the genetic variation during different populations was analyzed. [ Result] Genetic variations were observed in Tf, Sα2 and Po in four populations, others were not polymorphic. There were three alleles at the locus of Tf and Po, two alleles at the loci of Sα2. Effective number of alleles and Nei's average expected heterozygosity were 1. 532 4 and 0.230 3 relatively, all higher in Tibetan Mastiff than other populations. [ Conclusion] Protein locus in blood of Tibetan Mastiff existed in genetic variation.
基金Supported by Key Project of Agriculture in Guizhou Province (NY[2008]3042)~~
文摘[Objective] The research aimed to provide the genetic basis for the protection, development and utilization of Guizhou local pig breeds. [Method] From 27 pairs of porcine microsatellite primers recommended by Food and Agriculture Organization of the United Nations (FAO) and international Society for Animal Genetics (ISAG), six pairs (S0155, SW240, IGF1, SW951, SW857, SW24) were selected for microsatellite DNA detection of three Guizhou local pig breeds, including Nuogu Pig, Kele Pig and Guanling Pig. Subsequently, their genetic diversities were analyzed. [ Result] The three pig breeds were high polymorphic at the six microsatellite loci (PIC 〉0.5). The Nei's standard genetic distance of them was 0.206 3 -0.481 5. The genetic distance between Nuogu Pig and Kele Pig Was the closest, and that between Nuogu Pig and Guanling Pig was the furthest. [ Conclusion] The three Guizhou local pig breeds are in high genetic diversities. Nuogu Pig is a special type of Kele Pig, an excellent Chinese local pig breed.
基金Project (2010FA32370) supported by The Ministry of Science and Technology of ChinaProject (2008WK3002) supported by Hunan Provincial Science and Technology DepartmentProject (20060390891) supported by the Postdoctoral Science Foundation of China
文摘The mechanism of antithrombotic of Dahuangzhechong Fang separated and purified by Ti-Al intermetallic compound porous material (TAICPM) was researched. Dahuangzhechong Fang, which was isolated and screened by TAICPM, was used to oral rats. At the end of study, their blood and thrombus were collected. The results show that TAICPM with the pore size of 1-5 μm can screen Dahuangzhechong Fang well. Dahuangzhechong Fang can increase 6-keto-PGF1α, lower content of TXD2 and platelet. Dahuangzhechong Fang has good effect to resist arterial thrombosis.
基金Project(HIT.NSRIF.2010112)supported by the Fundamental Research Fund for the Central Universities,ChinaProjects(50902030,51021002)supported by the National Natural Science Foundation of China
文摘BNp/Al2O3-SiO2 system ceramic matrix composites with different volume fractions (10%-60%) of hexagonal BN particulates (BNp) were prepared by hot-press sintering technique. Phase components, microstructure, mechanical properties and plasma erosion resistance were also investigated. With the increase of h-BNp content, relative density and Vickers' hardness of the composite ceramics decrease, while the flexural strength, elastic modulus and fracture toughness increase and then decrease. The plasma erosion resistance linearly deteriorated with the increase of BNp content which is mainly determined by the density, crystal structure and atomic number of the elements.
文摘Stem cell therapy holds great promises in medical treatment by, e.g., replacing lost cells, re-constitute healthy cell populations and also in the use of stem cells as vehicles for factor and gene delivery. Embryonic stem cells have rightfully attracted a large interest due to their proven capacity of differentiating into any cell type in the embryo in vivo. Tissue-specific stem ceils are however already in use in medical practice, and recently the first systematic medical trials involving human neural stem cell (NSC) therapy have been launched. There are yet many obstacles to overcome and procedures to improve. To ensure progress in the medical use of stem cells increased basic knowledge of the molecular mechanisms that govern stem cell characteristics is necessary. Here we provide a review of the literature on NSCs in various aspects of cell therapy, with the main focus on the potential of using biomaterials to control NSC characteristics, differentiation, and delivery. We summarize results from studies on the characteristics of endogenous and transplanted NSCs in rodent models of neurological and cancer diseases, and highlight recent advancements in polymer compatibility and applicability in regulating NSC state and fate. We suggest that the development of specially designed polymers, such as hydrogels, is a crucial issue to improve the outcome of stem cell therapy in the central nervous system.
文摘Cardiovascular disease is a leading cause of death throughout the world. The demand for new thera- peutic interventions is increasing. Although pharmacological and surgical interventions dramatically improve the quality of life of cardiovascular disease patients, cheaper and less invasive approaches are always preferable. Biomaterials, both natural and synthetic, exhibit great potential in cardiac repair and regeneration, either as a carrier for drug delivery or as an extracellular matrix substitute scaffold. In this review, we discuss the current treatment options for several cardiovascular diseases, as well as types of biomaterials that have been investigated as potential therapeutic interventions for said diseases. We especially highlight investigations into the possible use of conductive polymers for correcting ischemic heart disease-induced conduction abnormalities, and the generation of biological pacemakers to im- orove the conduction oathwav in heart block.
基金Supported by Japanese Red Cross Kyoto Daiichi Hospital,Kyoto,Japan
文摘Here, we report our experience with a case of severe biliary bleeding due to a hepatic arterial pseudoaneurysm that had developed 1 year after endoscopic biliary plastic stent insertion. The patient, a 78-year-old woman, presented with hematemesis and obstructive jaundice. Ruptured hepatic arterial pseudoaneurysm was diagnosed, which was suspected to have been caused by long-term placement of an endoscopic retrograde biliary drainage(ERBD) stent. This episode of biliary bleeding was successfully treated by transarterial embolization(TAE). Pseudoaneurysm leading to hemobilia is a rare but potentially fatal complication in patients with long-term placement of ERBD. TAE is a minimally invasive procedure that offers effective treatment for biliary bleeding.
基金the National Natural Science Foundation of China (No. 30670535)the Program for New Century Excellent Talents in University (No. NCET-07-0752), China
文摘In the current studies a miniature silicon wafer fuel cell(FC) using L-ascorbic acid as fuel was developed. The cell employs L-ascorbic acid and air as reactants and a thin polymer electrolyte as a separator. Inductively coupled plasma(ICP) silicon etching was employed to fabricate high aspect-ratio columns on the silicon substrate to increase the surface area. A thin platinum layer deposited directly on the silicon surface by the sputtering was used as the catalyst layer for L-ascorbic acid electro-oxidation. Cyclic voltammetry shows that the oxidation of L-ascorbic acid on the sputtered platinum layer is irreversible and that the onset potentials for the oxidation of L-ascorbic acid are from 0.27 V to 0.35 V versus an Ag/AgCl reference electrode. It is found that at the room temperature,with 1 mol/L L-ascorbic acid/PBS(phosphate buffered solution) solution pumped to the anode at 1 ml/min flow rate and air spontaneously diffusing to the cathode as the oxidant,the maximum output power density of the cell was 1.95 mW/cm2 at a current density of 10 mA/cm2.
文摘Objective Global cardiovascular risk assessment has been incorporated into current Chinese guidelines for the management of hypertension and hypercholesterolemia. The aim of our study is to determine the distribution of 10-year risk for ischemic cardiovascular disease (ICVD) among middle-aged Chinese adults, and to evaluate the usefulness of global risk assessment tools in the primary prevention of ICVD in Chinese population.Methods Simplified prediction tools derived from the USA-PRC Collaborative Study of Cardiovascular Epidemiology cohort were applied to the Chinese Health Examination Database (CHED) 2008. 10-year risk for ICVD was estimated in 461 157 ICVD-free subjects (264 432 male and 196 725 female) aged 35 to 59 years. Results Among the male subjects, 82.4% had a 10-year risk for ICVD of 〈5%, 14.4% of 5% to 12.1% and 3.4 of≥ 12.2%, and in female subjects, 86.7% had a 10-year risk for ICVD of 〈5%, 12.1% of 5% to 12.0% and 1.2% of ≥ 12.1%. All subjects with predicted high level ICVD risk (≥ 12.2% in male or ≥ 12.1% in female) had either remarkably elevated (≥ 160 mmHg) blood pressure, significantly increased (≥ 6.22 mml/L) total serum cholesterol or diabetes.Conclusion Using the currently recommended prediction tools, only very small proportions of middle-aged Chinese men and women who were free of ICVD would be classified into high level risk group. These prediction tools are unlikely to help for the medical intervention decision making in Chinese adult patients with hypertension and/or hypercholesterolemia