Effects of berberine (Ber) on platelet aggregation and TXB2 and 6 keto PGF1a plasma levels were studied in rabbits with uncomplete cerebral ischemia. Ber inhibited uncomplete cerebral ischemic rabbit platelet aggreg...Effects of berberine (Ber) on platelet aggregation and TXB2 and 6 keto PGF1a plasma levels were studied in rabbits with uncomplete cerebral ischemia. Ber inhibited uncomplete cerebral ischemic rabbit platelet aggregation triggered by collagen, ADP, and arachidonic acid (AA) with the IC 50 of 0.15, 0.46, and 0.51 mg·ml 1 , respectively. In rabbits, Ber 25, or 50 mg·kg 1 iv 30 min after uncomplete cerebral ischemia, restrained the collagen ADP and AA induced platelet aggregation determined 90 min later. With radioimmunoassay, we measured the thromboxane B2 (TXB 2) and 6 ketoprostaglandin F 1α (6 keto PGF 1α ) contents in rabbit plasma. The results indicated that the TXB 2 level in rabbit 120 min after uncomplete cerebral ischemia (921±539 pg·ml 1 ) was higher than that (230±71 pg·ml 1 ) in normal rabbits ( P < 0.01), but 6 keto PGF 1α level after ischemia (73±23pg·ml 1 ) was lower than that (262±988pg·ml 1 ) in normal rabbit. Ber (5, 25 or 50 mg·kg 1 ) reduced obviously the plasma TXB 2 level in rabbit with uncomplete cerebral ischemia (504±196, 386±174, or 272±183 vs 921±539 pg·ml 1 , respectively, P < 0.01). We conclude that the decrease of TXB 2 content is one of the possible mechanisms of Ber anti cerebral ischemic effect.展开更多
Objective:To observe the effects of acupuncture at different times on plasma thromboxane(TXB2) and prostaglandin 6-Keto-PGF1a(6-K-P) in patients with ischemic cerebrovascular disease.Methods:Totally 90 patients were r...Objective:To observe the effects of acupuncture at different times on plasma thromboxane(TXB2) and prostaglandin 6-Keto-PGF1a(6-K-P) in patients with ischemic cerebrovascular disease.Methods:Totally 90 patients were randomly divided into a group acupunctured at 7-9 am,a group acupunctured at 3-5 pm and a drug control group,with 30 cases in each group.The contents of plasma TXB2 and 6-K-P from venous blood before treatment were compared with those 15 days after treatment.Results:The plasma TXB2 levels of the two acupuncture groups were obviously lower than those before treatment(P<0.05,P<0.01) ,but the 6-K-P levels of both the acupuncture groups were remarkably higher than those before treatment(P<0.05,P<0.01) . And the TXB2 level in the 3-5 pm acupuncture group was obviously lower than that in the 7-9 am acupuncture group(P<0.05) ,and the 6-K-P level of the former was obviously higher than that of the latter(P<0.05) .Conclusion:Acupuncture can promote functional recovery in patients with ischemic cerebrovascular disease and enhance their survival quality.展开更多
文摘Effects of berberine (Ber) on platelet aggregation and TXB2 and 6 keto PGF1a plasma levels were studied in rabbits with uncomplete cerebral ischemia. Ber inhibited uncomplete cerebral ischemic rabbit platelet aggregation triggered by collagen, ADP, and arachidonic acid (AA) with the IC 50 of 0.15, 0.46, and 0.51 mg·ml 1 , respectively. In rabbits, Ber 25, or 50 mg·kg 1 iv 30 min after uncomplete cerebral ischemia, restrained the collagen ADP and AA induced platelet aggregation determined 90 min later. With radioimmunoassay, we measured the thromboxane B2 (TXB 2) and 6 ketoprostaglandin F 1α (6 keto PGF 1α ) contents in rabbit plasma. The results indicated that the TXB 2 level in rabbit 120 min after uncomplete cerebral ischemia (921±539 pg·ml 1 ) was higher than that (230±71 pg·ml 1 ) in normal rabbits ( P < 0.01), but 6 keto PGF 1α level after ischemia (73±23pg·ml 1 ) was lower than that (262±988pg·ml 1 ) in normal rabbit. Ber (5, 25 or 50 mg·kg 1 ) reduced obviously the plasma TXB 2 level in rabbit with uncomplete cerebral ischemia (504±196, 386±174, or 272±183 vs 921±539 pg·ml 1 , respectively, P < 0.01). We conclude that the decrease of TXB 2 content is one of the possible mechanisms of Ber anti cerebral ischemic effect.
文摘Objective:To observe the effects of acupuncture at different times on plasma thromboxane(TXB2) and prostaglandin 6-Keto-PGF1a(6-K-P) in patients with ischemic cerebrovascular disease.Methods:Totally 90 patients were randomly divided into a group acupunctured at 7-9 am,a group acupunctured at 3-5 pm and a drug control group,with 30 cases in each group.The contents of plasma TXB2 and 6-K-P from venous blood before treatment were compared with those 15 days after treatment.Results:The plasma TXB2 levels of the two acupuncture groups were obviously lower than those before treatment(P<0.05,P<0.01) ,but the 6-K-P levels of both the acupuncture groups were remarkably higher than those before treatment(P<0.05,P<0.01) . And the TXB2 level in the 3-5 pm acupuncture group was obviously lower than that in the 7-9 am acupuncture group(P<0.05) ,and the 6-K-P level of the former was obviously higher than that of the latter(P<0.05) .Conclusion:Acupuncture can promote functional recovery in patients with ischemic cerebrovascular disease and enhance their survival quality.
