据《The New England Journal of Medicine》1997年337卷第4期报道 已有研究表明血浆高半胱氨酸(homocysteine)水平的升高是冠心病的危险因素,但已被确诊为冠脉病病人的高半胱氨酸水平的预后值却尚未确定。Nygard等研究者对587例经血管...据《The New England Journal of Medicine》1997年337卷第4期报道 已有研究表明血浆高半胱氨酸(homocysteine)水平的升高是冠心病的危险因素,但已被确诊为冠脉病病人的高半胱氨酸水平的预后值却尚未确定。Nygard等研究者对587例经血管造影确诊的冠脉病人,其血浆总高半胱氨酸水平与死亡率之间的关系进行了前瞻性研究。展开更多
Objective. To investigate the relationship of plasma homocysteine (Hcy) level to stroke and genetic factor to elevated plasma Hcy level.Methods. The plasma Hcy level was measured by capillary electrophoresis- ultravio...Objective. To investigate the relationship of plasma homocysteine (Hcy) level to stroke and genetic factor to elevated plasma Hcy level.Methods. The plasma Hcy level was measured by capillary electrophoresis- ultraviolet detection and the gene polymorphism of N5, N10 - methylenetetrahydrofolate reductase (MTHFR) was studied with PCR - RFLP assay in 43 patients with cortical cerebral infarction and 42 healthy controls.Results. The plasma Hcy level of the patients ( 19. 3 + 6. 0 μ mol/L) was markedly higher than that of the controls (13.7 + 5.4 μ mol/L) ( t = 4. 16, P ＜ 0. 001). There are 3 genotypes, C/C, C/T and T/T, about base - variation of MTHFR gene at locus 677. The plasma Hcy level of the subjects with T/T genotype was higher than that of subjects with other genotypes. However, the frequencies of each genotype and allele were not significantly different between the patients and the controls.Conclusions. The elevated plasma Hcy level is a risk factor for atherothrombotic cerebral infarction, and is related to the C→T mutation at locus 677 of MTHFR gene.展开更多
文摘据《The New England Journal of Medicine》1997年337卷第4期报道 已有研究表明血浆高半胱氨酸(homocysteine)水平的升高是冠心病的危险因素,但已被确诊为冠脉病病人的高半胱氨酸水平的预后值却尚未确定。Nygard等研究者对587例经血管造影确诊的冠脉病人,其血浆总高半胱氨酸水平与死亡率之间的关系进行了前瞻性研究。
基金This study was supported by"333 Talent Project " funds of Jiangsu Province in 1999.
文摘Objective. To investigate the relationship of plasma homocysteine (Hcy) level to stroke and genetic factor to elevated plasma Hcy level.Methods. The plasma Hcy level was measured by capillary electrophoresis- ultraviolet detection and the gene polymorphism of N5, N10 - methylenetetrahydrofolate reductase (MTHFR) was studied with PCR - RFLP assay in 43 patients with cortical cerebral infarction and 42 healthy controls.Results. The plasma Hcy level of the patients ( 19. 3 + 6. 0 μ mol/L) was markedly higher than that of the controls (13.7 + 5.4 μ mol/L) ( t = 4. 16, P ＜ 0. 001). There are 3 genotypes, C/C, C/T and T/T, about base - variation of MTHFR gene at locus 677. The plasma Hcy level of the subjects with T/T genotype was higher than that of subjects with other genotypes. However, the frequencies of each genotype and allele were not significantly different between the patients and the controls.Conclusions. The elevated plasma Hcy level is a risk factor for atherothrombotic cerebral infarction, and is related to the C→T mutation at locus 677 of MTHFR gene.
