研究复方中药添加剂对仔猪生产性能和血液生理指数的影响。试验断奶仔猪随机分为4组,每组10头,分别为对照组、低剂量组、中剂量组和高剂量组,分别在日粮中添加0、0.5%、1.0%和2.0%复方中药添加剂,饲养21 d。在第21天饲喂前空腹称质量记...研究复方中药添加剂对仔猪生产性能和血液生理指数的影响。试验断奶仔猪随机分为4组,每组10头,分别为对照组、低剂量组、中剂量组和高剂量组,分别在日粮中添加0、0.5%、1.0%和2.0%复方中药添加剂,饲养21 d。在第21天饲喂前空腹称质量记录体质量,计算平均日采食量(ADFI)、平均日增质量(ADG)和料重比(F/G)。前腔静脉空腹采集全血5 m L。测定全血中红细胞计数(RBC)、红细胞压积(HCT)、血红蛋白(HGB)、血小板计数(PLT)和白细胞计数(WBC)。在断乳仔猪日粮中添加0.5%、1.0%和2.0%复方中药添加剂,仔猪的平均日增质量、平均日采食量有所提高和料重比有下降趋势,但各组间差异不显著(P>0.05)。低剂量组、中剂量组和高剂量组仔猪血液中白细胞数都显著高于对照组(P<0.05),血液血小板数显著低于对照组(P<0.05),红细胞压积各组间差异不显著(P>0.05)。中剂量组和高剂量组的仔猪血液中红细胞数和血红蛋白含量显著高于对照组(P<0.05)。展开更多
目的探究急性脑梗死患者施行丁苯肽联合脑循环治疗仪的临床疗效,并对神经功能和脑循环状态的影响。方法回顾2018年5月—2020年5月收治急性脑梗死共86例患者资料,结合疗法差异分成联合组45例和参照组41例。参照组施行急性脑梗死常规治疗...目的探究急性脑梗死患者施行丁苯肽联合脑循环治疗仪的临床疗效,并对神经功能和脑循环状态的影响。方法回顾2018年5月—2020年5月收治急性脑梗死共86例患者资料,结合疗法差异分成联合组45例和参照组41例。参照组施行急性脑梗死常规治疗。联合组在施行脑循环治疗仪基础上给予丁苯肽氯化钠注射液,对比两组于神经缺损得分(national institute of health stroke scale,NIHSS)、血基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)含量上的差异性,并利用多普勒超声评估治疗前后颈内动脉平均血流速度、脑血管阻力变化。结果联合组NIHSS量表评分及MMP-2表达水平明显低于参照组,比较差异有统计学意义(P<0.05);联合组治疗后颈内动脉平均血流速度比参照组有一定程度上升,脑血管阻力比参照组有一定降低(P<0.05)。结论脑循环治疗仪联合丁苯肽氯化钠注射液治疗在急性脑梗死治疗方面有显著疗效,改善患者神经功能缺损和改善脑循环状态。展开更多
Throughout the animal kingdom there are species that have two or more phenotypic forms or ‘morphs＇, and many of these are amphibians. In North America, the red-backed salamander Plethodon cinereus can have either a ...Throughout the animal kingdom there are species that have two or more phenotypic forms or ‘morphs＇, and many of these are amphibians. In North America, the red-backed salamander Plethodon cinereus can have either a red dorsal stripe or no dorsal stripe （lead-phase form）, and evidence to date indicates the lead-phase form incurs a greater number of attacks from predators. In a recent collection of 51 P cinereus, blood smears of both color morphs （35 red-stripe, 16 lead-phase） were examined to obtain numbers of circulating leukocytes （via light microscopy）, which can be used to indirectly estimate levels of stress hormones in vertebrates via a ‘hematological stress index＇, which is the ratio between the number of two leukocyte types （neutrophils and lymphocytes）. Our results showed that lead-phase salamanders tended to have greater numbers of circulating neutrophils and lower numbers of circulating lymphocytes than red-stripe morphs, leading to higher average neutrophil-lymphocyte ratios in lead-phase individuals. Since the salamanders were held （refrigerated） for 7 days before sampling, we cannot be certain if this effect is a stress reaction to the captivity or the normal level for this morph. However comparison with two sets of related salamanders that were captured and sampled immediately indicates the red-stripe salamanders were either not stressed from the captivity at all, or their white blood cell distributions had returned to normal after 7 days of captivity. Taken together, our results indicate that lead-phase forms of P. cinereus have higher stress levels than the red-stripe forms, which may be a consequence of their higher exposure to, and/or attacks from, predators. They may also indicate that the lead-phase form is less-suited to captivity than the red-stripe form of this species.展开更多
Heat stress affects feed intake, milk production, and endocrine status in dairy cows. The temperaturehumidity index（THI） is employed as an index to evaluate the degree of heat stress in dairy cows. However, it is di...Heat stress affects feed intake, milk production, and endocrine status in dairy cows. The temperaturehumidity index（THI） is employed as an index to evaluate the degree of heat stress in dairy cows. However, it is difficult to ascertain whether THI is the most appropriate measurement of heat stress in dairy cows. This experiment was conducted to investigate the effects of heat stress on serum insulin, adipokines（leptin and adiponectin）, AMP-activated protein kinase（AMPK）, and heat shock signal molecules（heat shock transcription factor（HSF） and heat shock proteins（HSP）） in dairy cows and to research biomarkers to be used for better understanding the meaning of THI as a bioclimatic index. To achieve these objectives, two experiments were performed. The first experiment： eighteen lactating Holstein dairy cows were used. The treatments were： heat stress（HS, THI average=81.7, n=9） and cooling（CL, THI average=53.4, n=9）. Samples of HS were obtained on August 16, 2013, and samples of CL were collected on April 7, 2014 in natural conditions. The second experiment： HS treatment cows（n=9） from the first experiment were fed for 8 weeks from August 16, 2013 to October 12, 2013. Samples for moderate heat stress, mild heat stress, and no heat stress were obtained, respectively, according to the physical alterations of the THI. Results showed that heat stress significantly increased the serum adiponectin, AMPK, HSF, HSP27, HSP70, and HSP90（P〈0.05）. Adiponectin is strongly associated with AMPK. The increases of adiponectin and AMPK may be one of the mechanisms to maintain homeostasis in heat-stressed dairy cows. When heat stress treatment lasted 8 weeks, a higher expression of HSF and HSP70 was observed under moderate heat stress. Serum HSF and HSP70 are sensitive and accurate in heat stress and they could be potential indicators of animal response to heat stress. We recommend serum HSF and HSP70 as meaningful biomarkers to supplement the THI and evaluate moderate heat stress in dairy cows in the future.展开更多
文摘研究复方中药添加剂对仔猪生产性能和血液生理指数的影响。试验断奶仔猪随机分为4组,每组10头,分别为对照组、低剂量组、中剂量组和高剂量组,分别在日粮中添加0、0.5%、1.0%和2.0%复方中药添加剂,饲养21 d。在第21天饲喂前空腹称质量记录体质量,计算平均日采食量(ADFI)、平均日增质量(ADG)和料重比(F/G)。前腔静脉空腹采集全血5 m L。测定全血中红细胞计数(RBC)、红细胞压积(HCT)、血红蛋白(HGB)、血小板计数(PLT)和白细胞计数(WBC)。在断乳仔猪日粮中添加0.5%、1.0%和2.0%复方中药添加剂,仔猪的平均日增质量、平均日采食量有所提高和料重比有下降趋势,但各组间差异不显著(P>0.05)。低剂量组、中剂量组和高剂量组仔猪血液中白细胞数都显著高于对照组(P<0.05),血液血小板数显著低于对照组(P<0.05),红细胞压积各组间差异不显著(P>0.05)。中剂量组和高剂量组的仔猪血液中红细胞数和血红蛋白含量显著高于对照组(P<0.05)。
文摘目的探究急性脑梗死患者施行丁苯肽联合脑循环治疗仪的临床疗效,并对神经功能和脑循环状态的影响。方法回顾2018年5月—2020年5月收治急性脑梗死共86例患者资料,结合疗法差异分成联合组45例和参照组41例。参照组施行急性脑梗死常规治疗。联合组在施行脑循环治疗仪基础上给予丁苯肽氯化钠注射液,对比两组于神经缺损得分(national institute of health stroke scale,NIHSS)、血基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)含量上的差异性,并利用多普勒超声评估治疗前后颈内动脉平均血流速度、脑血管阻力变化。结果联合组NIHSS量表评分及MMP-2表达水平明显低于参照组,比较差异有统计学意义(P<0.05);联合组治疗后颈内动脉平均血流速度比参照组有一定程度上升,脑血管阻力比参照组有一定降低(P<0.05)。结论脑循环治疗仪联合丁苯肽氯化钠注射液治疗在急性脑梗死治疗方面有显著疗效,改善患者神经功能缺损和改善脑循环状态。
基金Funding for AKD during this project was provided by the DBWarnell School of Forestry and Natural Resources and a grant from the Morris Animal FoundationSupport for the field word of this project came form an NSF grant (NSF-DEB DEB0542974)
文摘Throughout the animal kingdom there are species that have two or more phenotypic forms or ‘morphs＇, and many of these are amphibians. In North America, the red-backed salamander Plethodon cinereus can have either a red dorsal stripe or no dorsal stripe （lead-phase form）, and evidence to date indicates the lead-phase form incurs a greater number of attacks from predators. In a recent collection of 51 P cinereus, blood smears of both color morphs （35 red-stripe, 16 lead-phase） were examined to obtain numbers of circulating leukocytes （via light microscopy）, which can be used to indirectly estimate levels of stress hormones in vertebrates via a ‘hematological stress index＇, which is the ratio between the number of two leukocyte types （neutrophils and lymphocytes）. Our results showed that lead-phase salamanders tended to have greater numbers of circulating neutrophils and lower numbers of circulating lymphocytes than red-stripe morphs, leading to higher average neutrophil-lymphocyte ratios in lead-phase individuals. Since the salamanders were held （refrigerated） for 7 days before sampling, we cannot be certain if this effect is a stress reaction to the captivity or the normal level for this morph. However comparison with two sets of related salamanders that were captured and sampled immediately indicates the red-stripe salamanders were either not stressed from the captivity at all, or their white blood cell distributions had returned to normal after 7 days of captivity. Taken together, our results indicate that lead-phase forms of P. cinereus have higher stress levels than the red-stripe forms, which may be a consequence of their higher exposure to, and/or attacks from, predators. They may also indicate that the lead-phase form is less-suited to captivity than the red-stripe form of this species.
基金Project supported by the National Basic Research Program(973)of China(No.2011CB100805)the Modern Agro-Industry Technology Research System of China(No.nycytx-04-01)the Agricultural Science and Technology Innovation Program(No.ASTIP-IAS12),China
文摘Heat stress affects feed intake, milk production, and endocrine status in dairy cows. The temperaturehumidity index（THI） is employed as an index to evaluate the degree of heat stress in dairy cows. However, it is difficult to ascertain whether THI is the most appropriate measurement of heat stress in dairy cows. This experiment was conducted to investigate the effects of heat stress on serum insulin, adipokines（leptin and adiponectin）, AMP-activated protein kinase（AMPK）, and heat shock signal molecules（heat shock transcription factor（HSF） and heat shock proteins（HSP）） in dairy cows and to research biomarkers to be used for better understanding the meaning of THI as a bioclimatic index. To achieve these objectives, two experiments were performed. The first experiment： eighteen lactating Holstein dairy cows were used. The treatments were： heat stress（HS, THI average=81.7, n=9） and cooling（CL, THI average=53.4, n=9）. Samples of HS were obtained on August 16, 2013, and samples of CL were collected on April 7, 2014 in natural conditions. The second experiment： HS treatment cows（n=9） from the first experiment were fed for 8 weeks from August 16, 2013 to October 12, 2013. Samples for moderate heat stress, mild heat stress, and no heat stress were obtained, respectively, according to the physical alterations of the THI. Results showed that heat stress significantly increased the serum adiponectin, AMPK, HSF, HSP27, HSP70, and HSP90（P〈0.05）. Adiponectin is strongly associated with AMPK. The increases of adiponectin and AMPK may be one of the mechanisms to maintain homeostasis in heat-stressed dairy cows. When heat stress treatment lasted 8 weeks, a higher expression of HSF and HSP70 was observed under moderate heat stress. Serum HSF and HSP70 are sensitive and accurate in heat stress and they could be potential indicators of animal response to heat stress. We recommend serum HSF and HSP70 as meaningful biomarkers to supplement the THI and evaluate moderate heat stress in dairy cows in the future.
