肝硬化患者血清性激素测定的临床意义

肝脏是性激素代谢、降解、转化、清除的主要器管。男性肝硬化患者容易女性化的倾向,早为人们所熟知,其发生机制尚不完全清楚,为此,我们对60例男性肝硬化患者的基础血清性激素进行了测定,了解性激素与肝硬化的关系,并探讨其临床意义。 临床资料和方法 一、临床资料:选择1992年～1995年在本院住院,经病史、临床表现、生化检查及B超、CT等检查确诊为肝硬化的男性患者60例,且一个月内均未应用任何能导致血清性激素增高的药物,年龄为32～79岁(平均54.9岁),其中肝炎后肝硬化43例,酒精性肝硬化11例,血吸虫性肝硬化6例。按Kely分组标准分为2组:肝硬化代偿期组10例,肝硬化失代偿期组50例。伴腹水36例,食道静脉曲张46例,乳房发育8例,肝掌、蜘蛛痣28例,肝功能异常(A/G≤1,ALT、

Study of serum level of sex hormones and expression of their receptors in patients with bronchogenic carcinoma

Objective: To study the serum level of estradiol, progesterone and testosterone (SEL, SPL and STL) and the expression of the receptors of estradiol and progesterone (ER and PR) in 53 cases of bronchogenic carcinoma. Methods: ER and PR in the tissue of the carcinoma were determined with enzyme-linked affinity histochemical method. SEL, SPL and STL were measured with double antibody radioimmunoassay. Results: Most of ER and PR were present in the cytoplasm of the malignant cells (58.2%) and the positive rates of ER and PR were 49.1% and 54.7% respectively. SEL and SPL were significantly higher in the patients with lung cancer than in the subjects of the control groups (P<0.05), no matter whether ER and PR were positive or negative. SEL and SPL were lower in the ER positive, PR positive and both ER and PR positive groups than in the ER negative, PR negative and both ER and PR negative groups. Conclusion: The existence of ER and PR in the patients with bronchogenic carcinoma indicates that the pathogenesis of bronchogenic carcinoma is sex hormone dependent to some extent. ER and SEL are negatively correlated with a correlative coefficient of -1.

Androgen deficiency in elderly men with systolic chronic heart failure

Objective Several previous studies have shown androgens deficiency in men with CHF, and 2 studies on the prognostic significance of serum levels of androgens in CHF patients have yielded conflicting results. The aim of this study was to examine the relationship between serum concentration of testosterone and mortality in men with systolic CHE Methods A total of 175 elderly （age ≥ 60 years） men with CHF were recruited. Total testosterone fiT） and sex hormone binding globulin （SHBG） were measured, and free serum testosterone （eFT） was calculated. The median follow-up time was 1262 days. Results During follow-up 54 （30.9%） patients died. TT and eFF deficiency was found in 21.7% （38/175） and 27.4% （48/175） patients, respectively. Both TT and eFT were inversely associated with LVEF and NT-proBNP （all P〈0.01）. Kaplan-Meier curves for patients in low, medium and high tertiles according to TF and eFT level showed significantly different cumulative survival rate （both P〈0.01 by log-rank test）. However, after adjustment for clinical variables, there were no significant associations between either Tr or eFT levels or survival time （OR=0.97, 95% CI, 0.84-1.12, P--0.28; and OR=0.92, 95% CI, 0.82-1.06,/＇=0.14, respectively）. Conclusion Our study showed that although levels of TT and eFT are commonly decreased in elderly patients with systolic CHF and related to disease severity, they are not independent predictors for mortality

MEASUREMENT OF SERUM GRANULOCYTE COLONY-STIMULATING FACTOR LEVELS IN PATIENTS WITH DIFFERENT PHASE OF INFECTION

Objective To detect the serum granulocyte colony-stimulating factor (G-CSF) levels betweenthe patients with frequently repeated infection ( repeaters) and others (non-repeaters) in different phase of infection.Methods An enzyme-linked immunosorbent assay (ELISA) method was used to detect serum G-CSF levels in 50cases (32 non-repeaters and 18 repeaters) with acute phase of infection. Serum G-CSF levels were detected inrecovery phase in 10 cases. Results Serum G-CSF levels were significantly higher (1429. 97 ±506. 43ng/L) in32 non-repeaters with acute infection. There was a positive correlation between white blood cell count ( WBC) andserum G-CSF level (r =0. 396, P <0. 05). There was also a positive correlation between absolute neutrophil count(ANC) and serum G-CSF level (r =0. 346,P <0. 05). Serum G-CSF levels were higher (98. 62 ±56. 40ng/L) in18 repeaters with acute infection. It was showed that serum G-CSF levels were significantly higher in non-repeatersthan in repeaters with acute phase of infection (P <0. 001). In the meanwhile, the body temperature was signifi-cantly higher in non-repeaters than in repeaters with acute infection (37. 95 ±0. 14 vs 36. 91 ±0. 13 ,P<0. 001). There were no significant differences in age, WBC, ANC, type of bacterial, liver function and renal func-tion (P >0. 05). Serum G-CSF levels in recovery phase of the two groups were below the sensitivity of the assay( <60 ng/L). Conclusion It is suggested that application of recombinant G-CSF may be useful for the patientswith repeated infection.

Induction of follicular luteinization by equine chorionic gonadotropin in cyclic guinea pigs

The effects of equine chorionic gonadotropin （eCG） on follicular development and ovulation in cyclic guinea pigs were investigated by histological and immunohistochemical analyses. Three groups of guinea pigs （n=12） were administrated subcutaneously with saline, 20 or 50 IU of eCG, respectively, on cyclic Day 12 （Day 1=vaginal openings）. Ovaries were collected at 4 and 8 d after administration （6 animals per group each time）. The eCG administration induced significant and distinct morphological changes in the ovaries, as it promoted the luteinization of granulosa cells, but not follicular development. In addition, proliferating cell nuclear antigen （PCNA） and steroidogenic acute regulatory protein （STAR） were immunolocalized specifically in luteinized follicles. Our experiments together indicate that eCG administration can induce follicular luteinization but not superovulation in guinea pigs. The eCG in cyclic guinea pigs functions similar to that of luteinizing hormone （LH）, but not follicle-stimulating hormone （FSH）.