阿血清抗体检测方法在布病监测中的意义风险

目的:探究分析血清抗体检测方法在布病监测中的意义风险。方法:2019年3月-2019年11月,本中心一共获取99例疑似布鲁氏杆菌病患者,进行布鲁氏杆菌病筛查诊断,经患者同意后,采集患者的空腹静脉血,血样分别进行试管凝集试验与虎红平板凝集试验,获取两种试验结果后采用统计学方法进行比较。结果:共有24例确诊布鲁氏杆菌病。试管凝集试验检出阳性20例。阴性79例;虎红平板凝集试验检出阳性24例,阴性75例.两种试验方法的阳性、阴性检出例数比较有差异(P<0.05)。以试管凝集试验为诊断金标准。可发现虎红平板凝集试验的灵敏度85%.特异度91.3%、诊断符合率为100%。结论:试管凝集试验与虎红平板凝集试验均可用于布鲁氏杆菌病监测.均可获得较准确的检测结果,但虎红平板凝集试验可在大面积疫情中应用.更具应用价值。

Comparative Studies on Detection of Antibodies against Infectious Bursal Disease Virus with Test Strips and Agar Gel Immunodiffusion Method

[Objective] This study aimed to compare the detection results of antibodies against infectious bursal disease virus with test strips and agar gel immunodiffusion method. [Method] Antibodies against infectious bursal disease virus in chicken serum were detected using test strips developed in our laboratory, and the results were comparad^with that using traditional agar diffusion method. [Result] The comparative study of the two methods showed that the sensitivity of test strips was eight times over agar gel immunodiffusion; test strips showed higher detection rate in the deter- mination test of 216 clinical samples, with high specificity, easy preservation, and simple and rapid operation, thereby being more suitable for the monitoring of clinical antibodies. [Conclusion] Test strips could replace the existing serological methods, having great promotion and application value in antibody monitoring.