Potency is one of the most important indexes of inactivated vaccines.A number of methods have been established to assay the potency,of which the NIH test and single-dose mouse protection test are the "prescribed ...Potency is one of the most important indexes of inactivated vaccines.A number of methods have been established to assay the potency,of which the NIH test and single-dose mouse protection test are the "prescribed methods".Here,we report a method to semi-quantitatively assay the potency of an inactivated rabies vaccine,which uses fewer animals and takes less time to complete.Depending on the quality requirements of a vaccine(e.g.minimum potency),a rabies reference vaccine is,for example,diluted to the minimum potency,and 50 μL of the dilution is taken to inoculate 10 mice.The same amount of the test rabies vaccine is inoculated into another 10 mice.After two weeks,all mice are bled and serum samples are assayed for viral neutralizing antibody by the fluorescent antibody virus neutralization(FAVN) test.By comparing the median and interquartile range of antibody titers of the reference vaccine with those of the test vaccine,the test vaccine potency can be semi-quantitatively judged as to whether it is in accord with the required quality.The reliability of this method was also confirmed in dogs.The procedure can be recommended for batch potency testing during inactivated rabies vaccine production.展开更多
Objective To detect the serum granulocyte colony-stimulating factor (G-CSF) levels betweenthe patients with frequently repeated infection ( repeaters) and others (non-repeaters) in different phase of infection.Methods...Objective To detect the serum granulocyte colony-stimulating factor (G-CSF) levels betweenthe patients with frequently repeated infection ( repeaters) and others (non-repeaters) in different phase of infection.Methods An enzyme-linked immunosorbent assay (ELISA) method was used to detect serum G-CSF levels in 50cases (32 non-repeaters and 18 repeaters) with acute phase of infection. Serum G-CSF levels were detected inrecovery phase in 10 cases. Results Serum G-CSF levels were significantly higher (1429. 97 ±506. 43ng/L) in32 non-repeaters with acute infection. There was a positive correlation between white blood cell count ( WBC) andserum G-CSF level (r =0. 396, P <0. 05). There was also a positive correlation between absolute neutrophil count(ANC) and serum G-CSF level (r =0. 346,P <0. 05). Serum G-CSF levels were higher (98. 62 ±56. 40ng/L) in18 repeaters with acute infection. It was showed that serum G-CSF levels were significantly higher in non-repeatersthan in repeaters with acute phase of infection (P <0. 001). In the meanwhile, the body temperature was signifi-cantly higher in non-repeaters than in repeaters with acute infection (37. 95 ±0. 14 vs 36. 91 ±0. 13 ,P<0. 001). There were no significant differences in age, WBC, ANC, type of bacterial, liver function and renal func-tion (P >0. 05). Serum G-CSF levels in recovery phase of the two groups were below the sensitivity of the assay( <60 ng/L). Conclusion It is suggested that application of recombinant G-CSF may be useful for the patientswith repeated infection.展开更多
基金the China National"863"Program(Approval No.2011AA10A212)Special Fund for Agro-Scientific Research in the Public Interest(ApprovalNo.201203056)
文摘Potency is one of the most important indexes of inactivated vaccines.A number of methods have been established to assay the potency,of which the NIH test and single-dose mouse protection test are the "prescribed methods".Here,we report a method to semi-quantitatively assay the potency of an inactivated rabies vaccine,which uses fewer animals and takes less time to complete.Depending on the quality requirements of a vaccine(e.g.minimum potency),a rabies reference vaccine is,for example,diluted to the minimum potency,and 50 μL of the dilution is taken to inoculate 10 mice.The same amount of the test rabies vaccine is inoculated into another 10 mice.After two weeks,all mice are bled and serum samples are assayed for viral neutralizing antibody by the fluorescent antibody virus neutralization(FAVN) test.By comparing the median and interquartile range of antibody titers of the reference vaccine with those of the test vaccine,the test vaccine potency can be semi-quantitatively judged as to whether it is in accord with the required quality.The reliability of this method was also confirmed in dogs.The procedure can be recommended for batch potency testing during inactivated rabies vaccine production.
文摘Objective To detect the serum granulocyte colony-stimulating factor (G-CSF) levels betweenthe patients with frequently repeated infection ( repeaters) and others (non-repeaters) in different phase of infection.Methods An enzyme-linked immunosorbent assay (ELISA) method was used to detect serum G-CSF levels in 50cases (32 non-repeaters and 18 repeaters) with acute phase of infection. Serum G-CSF levels were detected inrecovery phase in 10 cases. Results Serum G-CSF levels were significantly higher (1429. 97 ±506. 43ng/L) in32 non-repeaters with acute infection. There was a positive correlation between white blood cell count ( WBC) andserum G-CSF level (r =0. 396, P <0. 05). There was also a positive correlation between absolute neutrophil count(ANC) and serum G-CSF level (r =0. 346,P <0. 05). Serum G-CSF levels were higher (98. 62 ±56. 40ng/L) in18 repeaters with acute infection. It was showed that serum G-CSF levels were significantly higher in non-repeatersthan in repeaters with acute phase of infection (P <0. 001). In the meanwhile, the body temperature was signifi-cantly higher in non-repeaters than in repeaters with acute infection (37. 95 ±0. 14 vs 36. 91 ±0. 13 ,P<0. 001). There were no significant differences in age, WBC, ANC, type of bacterial, liver function and renal func-tion (P >0. 05). Serum G-CSF levels in recovery phase of the two groups were below the sensitivity of the assay( <60 ng/L). Conclusion It is suggested that application of recombinant G-CSF may be useful for the patientswith repeated infection.
