Idiopathic pulmonary arterial hypertension(IPAH) is a rare disease of unknown etiology.The exact pathogenesis of pulmonary arterial hypertension is still not well known.In the past decades,many protein molecules have ...Idiopathic pulmonary arterial hypertension(IPAH) is a rare disease of unknown etiology.The exact pathogenesis of pulmonary arterial hypertension is still not well known.In the past decades,many protein molecules have been found to be in-volved in the development of IPAH.With proteomic techniques,profiling of human plasma proteome becomes more feasible in searching for disease-related markers.In present study,we showed the protein expression profiles of the serum of IPAH and healthy controls after depleting a few high-abundant proteins in serum.Thirteen spots had changed significantly in IPAH com-pared with healthy controls and were identified by LC-MS/MS.Alpha-1-antitrypsin and vitronectin were down-regulated in IPAH and may be valuable candidates for further explorations of their roles in the development of IPAH.展开更多
Carcinogenesis of lung squamous carcinoma is a complex process involving multiple events and steps. At present, there are very few special lung cancer molecular markers for an 'early-stage' diagnosis and progn...Carcinogenesis of lung squamous carcinoma is a complex process involving multiple events and steps. At present, there are very few special lung cancer molecular markers for an 'early-stage' diagnosis and prognosis evaluation. To identify tumor-associated antigens, serological proteome analysis (SERPA) of human lung squamous carcinoma cell line HTB-182 are performed. We characterized sixteen differentially expressed proteins which react with lung squamous carcinoma patient sera while not react with control sera. Some of these candidate lung squamous carcinoma-associated antigens were metabolic enzymes, such as triosephosphatase isomerase (TPIS). Some proteins were involved in the regulation of cell cycle and signal transduction. The results will provide scientific foundation for screening the molecular biomarkers used to diagnose and treat lung squamous carcinoma, as well as to elevate the patient's prognosis and provide new clue for the research of lung squamous carcinogenic mechanism. Thus, SERPA represents a valuable approach for the identification of differentially expressed proteins, which might be used as markers for the diagnosis and prognosis of lung squamous carcinoma.展开更多
基金Project (No. A-007) supported by the Key Medicine Foundation of Zhejiang Province, China
文摘Idiopathic pulmonary arterial hypertension(IPAH) is a rare disease of unknown etiology.The exact pathogenesis of pulmonary arterial hypertension is still not well known.In the past decades,many protein molecules have been found to be in-volved in the development of IPAH.With proteomic techniques,profiling of human plasma proteome becomes more feasible in searching for disease-related markers.In present study,we showed the protein expression profiles of the serum of IPAH and healthy controls after depleting a few high-abundant proteins in serum.Thirteen spots had changed significantly in IPAH com-pared with healthy controls and were identified by LC-MS/MS.Alpha-1-antitrypsin and vitronectin were down-regulated in IPAH and may be valuable candidates for further explorations of their roles in the development of IPAH.
基金Project (2001CB5102) supported by the National Basic Research Program of China Project (30500558) supported by the National Natural Science Foundation of China
文摘Carcinogenesis of lung squamous carcinoma is a complex process involving multiple events and steps. At present, there are very few special lung cancer molecular markers for an 'early-stage' diagnosis and prognosis evaluation. To identify tumor-associated antigens, serological proteome analysis (SERPA) of human lung squamous carcinoma cell line HTB-182 are performed. We characterized sixteen differentially expressed proteins which react with lung squamous carcinoma patient sera while not react with control sera. Some of these candidate lung squamous carcinoma-associated antigens were metabolic enzymes, such as triosephosphatase isomerase (TPIS). Some proteins were involved in the regulation of cell cycle and signal transduction. The results will provide scientific foundation for screening the molecular biomarkers used to diagnose and treat lung squamous carcinoma, as well as to elevate the patient's prognosis and provide new clue for the research of lung squamous carcinogenic mechanism. Thus, SERPA represents a valuable approach for the identification of differentially expressed proteins, which might be used as markers for the diagnosis and prognosis of lung squamous carcinoma.
