AIM:To investigate the protective effects of melatonin on carbon tetrachloride(CCl4)-induced hepatic fibrosis in experimental rats.METHODS:All rats were randomly divided into normal control group,model control group t...AIM:To investigate the protective effects of melatonin on carbon tetrachloride(CCl4)-induced hepatic fibrosis in experimental rats.METHODS:All rats were randomly divided into normal control group,model control group treated with CCl4 for 12 wk,CCl4+NAC group treated with CCl4+NAC(100 mg/kg,i.p.)for 12 wk,CCl4+MEL-1 group treated with CCl4+melatonin(2.5 mg/kg)for 12 wk,CCl4+MEL-2 group treated with CCl4+ melatonin(5.0 mg/kg)for 12 wk,and CCl4+MEL-3 group treated with CCl4+melatonin(10 mg/kg).Rats in the treatment groups were injected subcutaneously with sterile CCl4(3 mL/kg,body weight)in a ratio of 2:3 with olive oil twice a week.Rats in normal control group received hypodermic injection of olive oil at the same dose and frequency as those in treatment groups.At the end of experiment,rats in each group were anesthetized and sacrificed.Hematoxylin and eosin(HE)staining and Van Gieson staining were used to examine changes in liver pathology.Serum activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and protein concentration weremeasured with routine laboratory methods using an autoanalyzer.Hydroxyproline(HYP)content in liver and malondialdehyde(MDA)and glutathione peroxidase(GPx)levels in liver homogenates were assayed by spectrophotometry.Serum hyaluronic acid(HA),laminin(LN),and procollagenⅢN-terminal peptide(PⅢNP)were determined by radioimmunoassay.RESULTS:Pathologic grading showed that the fibrogenesis was much less severe in CCl4+MEL3 group than in model control group(u=2.172,P<0.05),indicating that melatonin(10 mg/kg)can significantly ameliorate CCl4-induced hepatic fibrotic changes.The serum levels of ALT and AST were markedly lower in CCl4+MEL treatment groups(5,10 mg/kg)than in model control group(ALT:286.23 ±121.91 U/L vs 201.15±101.16 U/L and 178.67 ±103.14 U/L,P=0.028,P=0.007;AST:431.00 ±166.35 U/L vs 321.23±162.48 U/L and 292.42 ±126.23 U/L,P=0.043,P=0.013).Similarly,the serum laminin(LN)and hyaluronic acid(HA)levels and hydroxyproline(HYP)contents in liver were significantly lower in CCl4+MEL-3 group(10 mg/kg)than in model control group(LN:45.89±11.71μg/L vs 55.26± 12.30μg/L,P=0.012;HA:135.71±76.03μg/L vs 201.10±68.46μg/L,P=0.020;HYP:0.42±0.08 mg/g tissue vs 0.51±0.07 mg/g tissue,P=0.012).Moreover,treatment with melatonin(5,10 mg/kg)significantly reduced the MDA content and increased the GPx activity in liver homogenates compared with model control group(MDA:7.89±1.49 noml/mg prot vs 6.29±1.42 noml/mg prot and 6.25±2.27 noml/mg prot,respectively,P=0.015,P=0.015;GPx:49.13± 8.72 U/mg prot vs 57.38±7.65 U/mg prot and 61.39± 13.15 U/mg prot,respectively,P=0.035,P=0.003).CONCLUSION:Melatonin can ameliorate CCl4-induced hepatic fibrosis in rats.The protective effect of melatonin on hepatic fibrosis may be related to its antioxidant activities.展开更多
The sera of 180 human samples were tested for the presence of antibodies against Borrelia burgdorferi using the ELISA （enzyme-linked immunosorbent assay） technique. Out of 180 sero-samples, 46 （25.55%） were positi...The sera of 180 human samples were tested for the presence of antibodies against Borrelia burgdorferi using the ELISA （enzyme-linked immunosorbent assay） technique. Out of 180 sero-samples, 46 （25.55%） were positive. Females of the age range 18-35 years had the highest rate of sero-positive samples 14 （38.88%）, while the highest percentage of sero-negative samples was found in males of the age range 50-80 years. The other sero-positive samples were： 6 （26.08%）, 6 （25%） and 3 （11.53%） in males of ages between 18-35, 35-50 and 50-80 years, respectively, and 11 （29.72%） and 6 （17.64%） in females in the age ranges 35-50 and 50-80 years, respectively. The mean concentration of Anti- B. burgdorferi antibody was higher （16.7 U/mL） when compared with mean concentration of normal value （5.5 U/mL）, P 〈 0.001.展开更多
Objective： To evaluate the clinical significance of quantitative determination of serum lactic dehydrogenase （LDH） and beta 2-microgtobulin （β2-MG） in patients with non-Hodgkin＇s lymphoma （NHL）. Methods： Dat...Objective： To evaluate the clinical significance of quantitative determination of serum lactic dehydrogenase （LDH） and beta 2-microgtobulin （β2-MG） in patients with non-Hodgkin＇s lymphoma （NHL）. Methods： Date of the 65 patients from 2003 to 2004 were retrospectively analyzed, relationship between LDH and β2-MG activities and tumor phase, effect of treat- ment as well as prognosis were studied. Results： The levels of LDH and β2-MG in the advanced stage, B group and bone marrow involvement were obviously higher than their comparison counterpart and the difference was significant （P 〈 0.05）. There was no significant difference between the high LDH group and normal LDH group in the two-year survival rate. But the difference in the four-year survival rate was significance. Conclusion： The level of LDH and β2-MG are valuable for clinical stage, prognosis and evaluation of therapeutic response in patients with NHL.展开更多
基金Supported by The Natural Science Foundation of Anhui Province No.01043904the Natural Science Research Project of Colleges and Universities of Anhui Province No.KJ2007B146
文摘AIM:To investigate the protective effects of melatonin on carbon tetrachloride(CCl4)-induced hepatic fibrosis in experimental rats.METHODS:All rats were randomly divided into normal control group,model control group treated with CCl4 for 12 wk,CCl4+NAC group treated with CCl4+NAC(100 mg/kg,i.p.)for 12 wk,CCl4+MEL-1 group treated with CCl4+melatonin(2.5 mg/kg)for 12 wk,CCl4+MEL-2 group treated with CCl4+ melatonin(5.0 mg/kg)for 12 wk,and CCl4+MEL-3 group treated with CCl4+melatonin(10 mg/kg).Rats in the treatment groups were injected subcutaneously with sterile CCl4(3 mL/kg,body weight)in a ratio of 2:3 with olive oil twice a week.Rats in normal control group received hypodermic injection of olive oil at the same dose and frequency as those in treatment groups.At the end of experiment,rats in each group were anesthetized and sacrificed.Hematoxylin and eosin(HE)staining and Van Gieson staining were used to examine changes in liver pathology.Serum activities of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and protein concentration weremeasured with routine laboratory methods using an autoanalyzer.Hydroxyproline(HYP)content in liver and malondialdehyde(MDA)and glutathione peroxidase(GPx)levels in liver homogenates were assayed by spectrophotometry.Serum hyaluronic acid(HA),laminin(LN),and procollagenⅢN-terminal peptide(PⅢNP)were determined by radioimmunoassay.RESULTS:Pathologic grading showed that the fibrogenesis was much less severe in CCl4+MEL3 group than in model control group(u=2.172,P<0.05),indicating that melatonin(10 mg/kg)can significantly ameliorate CCl4-induced hepatic fibrotic changes.The serum levels of ALT and AST were markedly lower in CCl4+MEL treatment groups(5,10 mg/kg)than in model control group(ALT:286.23 ±121.91 U/L vs 201.15±101.16 U/L and 178.67 ±103.14 U/L,P=0.028,P=0.007;AST:431.00 ±166.35 U/L vs 321.23±162.48 U/L and 292.42 ±126.23 U/L,P=0.043,P=0.013).Similarly,the serum laminin(LN)and hyaluronic acid(HA)levels and hydroxyproline(HYP)contents in liver were significantly lower in CCl4+MEL-3 group(10 mg/kg)than in model control group(LN:45.89±11.71μg/L vs 55.26± 12.30μg/L,P=0.012;HA:135.71±76.03μg/L vs 201.10±68.46μg/L,P=0.020;HYP:0.42±0.08 mg/g tissue vs 0.51±0.07 mg/g tissue,P=0.012).Moreover,treatment with melatonin(5,10 mg/kg)significantly reduced the MDA content and increased the GPx activity in liver homogenates compared with model control group(MDA:7.89±1.49 noml/mg prot vs 6.29±1.42 noml/mg prot and 6.25±2.27 noml/mg prot,respectively,P=0.015,P=0.015;GPx:49.13± 8.72 U/mg prot vs 57.38±7.65 U/mg prot and 61.39± 13.15 U/mg prot,respectively,P=0.035,P=0.003).CONCLUSION:Melatonin can ameliorate CCl4-induced hepatic fibrosis in rats.The protective effect of melatonin on hepatic fibrosis may be related to its antioxidant activities.
文摘The sera of 180 human samples were tested for the presence of antibodies against Borrelia burgdorferi using the ELISA （enzyme-linked immunosorbent assay） technique. Out of 180 sero-samples, 46 （25.55%） were positive. Females of the age range 18-35 years had the highest rate of sero-positive samples 14 （38.88%）, while the highest percentage of sero-negative samples was found in males of the age range 50-80 years. The other sero-positive samples were： 6 （26.08%）, 6 （25%） and 3 （11.53%） in males of ages between 18-35, 35-50 and 50-80 years, respectively, and 11 （29.72%） and 6 （17.64%） in females in the age ranges 35-50 and 50-80 years, respectively. The mean concentration of Anti- B. burgdorferi antibody was higher （16.7 U/mL） when compared with mean concentration of normal value （5.5 U/mL）, P 〈 0.001.
文摘Objective： To evaluate the clinical significance of quantitative determination of serum lactic dehydrogenase （LDH） and beta 2-microgtobulin （β2-MG） in patients with non-Hodgkin＇s lymphoma （NHL）. Methods： Date of the 65 patients from 2003 to 2004 were retrospectively analyzed, relationship between LDH and β2-MG activities and tumor phase, effect of treat- ment as well as prognosis were studied. Results： The levels of LDH and β2-MG in the advanced stage, B group and bone marrow involvement were obviously higher than their comparison counterpart and the difference was significant （P 〈 0.05）. There was no significant difference between the high LDH group and normal LDH group in the two-year survival rate. But the difference in the four-year survival rate was significance. Conclusion： The level of LDH and β2-MG are valuable for clinical stage, prognosis and evaluation of therapeutic response in patients with NHL.
