地高辛掺入法检测血清HCV-RNA含量临床应用价值的研究

目的研究采用地高辛掺入法对血清HCV—RNA进行定量检测的临床应用价值。方法在逆转录PCR的过程中加入地高辛标记的dVTP掺入到PCR产物中，然后应用微孔板杂交技术进行ELISA测定，结合标准参考样本而达到定量目的。结果（1）感染HCV后肝炎后肝硬化组血清HCV—RNA含量显著高于肝炎组；（2）血清HCV—RNA含量高低对干扰素的治疗效果有明显影响，HCV—RNA含量低者干扰素治疗效果好于HCV—RNA含量高者。结论地高辛掺入法检测血清HCV—RNA含量，在临床有广阔的应用价值。

RELATIONSHIP BETWEEN LOAD OF HCV RNA AND ANTIBODIES AGAINST REGIONAL SPECIFIC PROTEINS OF HCV IN SERUM

Objective To study whether HCV RNA load is related to the antibodies against regional spe-cific proteins of HCV. Methods 69 serum samples were detected for HCV RNA load by Real time fluorescent PCR. Anti-HCV and the antibodies against regional specific proteins of HCV were detected by ELISA. Results The positive rates of anti-C and anti-NS3 were remarkably higher than those of anti-NS4 and anti-NS5. We divided the serum samples into two groups on the basis of load of HCV RNA, there was no statistical significance between A and B group in the positive rate as well as the antibody reactivity of HCV anti-C, anti-NS3, anti-NS4 and anti-NS5. From the distribution of different combined patterns, we could conclude that the pattern IV was superior to others in both of the two groups. Conclusion There are remarkable differences in different antibodies against the regional specific proteins of HCV. The positive rates and the antibody reactivity of anti-C, anti-NS3, anti-NS4, anti-NS5 are not related to the HCV viral load. The EfA-3 which conclude C, NS3, NS4, NS5 proteins could enhance the sensitivity of detection HCV.

双环醇治疗慢性丙型病毒性肝炎的疗效观察

目的观察双环醇对慢性丙型病毒性肝炎的临床疗效。方法对40例慢性丙型肝炎患者随机分为治疗组、对照组各20例,治疗组予双环醇25mg口服,1d3次。对照组予维生素B、C,肝太乐等一般护肝药口服,疗程6个月。结果治疗组治疗第1、3个月末血清谷丙转氨酶(ALT)复常率高于对照组(P<0.05);治疗组患者治疗前后血清HCV-RNA对数值下降较对照组有统计学意义(P<0.05)。结论双环醇治疗慢性丙型病毒性肝炎降酶效果明显,且在人体内可能有协助抑制HCV增殖的作用。