Activities of hexokinase(HK),pyruvate kinase(PK) and levels of HSP70 were measured to evaluate the response of Litopenaeus vannamei to rapid temperature changes under controlled laboratory conditions.Shrimps were subj...Activities of hexokinase(HK),pyruvate kinase(PK) and levels of HSP70 were measured to evaluate the response of Litopenaeus vannamei to rapid temperature changes under controlled laboratory conditions.Shrimps were subjected to a quick temperature change from 27℃ to 17℃ for the summer case(Cold temperature treatment),or from 17℃ to 27℃ for the winter case(Warm temperature treatment).After 0.5,1,3,6,12,24,48,and 72 h of exposure time,shrimps were sampled and prepared for further analysis.The results showed that the effect of acute temperature changes on activities of HK was significant.Patterns of variations of the two glycolytic enzymes suggested that enzymes in the glycolysis cycle could adjust their activities to meet the acute temperature change.The HSP70 level increased in both cold and warm temperature treatments,suggesting that the rapid temperature changes activated the process of body's self-protection.But the difference in expression peak of HSP70 might be related to the different body size and the higher thermal sensitivity to temperature increase than to temperature decrease of L.vannamei.展开更多
High temperature influences the homeostasis of fish.We investigated the effects of elevated temperature on tissues of Japanese flounder {Paralichthys olivaceus) by analyzing the histology and heat shock protein 70(hsp...High temperature influences the homeostasis of fish.We investigated the effects of elevated temperature on tissues of Japanese flounder {Paralichthys olivaceus) by analyzing the histology and heat shock protein 70(hsp70) expression of fish reared in warm conditions.In this study,temperature was increased at l±0.5℃/day starting at 24±0.5℃,and was kept at that temperature for 5 days before the next rise.After raising temperature at the rate up to 32±0.5℃,tissue samples from midgut,spleen,stomach,liver,muscle,gill,heart,trunk kidney and brain were collected for histological analysis and mRNA assay.Almost all the tissues showed changes in morphological structure and hsp70 level at 32±0.5℃.Histological assessment of the tissues indicated that the gill had the most serious damage,including highly severe epithelial lifting and edema,curved tips and hyperemia at the ending of the lamellars,desquamation and necrosis.The next most severe damage was found in liver and kidney.The hsp70 levels in all the tissues first increased and then decreased.The gut,stomach,muscle,heart,and brain had the highest expressions in 6 h,whereas the spleen,liver,gill and kidney had the highest expressions in 2 h.Therefore,tissues with the most significant lesions(especially gill and liver) responded much earlier(2 h) in hsp70 expression than other tissues,and these tissues demonstrated the most marked histological disruption and elevated mRNA levels,making them ideal candidates for further studies on the thermal physiology of this species.展开更多
目的探讨康复新液联合健康教育干预治疗慢性糜烂性胃炎的临床效果。方法将收治的慢性糜烂性胃炎患者148例随机分为对照组和观察组,每组74例。对照组给予康复新液治疗,观察组在对照组基础上加强健康教育指导,比较两组患者临床疗效、临床...目的探讨康复新液联合健康教育干预治疗慢性糜烂性胃炎的临床效果。方法将收治的慢性糜烂性胃炎患者148例随机分为对照组和观察组,每组74例。对照组给予康复新液治疗,观察组在对照组基础上加强健康教育指导,比较两组患者临床疗效、临床症状积分和血清热休克蛋白(HSP)70 m RNA水平。结果观察组临床有效率为87.84%,显著高于对照组的68.92%(P<0.05);治疗后两组患者反酸、嗳气和上腹痛症状积分显著低于治疗前(P<0.05),且观察组显著低于对照组(P<0.05);两组患者治疗后HSP70 m RNA水平显著高于治疗前,且观察组显著高于对照组(P<0.05)。结论康复新液联合健康教育干预治疗慢性糜烂性胃炎效果显著,可有效改善临床症状,诱导HSP70表达,优于单用康复新液治疗。展开更多
基金supported by National Natural Science Foundation of China (Grant No 30571441)the Key Project of the National Scientific and Technical Supporting Programs funded by the Ministry of Science and Technology of China (Grant No 2006BAD09A07)the Major State Basic Research Development Program of China (973 Program, No 2009CB 118706)
文摘Activities of hexokinase(HK),pyruvate kinase(PK) and levels of HSP70 were measured to evaluate the response of Litopenaeus vannamei to rapid temperature changes under controlled laboratory conditions.Shrimps were subjected to a quick temperature change from 27℃ to 17℃ for the summer case(Cold temperature treatment),or from 17℃ to 27℃ for the winter case(Warm temperature treatment).After 0.5,1,3,6,12,24,48,and 72 h of exposure time,shrimps were sampled and prepared for further analysis.The results showed that the effect of acute temperature changes on activities of HK was significant.Patterns of variations of the two glycolytic enzymes suggested that enzymes in the glycolysis cycle could adjust their activities to meet the acute temperature change.The HSP70 level increased in both cold and warm temperature treatments,suggesting that the rapid temperature changes activated the process of body's self-protection.But the difference in expression peak of HSP70 might be related to the different body size and the higher thermal sensitivity to temperature increase than to temperature decrease of L.vannamei.
基金Supported by the Modern Agro-Industry Technology Research System of China(No.nycytx-50)the Key Innovation Program of Chinese Academy of Sciences,the Experiment and Demonstration of Scientific and Technical Innovation on Modern Ecological Ocean Agriculture(No.KSC2-EW-B-3)+4 种基金the Transformation Fund for Agricultural Science and Technology Achievements(No.2013GB2C600263)the Science Technology R&D Project of Shandong Province(No.2011GHy 11530)the Shandong Province Agricultural Seed Project(No.2014-2016)the Jiangsu Provincial Natural Science Foundation of China(No.BK2012222)the Fundamental Research Project of Technology Program of Qingdao,China(Nos.12-l-4-8(6)-jch,12-l-4-8-(7)-jch,12-4-1-51-hy)
文摘High temperature influences the homeostasis of fish.We investigated the effects of elevated temperature on tissues of Japanese flounder {Paralichthys olivaceus) by analyzing the histology and heat shock protein 70(hsp70) expression of fish reared in warm conditions.In this study,temperature was increased at l±0.5℃/day starting at 24±0.5℃,and was kept at that temperature for 5 days before the next rise.After raising temperature at the rate up to 32±0.5℃,tissue samples from midgut,spleen,stomach,liver,muscle,gill,heart,trunk kidney and brain were collected for histological analysis and mRNA assay.Almost all the tissues showed changes in morphological structure and hsp70 level at 32±0.5℃.Histological assessment of the tissues indicated that the gill had the most serious damage,including highly severe epithelial lifting and edema,curved tips and hyperemia at the ending of the lamellars,desquamation and necrosis.The next most severe damage was found in liver and kidney.The hsp70 levels in all the tissues first increased and then decreased.The gut,stomach,muscle,heart,and brain had the highest expressions in 6 h,whereas the spleen,liver,gill and kidney had the highest expressions in 2 h.Therefore,tissues with the most significant lesions(especially gill and liver) responded much earlier(2 h) in hsp70 expression than other tissues,and these tissues demonstrated the most marked histological disruption and elevated mRNA levels,making them ideal candidates for further studies on the thermal physiology of this species.
文摘目的探讨康复新液联合健康教育干预治疗慢性糜烂性胃炎的临床效果。方法将收治的慢性糜烂性胃炎患者148例随机分为对照组和观察组,每组74例。对照组给予康复新液治疗,观察组在对照组基础上加强健康教育指导,比较两组患者临床疗效、临床症状积分和血清热休克蛋白(HSP)70 m RNA水平。结果观察组临床有效率为87.84%,显著高于对照组的68.92%(P<0.05);治疗后两组患者反酸、嗳气和上腹痛症状积分显著低于治疗前(P<0.05),且观察组显著低于对照组(P<0.05);两组患者治疗后HSP70 m RNA水平显著高于治疗前,且观察组显著高于对照组(P<0.05)。结论康复新液联合健康教育干预治疗慢性糜烂性胃炎效果显著,可有效改善临床症状,诱导HSP70表达,优于单用康复新液治疗。
