Objective:Evaluation of a polyclonal and a monoclonal Helicobacter pylori stool antigen test(HpSAT)for the detection of Helicobacter pylori(HP)infection in children.Methods:43 children underwent a 13C-urea breath test...Objective:Evaluation of a polyclonal and a monoclonal Helicobacter pylori stool antigen test(HpSAT)for the detection of Helicobacter pylori(HP)infection in children.Methods:43 children underwent a 13C-urea breath test(13C UBT)and a HpSAT for the detection of HP.A child was considered HP positive if the 13C UBT was positive.Two HpSATs were tested:the “Premier Platinum HpSA”test(PP HpSAT)(polyclonal enzyme immunoassay)and the “ImmunoCard STAT! HpSA”test(ICS HpSAT)(one-step immunochromatographic assay).Results:The mean age of the children was 8.9 y(range 3.5-17.5 y).Of the 43 children,18(41.9%)were HP positive and 25(58.1%)were HP negative.The PP HpSAT showed a sensitivity of 94.4%and a specificity of 100.0%.With the ICS HpSAT,equivocal results occurred in 5/42(11.9%)of the tests due to a problem with the visual interpretation of the change in colour of the test-line.The ICS HpSAT had a sensitivity of 100.0%and a specificity of 76.0%when the test was considered positive in case of any change of colour of the test-line,which is the correct practice according to the manufacturer.The ICS HpSAT had a sensitivity of 100.0%and a specificity of 96.0%when the test was considered positive only in case of a “significant change of colour”of the test-line.Conclusion:Compared to the 13C UBT,the PP HpSAT shows a comparably good sensitivity and specificity,the ICS HpSAT has a comparably good sensitivity but lower specificity due to a high percentage of equivocal results when the test is used according to the manufacturer’s instructions,and the ICS HpSAT has a comparably good sensitivity and specificity when a weakly positive test is considered negative for the diagnosis of HP infection in children.The ICS HpSAT is easy to perform with results available within 10 min,and is therefore of particular interest in ambulatory medicine.展开更多
We assessed the sensitivity and specificity of H. pylori IgG and IgA with a commercial immunoassay performed in Chile and a second non-commercial immunoassay performed in a reference laboratory in the United States, i...We assessed the sensitivity and specificity of H. pylori IgG and IgA with a commercial immunoassay performed in Chile and a second non-commercial immunoassay performed in a reference laboratory in the United States, in serum of 80 children and adults referred for gastrointestinal endoscopies in a developing country. Overall, 56%of the patients were infected with H. pylori based on rapid urease test and staining techniques on gastric biopsies. When Receiver Operator Curves (ROC) were developed, the sensitivity and specificity were similar for IgG and IgA. Both immunoassays exhibited better specificity, positive and negative predictive value (NPV) in children than in adults when cut-off values were corrected according to the local population than when they were assessed using the cut-off values pre-defined in other populations. These results underline the need to establish more precise cut-off values corrected in the local populations where assessments of antibodies as diagnostic markers of H. pylori infection are planning.展开更多
沙眼衣原体(Chlamydiatraehomatis，CT)所引起的泌尿生殖道感染，近20年在西方国家己成为最常见的一种性传播疾病(Sexually trans mired disease，STD)，远远超过淋球菌及其他STD感染。在工业化社会，梅毒和淋病的发病率已有所下降，但...沙眼衣原体(Chlamydiatraehomatis，CT)所引起的泌尿生殖道感染，近20年在西方国家己成为最常见的一种性传播疾病(Sexually trans mired disease，STD)，远远超过淋球菌及其他STD感染。在工业化社会，梅毒和淋病的发病率已有所下降，但是其他STD如衣原体，其发病率之高，危害之大，已成为严重的公共卫生问题。展开更多
文摘Objective:Evaluation of a polyclonal and a monoclonal Helicobacter pylori stool antigen test(HpSAT)for the detection of Helicobacter pylori(HP)infection in children.Methods:43 children underwent a 13C-urea breath test(13C UBT)and a HpSAT for the detection of HP.A child was considered HP positive if the 13C UBT was positive.Two HpSATs were tested:the “Premier Platinum HpSA”test(PP HpSAT)(polyclonal enzyme immunoassay)and the “ImmunoCard STAT! HpSA”test(ICS HpSAT)(one-step immunochromatographic assay).Results:The mean age of the children was 8.9 y(range 3.5-17.5 y).Of the 43 children,18(41.9%)were HP positive and 25(58.1%)were HP negative.The PP HpSAT showed a sensitivity of 94.4%and a specificity of 100.0%.With the ICS HpSAT,equivocal results occurred in 5/42(11.9%)of the tests due to a problem with the visual interpretation of the change in colour of the test-line.The ICS HpSAT had a sensitivity of 100.0%and a specificity of 76.0%when the test was considered positive in case of any change of colour of the test-line,which is the correct practice according to the manufacturer.The ICS HpSAT had a sensitivity of 100.0%and a specificity of 96.0%when the test was considered positive only in case of a “significant change of colour”of the test-line.Conclusion:Compared to the 13C UBT,the PP HpSAT shows a comparably good sensitivity and specificity,the ICS HpSAT has a comparably good sensitivity but lower specificity due to a high percentage of equivocal results when the test is used according to the manufacturer’s instructions,and the ICS HpSAT has a comparably good sensitivity and specificity when a weakly positive test is considered negative for the diagnosis of HP infection in children.The ICS HpSAT is easy to perform with results available within 10 min,and is therefore of particular interest in ambulatory medicine.
文摘We assessed the sensitivity and specificity of H. pylori IgG and IgA with a commercial immunoassay performed in Chile and a second non-commercial immunoassay performed in a reference laboratory in the United States, in serum of 80 children and adults referred for gastrointestinal endoscopies in a developing country. Overall, 56%of the patients were infected with H. pylori based on rapid urease test and staining techniques on gastric biopsies. When Receiver Operator Curves (ROC) were developed, the sensitivity and specificity were similar for IgG and IgA. Both immunoassays exhibited better specificity, positive and negative predictive value (NPV) in children than in adults when cut-off values were corrected according to the local population than when they were assessed using the cut-off values pre-defined in other populations. These results underline the need to establish more precise cut-off values corrected in the local populations where assessments of antibodies as diagnostic markers of H. pylori infection are planning.
文摘沙眼衣原体(Chlamydiatraehomatis，CT)所引起的泌尿生殖道感染，近20年在西方国家己成为最常见的一种性传播疾病(Sexually trans mired disease，STD)，远远超过淋球菌及其他STD感染。在工业化社会，梅毒和淋病的发病率已有所下降，但是其他STD如衣原体，其发病率之高，危害之大，已成为严重的公共卫生问题。
