目的观察恒温毯保温在重型脑外伤(sTBI)患者血管内低温治疗中的效果。方法回顾性分析2013年1月至2018年12月行血管内低温治疗的sTBI患者112例,根据是否采用36.5℃恒温保温毯进行体表保温,将患者分为对照组(n=58)和观察组(n=54)。采用床...目的观察恒温毯保温在重型脑外伤(sTBI)患者血管内低温治疗中的效果。方法回顾性分析2013年1月至2018年12月行血管内低温治疗的sTBI患者112例,根据是否采用36.5℃恒温保温毯进行体表保温,将患者分为对照组(n=58)和观察组(n=54)。采用床边寒战评估量表(BSAS)评估患者寒战发生率和级别,记录两组低温治疗中抗寒战药物用量、凝血功能、颅内压,以及治疗后1个月、6个月、12个月后扩展版格拉斯哥预后量表(GOSE)评分和病死率。结果观察组寒战发生率和寒战严重程度均明显低于对照组(χ2=16.212, P <0.01);观察组抗寒战药物用量少于对照组(t> 1.269, P <0.05)。观察组低温治疗6 h时,高凝状态开始改善,12 h时趋于稳定;对照组低温治疗12 h时,高凝状态开始改善,24 h时趋于稳定。观察组低温治疗6 h后,颅内压低于对照组。观察组治疗后1个月、6个月、12个月后,GOSE评分和病死率均低于对照组(t> 1.168, P <0.05)。结论采用恒温毯保温可有效降低sTBI患者血管内低温治疗时寒战发生率和严重程度,改善高凝血症,降低颅内压,改善预后。展开更多
We extracted marine low-temperature lysozyme (MLTL),a novel lysozyme,from a marine microorganism through fermentation.Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity thr...We extracted marine low-temperature lysozyme (MLTL),a novel lysozyme,from a marine microorganism through fermentation.Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis.In this study,we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro.Using zebrafish embryos as an in vivo study model,we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs.An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation,migration and tube formation of HUVECs in a dose-dependent manner.Interestingly,assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs.Moreover,further study demonstrated that the disruption of intracellular Ca2+ homeostasis may play an important role in MLTL induced apoptosis of HUVECs.Taken together,the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca2+ levels.The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.展开更多
文摘目的观察恒温毯保温在重型脑外伤(sTBI)患者血管内低温治疗中的效果。方法回顾性分析2013年1月至2018年12月行血管内低温治疗的sTBI患者112例,根据是否采用36.5℃恒温保温毯进行体表保温,将患者分为对照组(n=58)和观察组(n=54)。采用床边寒战评估量表(BSAS)评估患者寒战发生率和级别,记录两组低温治疗中抗寒战药物用量、凝血功能、颅内压,以及治疗后1个月、6个月、12个月后扩展版格拉斯哥预后量表(GOSE)评分和病死率。结果观察组寒战发生率和寒战严重程度均明显低于对照组(χ2=16.212, P <0.01);观察组抗寒战药物用量少于对照组(t> 1.269, P <0.05)。观察组低温治疗6 h时,高凝状态开始改善,12 h时趋于稳定;对照组低温治疗12 h时,高凝状态开始改善,24 h时趋于稳定。观察组低温治疗6 h后,颅内压低于对照组。观察组治疗后1个月、6个月、12个月后,GOSE评分和病死率均低于对照组(t> 1.168, P <0.05)。结论采用恒温毯保温可有效降低sTBI患者血管内低温治疗时寒战发生率和严重程度,改善高凝血症,降低颅内压,改善预后。
基金Supported by the National High Technology Research and Development Program (863 Program) (No.2003AA625070)the Research Fund Program of Qingdao University (No.20051102)
文摘We extracted marine low-temperature lysozyme (MLTL),a novel lysozyme,from a marine microorganism through fermentation.Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis.In this study,we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro.Using zebrafish embryos as an in vivo study model,we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs.An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation,migration and tube formation of HUVECs in a dose-dependent manner.Interestingly,assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs.Moreover,further study demonstrated that the disruption of intracellular Ca2+ homeostasis may play an important role in MLTL induced apoptosis of HUVECs.Taken together,the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca2+ levels.The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.
