Objective: The aim of this study was to observe the effect of radiation on micro-vessel density (MVD) and vascular endothelial growth factor (VEGF) expression in an transplanted tumor of human pancreatic cancer in nud...Objective: The aim of this study was to observe the effect of radiation on micro-vessel density (MVD) and vascular endothelial growth factor (VEGF) expression in an transplanted tumor of human pancreatic cancer in nude mice and to explore the role of radiotherapy on pancreatic cancer angiogenesis. Methods: After vaccinated pancreatic cancer PANC-1 cells, 20 nude mice were randomly divided into radiation group and control group. Radiation group received the radiation (dose of 20 Gy) and were killed 5 days later. The MVD and VEGF expression were determined by immunohistochemistry and then compared with the control group. Results: The number of MVD in the radiation group was significantly lower than that of the control group (8.30 ± 4.55 vs. 13.60 ± 4.28, P < 0.01). The optical density (OD) of VEGF in the radiation group was higher (2.11 ± 0.54 vs. 1.32 ± 0.61, P < 0.05) compared with the control group. Conclusion: The radiotherapy can reduce the number of vessels and increase the VEGF expression of human pancreatic cancer transplanted tumor in nude mice.展开更多
Objective： The aim of our study was to investigate whether Jinshuixian has the abilities of inhibiting tumor growth and radio-sensitivity effect. Methods： Cultured lung A549 ceils were randomly divided into 6 groups...Objective： The aim of our study was to investigate whether Jinshuixian has the abilities of inhibiting tumor growth and radio-sensitivity effect. Methods： Cultured lung A549 ceils were randomly divided into 6 groups： normal control group （NC）, the Jinshuixian group （JSX）, radiotherapy （RT）, JSX for the first day and the next day followed by RT group （JSX~ RT）, RT for the first day and the next day followed by JSX group （RT→JSX） and RT ＋ JSX concomitantly group （JSX ＋ RT）. MTT was applied to measure the cell viability, RT-PCR and ELISA were used to test the expression of mRNA and protein of VEGF. Results： The proliferation of A549 cells were inhibited in JSX and combined groups and the inhibiting effects were time dependent. The expression of VEGF in RT group was increased, however, VEGF in JSX and combination groups were largely decreased over time when compared to NC group. Results in JSX→RT, RT→JSX and JSX ＋ RT groups did not achieve significantly differences. Conclusion： JSX has the ability of anti-tumor growth in vitro accompanied down-regulating of VEGF, especially when combined with radiotherapy, and its effect is time-dependent. However, more studies in vivo and in vitro are needed to further supporting these effects.展开更多
In this paper, we explore the diagnosis and treatment of hemangiopedcytoma （HPC）. A rare case of HPC in the vertebral canal of thoracic segments is reported, and the clinical features as well as treatment approaches...In this paper, we explore the diagnosis and treatment of hemangiopedcytoma （HPC）. A rare case of HPC in the vertebral canal of thoracic segments is reported, and the clinical features as well as treatment approaches of similar cases in the literature are discussed. In the present case, we operatively resected the tumor and performed postoperative radiation therapy, with good treatment results.展开更多
基金Supported by a grant of Cross-class Science Foundation of Xi'an Jiaotong University (No. 08143073)
文摘Objective: The aim of this study was to observe the effect of radiation on micro-vessel density (MVD) and vascular endothelial growth factor (VEGF) expression in an transplanted tumor of human pancreatic cancer in nude mice and to explore the role of radiotherapy on pancreatic cancer angiogenesis. Methods: After vaccinated pancreatic cancer PANC-1 cells, 20 nude mice were randomly divided into radiation group and control group. Radiation group received the radiation (dose of 20 Gy) and were killed 5 days later. The MVD and VEGF expression were determined by immunohistochemistry and then compared with the control group. Results: The number of MVD in the radiation group was significantly lower than that of the control group (8.30 ± 4.55 vs. 13.60 ± 4.28, P < 0.01). The optical density (OD) of VEGF in the radiation group was higher (2.11 ± 0.54 vs. 1.32 ± 0.61, P < 0.05) compared with the control group. Conclusion: The radiotherapy can reduce the number of vessels and increase the VEGF expression of human pancreatic cancer transplanted tumor in nude mice.
文摘Objective： The aim of our study was to investigate whether Jinshuixian has the abilities of inhibiting tumor growth and radio-sensitivity effect. Methods： Cultured lung A549 ceils were randomly divided into 6 groups： normal control group （NC）, the Jinshuixian group （JSX）, radiotherapy （RT）, JSX for the first day and the next day followed by RT group （JSX~ RT）, RT for the first day and the next day followed by JSX group （RT→JSX） and RT ＋ JSX concomitantly group （JSX ＋ RT）. MTT was applied to measure the cell viability, RT-PCR and ELISA were used to test the expression of mRNA and protein of VEGF. Results： The proliferation of A549 cells were inhibited in JSX and combined groups and the inhibiting effects were time dependent. The expression of VEGF in RT group was increased, however, VEGF in JSX and combination groups were largely decreased over time when compared to NC group. Results in JSX→RT, RT→JSX and JSX ＋ RT groups did not achieve significantly differences. Conclusion： JSX has the ability of anti-tumor growth in vitro accompanied down-regulating of VEGF, especially when combined with radiotherapy, and its effect is time-dependent. However, more studies in vivo and in vitro are needed to further supporting these effects.
文摘In this paper, we explore the diagnosis and treatment of hemangiopedcytoma （HPC）. A rare case of HPC in the vertebral canal of thoracic segments is reported, and the clinical features as well as treatment approaches of similar cases in the literature are discussed. In the present case, we operatively resected the tumor and performed postoperative radiation therapy, with good treatment results.
