Objective: An adequate vascularization in the ovary is important for optimal oocyte maturation. By secreting vascular endothelial growth factor (VEGF) granulosa cells promote blood vessel growth. In addition, they par...Objective: An adequate vascularization in the ovary is important for optimal oocyte maturation. By secreting vascular endothelial growth factor (VEGF) granulosa cells promote blood vessel growth. In addition, they paracrinely reduce the production of VEGF antagonist sVEGFR- 1 (soluble VEGF receptor 1) in endothelial cells. In the present study we examined the influence of smoking on the sVEGFR- 1 concentration in human follicular fluid and on the paracrine activity of granulosa cells. Material and Methods: Follicular fluid samples were obtained during oocyte retrieval from 50 smoking and 50 non- smoking women undergoing in- vitro fertilization treatment. Follicular fluids were used either directly for sVEGFR- 1 quantification or added to endothelial cell cultures to determine the soluble receptor concentration in culture supernatant after four days’ incubation. Results: In this study we were able to show that smokers have a distinctly higher concentration of soluble VEGF receptor 1 in follicular fluid than non- smokers (499.6± 77.7 pg/ml versus 159.2± 29.8 pg/ml). Also, follicular fluid of smoking women shows a significantly less inhibiting effect on endothelial sVEGFR- 1 production than of non- smoking women (1174.1 ± 93.8 pg/ml compared to 794.2 ± 70.1 pg/ml). Conclusion: A higher concentration of sVEGFR- 1 indicates decreased angiogenic activity in follicles of smokers which may result in reduced blood circulation during follicular growth and thus impaired oocyte maturation by malnutrition.展开更多
目的探讨子痫前期孕妇血清内分泌腺源性血管内皮生长因子(EG-VEGF)、中性粒细胞淋巴细胞比值(NLR)、血小板淋巴细胞比值(PLR)表达与病情严重程度的相关性。方法回顾性分析2021年10月~2023年3月在承德市中心医院妇儿院区产检的90例孕妇...目的探讨子痫前期孕妇血清内分泌腺源性血管内皮生长因子(EG-VEGF)、中性粒细胞淋巴细胞比值(NLR)、血小板淋巴细胞比值(PLR)表达与病情严重程度的相关性。方法回顾性分析2021年10月~2023年3月在承德市中心医院妇儿院区产检的90例孕妇临床资料,根据是否患有子痫前期进行分组,将健康正常的30例孕妇纳入健康对照组,将确诊为子痫前期的60例孕妇纳入观察组,且观察组根据子痫前期严重程度分为轻度组(n=30)和重度组(n=30),比较健康对照组和观察组、轻度组和重度组的血清EG-VEGF、NLR、PLR表达水平,并应用ROC曲线分析血清EG-VEGF、NLR、PLR诊断子痫前期和评价病情严重程度的效能。结果观察组的血清EG-VEGF、NLR高于健康对照组[(53.65±17.46)pg ml vs.(28.93±9.37)pg ml、(3.15±1.02)vs.(2.12±0.67)],PLR低于健康对照组[(98.53±21.86)vs.(115.31±18.36)],差异有统计学意义(t=7.237、5.010、3.613,P<0.05)。经ROC曲线分析发现,血清EG-VEGF、NLR、PLR诊断子痫前期效能良好(P<0.05)。重度组的血清EG-VEGF、NLR高于轻度组[(57.93±13.19)pg ml vs.(49.63±15.63)pg ml、(3.40±0.95)vs.(2.93±0.71)],PLR低于轻度组[(163.72±35.41)vs.(146.70±28.63)],差异有统计学意义(t=2.223、2.171、2.047,P<0.05)。经ROC曲线分析,发现血清EG-VEGF、NLR、PLR评价子痫前期病情严重程度的效能良好(P<0.05)。结论子痫前期孕妇血清EG-VEGF、NLR呈高表达水平,PLR呈低水平表达,且病情越严重的孕妇血清EG-VEGF、NLR越高,PLR越低,且经ROC分析血清EG-VEGF、NLR、PLR在子痫前期和病情严重程度诊断评价方面效能良好。展开更多
文摘Objective: An adequate vascularization in the ovary is important for optimal oocyte maturation. By secreting vascular endothelial growth factor (VEGF) granulosa cells promote blood vessel growth. In addition, they paracrinely reduce the production of VEGF antagonist sVEGFR- 1 (soluble VEGF receptor 1) in endothelial cells. In the present study we examined the influence of smoking on the sVEGFR- 1 concentration in human follicular fluid and on the paracrine activity of granulosa cells. Material and Methods: Follicular fluid samples were obtained during oocyte retrieval from 50 smoking and 50 non- smoking women undergoing in- vitro fertilization treatment. Follicular fluids were used either directly for sVEGFR- 1 quantification or added to endothelial cell cultures to determine the soluble receptor concentration in culture supernatant after four days’ incubation. Results: In this study we were able to show that smokers have a distinctly higher concentration of soluble VEGF receptor 1 in follicular fluid than non- smokers (499.6± 77.7 pg/ml versus 159.2± 29.8 pg/ml). Also, follicular fluid of smoking women shows a significantly less inhibiting effect on endothelial sVEGFR- 1 production than of non- smoking women (1174.1 ± 93.8 pg/ml compared to 794.2 ± 70.1 pg/ml). Conclusion: A higher concentration of sVEGFR- 1 indicates decreased angiogenic activity in follicles of smokers which may result in reduced blood circulation during follicular growth and thus impaired oocyte maturation by malnutrition.
文摘目的探讨子痫前期孕妇血清内分泌腺源性血管内皮生长因子(EG-VEGF)、中性粒细胞淋巴细胞比值(NLR)、血小板淋巴细胞比值(PLR)表达与病情严重程度的相关性。方法回顾性分析2021年10月~2023年3月在承德市中心医院妇儿院区产检的90例孕妇临床资料,根据是否患有子痫前期进行分组,将健康正常的30例孕妇纳入健康对照组,将确诊为子痫前期的60例孕妇纳入观察组,且观察组根据子痫前期严重程度分为轻度组(n=30)和重度组(n=30),比较健康对照组和观察组、轻度组和重度组的血清EG-VEGF、NLR、PLR表达水平,并应用ROC曲线分析血清EG-VEGF、NLR、PLR诊断子痫前期和评价病情严重程度的效能。结果观察组的血清EG-VEGF、NLR高于健康对照组[(53.65±17.46)pg ml vs.(28.93±9.37)pg ml、(3.15±1.02)vs.(2.12±0.67)],PLR低于健康对照组[(98.53±21.86)vs.(115.31±18.36)],差异有统计学意义(t=7.237、5.010、3.613,P<0.05)。经ROC曲线分析发现,血清EG-VEGF、NLR、PLR诊断子痫前期效能良好(P<0.05)。重度组的血清EG-VEGF、NLR高于轻度组[(57.93±13.19)pg ml vs.(49.63±15.63)pg ml、(3.40±0.95)vs.(2.93±0.71)],PLR低于轻度组[(163.72±35.41)vs.(146.70±28.63)],差异有统计学意义(t=2.223、2.171、2.047,P<0.05)。经ROC曲线分析,发现血清EG-VEGF、NLR、PLR评价子痫前期病情严重程度的效能良好(P<0.05)。结论子痫前期孕妇血清EG-VEGF、NLR呈高表达水平,PLR呈低水平表达,且病情越严重的孕妇血清EG-VEGF、NLR越高,PLR越低,且经ROC分析血清EG-VEGF、NLR、PLR在子痫前期和病情严重程度诊断评价方面效能良好。
