The ability to create artificial thick tissues is a major tissue engineering problem, requiring the formation of a suitable vascular supply. In this work we examined the ability of inducing angiogenesis in a bioactive...The ability to create artificial thick tissues is a major tissue engineering problem, requiring the formation of a suitable vascular supply. In this work we examined the ability of inducing angiogenesis in a bioactive hydrogel. GYIGSRG （NH2-Gly-Tyr-Ile- Gly-Ser-Arg-Gly-COOH, GG） has been conjugated to sodium alginate （ALG） to synthesize a biological active biomaterial ALG-GG. The product was characterized by IH NMR, FT-IR and elemental analysis. A series of CaCO3/ALG-GG composite hydrogels were prepared by crosslinking ALG-GG with D-glucono-8-1actone/calcium carbonate （GDL/CaCO3） in different molar ratios. The mechanical strength and swelling ratio of the composite hydrogels were studied. The results revealed that both of them can be regulated under different preparation conditions. Then, CaCO3/ALG-GG composite hydrogel was im- planted in vivo to study the ability to induce angiogenesis. The results demonstrated that ALG-GG composited hydrogel can induce angiogenesis significantly compared with non-modified ALG group, and it may be valuable in the development of thick tissue engineering scaffold.展开更多
基金supported by the National Basic Research Program of China (973 Project,2011CB606202)
文摘The ability to create artificial thick tissues is a major tissue engineering problem, requiring the formation of a suitable vascular supply. In this work we examined the ability of inducing angiogenesis in a bioactive hydrogel. GYIGSRG （NH2-Gly-Tyr-Ile- Gly-Ser-Arg-Gly-COOH, GG） has been conjugated to sodium alginate （ALG） to synthesize a biological active biomaterial ALG-GG. The product was characterized by IH NMR, FT-IR and elemental analysis. A series of CaCO3/ALG-GG composite hydrogels were prepared by crosslinking ALG-GG with D-glucono-8-1actone/calcium carbonate （GDL/CaCO3） in different molar ratios. The mechanical strength and swelling ratio of the composite hydrogels were studied. The results revealed that both of them can be regulated under different preparation conditions. Then, CaCO3/ALG-GG composite hydrogel was im- planted in vivo to study the ability to induce angiogenesis. The results demonstrated that ALG-GG composited hydrogel can induce angiogenesis significantly compared with non-modified ALG group, and it may be valuable in the development of thick tissue engineering scaffold.
