Functional responses to angiotensin Ⅱ (AT-Ⅱ) were determined in aortic vascular smooth muscle cells (VSMCs) from experimental cirrhotic rats. Our data showed that AT-Ⅱ-stimulated extracellular acidification rate (E...Functional responses to angiotensin Ⅱ (AT-Ⅱ) were determined in aortic vascular smooth muscle cells (VSMCs) from experimental cirrhotic rats. Our data showed that AT-Ⅱ-stimulated extracellular acidification rate (ECAR), which was measured by Cytosensor microphysiometry, was significantly reduced in the aortic VSMCs from the cirrhotic rats as compared to those from the control animals. The ability of AT-Ⅱ to promote formation of inositol phosphates, the second messenger produced by the activation of Gq-coupled receptors, was also considerably suppressed in the cirrhotic VSMCs. Furthermore, the maximal p42/44 MAPK phosphorylation stimulated by AT-Ⅱ was significantly reduced in the cirrhotic VSMCs in contrast to that in the normal VSMCs. Taken together, our data clearly demonstrated that the functional responses to AT-Ⅱ was severely suppressed in aortic VSMCs in cirrhosis, indicating the impairment of general Gq-coupled receptor signaling and subsequent biological function in the cirrhotic VSMCs.展开更多
Objective：To observe the clinical efficacy of acupuncture for vascular cognitive disorder following a cerebral infarction.Methods：A total of 90 cases were randomly allocated into an observation group and a control g...Objective：To observe the clinical efficacy of acupuncture for vascular cognitive disorder following a cerebral infarction.Methods：A total of 90 cases were randomly allocated into an observation group and a control group.In addition to the cognitive training and the routine rehabilitation exercise for the paralyzed limb,special needling techniques were employed in the observation group,versus electroacupuncture in the control group.After a session of 4-week treatment,the outcomes were assessed and analyzed usingmini-mental state examination （MMSE）,clock drawing test （CDT）,delayed story recall （DSR） and functional ability questionnaire （FAQ）.Results：There were statistical significances （P0.05） in the scores between before and after treatment in the observation group.Also,there were statistical significances （P0.05） between the observation group and the control group after the treatment.Conclusion：The special needling method in this study could obtain better therapeutic efficacy than electroacupuncture for vascular cognitive disorder.展开更多
Objective：To observe the clinical efficacy of scalp-acupuncture-based therapy for vascular dementia.Methods：Eighty patients with vascular dementia were randomly allocated into 3 groups by the visiting order,27 cases...Objective：To observe the clinical efficacy of scalp-acupuncture-based therapy for vascular dementia.Methods：Eighty patients with vascular dementia were randomly allocated into 3 groups by the visiting order,27 cases in the scalp acupuncture group were treated with needling scalp stimulation areas;25 cases in the body acupuncture group were treated with body acupoints;28 cases in the control group were given Duxil for oral administration.Themini-mental state examination （MMSE） and Barthel index （BI） were used to evaluate the differences between before and after treatments.Results：The total effective rate in the scalp acupuncture group was 92%,versus 89% in the body acupuncture group and 83% in the control group,showing a statistical significance （P0.01）.After the treatments,the MMSE and BI scores showed significant differences in all three groups （P0.01）.After the treatment,the MMSE and BI scores in the scalp acupuncture and body acupuncture groups showed statistical significances compared with the control group （P0.05）.There was also a statistical significance between the scalp acupuncture group and body acupuncture group （P0.05）.Conclusion：Scalp-acupuncture-based therapy can substantially improve MMSE and BI scores and thus obtain a more remarkable effect for vascular dementia.展开更多
文摘Functional responses to angiotensin Ⅱ (AT-Ⅱ) were determined in aortic vascular smooth muscle cells (VSMCs) from experimental cirrhotic rats. Our data showed that AT-Ⅱ-stimulated extracellular acidification rate (ECAR), which was measured by Cytosensor microphysiometry, was significantly reduced in the aortic VSMCs from the cirrhotic rats as compared to those from the control animals. The ability of AT-Ⅱ to promote formation of inositol phosphates, the second messenger produced by the activation of Gq-coupled receptors, was also considerably suppressed in the cirrhotic VSMCs. Furthermore, the maximal p42/44 MAPK phosphorylation stimulated by AT-Ⅱ was significantly reduced in the cirrhotic VSMCs in contrast to that in the normal VSMCs. Taken together, our data clearly demonstrated that the functional responses to AT-Ⅱ was severely suppressed in aortic VSMCs in cirrhosis, indicating the impairment of general Gq-coupled receptor signaling and subsequent biological function in the cirrhotic VSMCs.
文摘Objective：To observe the clinical efficacy of acupuncture for vascular cognitive disorder following a cerebral infarction.Methods：A total of 90 cases were randomly allocated into an observation group and a control group.In addition to the cognitive training and the routine rehabilitation exercise for the paralyzed limb,special needling techniques were employed in the observation group,versus electroacupuncture in the control group.After a session of 4-week treatment,the outcomes were assessed and analyzed usingmini-mental state examination （MMSE）,clock drawing test （CDT）,delayed story recall （DSR） and functional ability questionnaire （FAQ）.Results：There were statistical significances （P0.05） in the scores between before and after treatment in the observation group.Also,there were statistical significances （P0.05） between the observation group and the control group after the treatment.Conclusion：The special needling method in this study could obtain better therapeutic efficacy than electroacupuncture for vascular cognitive disorder.
基金supported by Shaanxi Provincial Science & Technology Research Development Project [2005K16-G6(5)]
文摘Objective：To observe the clinical efficacy of scalp-acupuncture-based therapy for vascular dementia.Methods：Eighty patients with vascular dementia were randomly allocated into 3 groups by the visiting order,27 cases in the scalp acupuncture group were treated with needling scalp stimulation areas;25 cases in the body acupuncture group were treated with body acupoints;28 cases in the control group were given Duxil for oral administration.Themini-mental state examination （MMSE） and Barthel index （BI） were used to evaluate the differences between before and after treatments.Results：The total effective rate in the scalp acupuncture group was 92%,versus 89% in the body acupuncture group and 83% in the control group,showing a statistical significance （P0.01）.After the treatments,the MMSE and BI scores showed significant differences in all three groups （P0.01）.After the treatment,the MMSE and BI scores in the scalp acupuncture and body acupuncture groups showed statistical significances compared with the control group （P0.05）.There was also a statistical significance between the scalp acupuncture group and body acupuncture group （P0.05）.Conclusion：Scalp-acupuncture-based therapy can substantially improve MMSE and BI scores and thus obtain a more remarkable effect for vascular dementia.
