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lncRNA ANCR调控miR-497-5p对胃癌细胞血管管腔形成及凋亡的影响
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作者 朱婷婷 夏一函 +2 位作者 孙莎莎 高鹏 邱文生 《中国临床研究》 CAS 2024年第2期216-222,共7页
目的探究长链非编码RNA(lncRNA)ANCR调控miR-497-5p对胃癌细胞血管管腔形成、凋亡及PI3K/AKT信号通路的影响。方法取MGC803胃癌细胞将其分为胃癌细胞(GC)组、胃癌细胞+lncRNA ANCR-NC(AN)组、胃癌细胞+lncRNA ANCR过表达质粒(AM)组、胃... 目的探究长链非编码RNA(lncRNA)ANCR调控miR-497-5p对胃癌细胞血管管腔形成、凋亡及PI3K/AKT信号通路的影响。方法取MGC803胃癌细胞将其分为胃癌细胞(GC)组、胃癌细胞+lncRNA ANCR-NC(AN)组、胃癌细胞+lncRNA ANCR过表达质粒(AM)组、胃癌细胞+lncRNA ANCR siRNA(AI)组、胃癌细胞+miR-497-5p-NC(MN)组、胃癌细胞+miR-497-5p siRNA(MI)组、胃癌细胞+miR-497-5p mimics(MM)组、胃癌细胞+lncRNA ANCR siRNA+miR-497-5p mimics(IM)组,管腔形成实验观察细胞血管管腔形成,PI染色法检测细胞凋亡,免疫印迹法检测PI3K和AKT蛋白表达,双荧光素酶报告实验验证lncRNA ANCR和miR-497-5p的相互作用。结果与AN组、MN组相比,AM组、MI组凋亡率明显降低(P<0.05),血管管腔形成数量、PI3K和AKT蛋白表达明显升高(P<0.05);与AM组、MI组相比,AI组、MM组凋亡率明显升高(P<0.05),血管管腔形成数量、PI3K和AKT蛋白表达明显降低(P<0.05);与AI组、MM组相比,IM组凋亡率明显升高(P<0.05),血管管腔形成数量、PI3K和AKT蛋白表达明显降低(P<0.05)。双荧光素酶报告结果显示,转染lncRNA ANCR后可显著降低miR-497-5p-3′-UTR-WT的荧光素酶活性(P<0.05),但对突变基因无影响(P>0.05)。结论抑制lncRNA ANCR可有效抑制胃癌细胞血管管腔形成,促进细胞凋亡,并抑制PI3K和AKT信号通路表达,其作用机制可能与靶向激活miR-497-5p有关。 展开更多
关键词 胃癌 非编码RNA ANCR miR-497-5p 血管管腔形成 凋亡 PI3K/AKT信号通路
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血管管腔形成的遗传调控
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作者 曾健 兰雨 杨晓 《生命科学》 CSCD 2015年第3期262-267,共6页
血管系统是脊椎动物机体中最早发育并行使重要生理功能的复杂系统。血管管腔形成启始于心脏开始跳动和所有其他组织器官形成之前,对于促进血管系统的精确建成和有效灌流,通过营养运输、气体交换和代谢废物清除以确保所有组织器官的形成... 血管系统是脊椎动物机体中最早发育并行使重要生理功能的复杂系统。血管管腔形成启始于心脏开始跳动和所有其他组织器官形成之前,对于促进血管系统的精确建成和有效灌流,通过营养运输、气体交换和代谢废物清除以确保所有组织器官的形成和生长至关重要。在血管发育过程的两个阶段,即血管发生(vasculogenesis)和血管形成(angiogenesis)中,有效管腔的形成和维持均是保证血管正常发育并行使生理功能的关键环节。血管管腔形成大致可以分为两个关键环节:血管管腔形成的诱导以及内皮细胞极性的建立和维持。重点依据体内遗传修饰模式生物方面的研究结果,从上述两个环节分别阐述血管管腔形成的遗传调控机制。 展开更多
关键词 血管管腔形成 细胞极性 细胞骨架重排
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COMBINED INTRAOPERATIVE ILIAC ARTERY STENTS AND FEMORO- POPLITEAL BYPASS FOR MULTILEVEL ATHEROSCLEROTIC OCCLUSIVE DISEASE
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作者 刘昌伟 管珩 +2 位作者 李拥军 郑曰宏 刘卫 《Chinese Medical Sciences Journal》 CAS CSCD 2001年第3期165-168,共4页
Objective. To review our preliminary experience and evaluate our early results of a combined intraoperative iliac angioplasty and stenting with infrainguinal revascularization in multilevel atherosclerotic occlusive d... Objective. To review our preliminary experience and evaluate our early results of a combined intraoperative iliac angioplasty and stenting with infrainguinal revascularization in multilevel atherosclerotic occlusive disease. Methods. From July 1999 to April 2000, intraoperative iliac angioplasty and stenting combined with simultaneous femoro- popliteal bypass were performed on 12 lower extremities of 10 patients suffering from multilevel atherosclerotic occlusive disease. There were 8 men and 2 women, average 72 years. The indications for procedures included disabling claudication in 3 and rest pain in 7 patients. Results. Eleven iliac angioplasty and stent procedures combined with simultaneous 9 femoro- popliteal bypass and 3 femoro- femoral- popliteal bypass were performed in 12 limbs of 10 patients. Angioplasty and stent placement was technically successful in all patients. One contralateral femoral- popliteal bypass was failure after femorofemoral- popliteal bypass. There were no additional instances of procedural or postoperative morbidity or mortali- ty. Mean follow- up was 5 months (range 1~ 10 months). During the follow- up period, one femoro- infrapopliteal graft became occluded after 7 months and above- knee amputation was required. The cumulative primary patency rate of stented iliac arteries, femoro- femoral bypass grafts and femoro- popliteal bypass grafts were 100% (11/11), 100% (3/3) and 90.9% (10/11) in the follow- up period, respectively. The amputation rate was 8.3% (1/12). Conclusions. Intraoperative iliac artery PTA and stent placement can be safely and effectively performed simultaneously with infrainguinal revascularization for multilevel atherosclerotic occlusive disease by skilled vascular surgeon, using a portable C arm fluoroscopy in the operating room. Furthermore, iliac artery PTA and stenting was valuable adjunct to distal bypass either to improve inflow and outflow, or to reduce the extent of traditional surgical intervention, and also, any angioplasty and stenting- related complications can be immediately corrected as well. 展开更多
关键词 atherosclerotic occlusive disease iliac angioplasty STENTING femoro- popliteal bypass
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