血细胞浓度对猪血豆腐品质的影响

为探明血细胞浓度对猪血豆腐品质的影响,以生理盐水替代部分血细胞的方法调整原血中血细胞浓度,并分析不同血细胞浓度的猪血制成的猪血豆腐样品的质构、感官、色泽和持水性品质。结果表明:随着血细胞浓度的降低,猪血豆腐的硬度显著降低,破裂力和剪切力先增大后减小,红度a*值和黄度b*值随血细胞浓度减小而降低,持水性随血细胞浓度降低先减小后增大。降低猪血中血细胞浓度能够显著改善猪血豆腐的口感品质,血细胞浓度在0.68×10^(9)~2.80×10^(9)个/mL时,血豆腐的总体接受度最高。

皮层下动脉硬化性脑病患者外周血红细胞和血小板内钙离子含量的变化

研究Binswager病患者外周血血细胞内钙离子浓度 ,探讨钙离子异常分布与Binswager病形成的关系 ,并寻找预防措施。Binswager病患者 2 6例 ,健康对照者 2 0例 ,采用流式细胞术检测外周血血细胞内钙离子浓度。结果发现 ,Binswager病组血细胞内钙离子浓度显著高于对照组 (P <0 .0 5 )。重度脑萎缩组钙离子含量高于轻中度脑萎缩组。智能检查评分结果发现 ,痴呆组血细胞钙离子增加较正常组及痴呆前期组明显 (P <0 .0 5 )。结果提示 ,Binswager病形成机制与钙离子浓度分布异常有关。钙离子分布异常可能激活细胞内源性核酶和T淋巴细胞受体的交联诱发突触传递中神经递质释放 ,使红细胞变形能力和血小板聚集性改变 ,加重脑组织缺血、缺氧程度。应用钙拮抗剂可预防因脑循环障碍所致的Binswager病。

Clinical evaluation of serum concentrationsof intercellular adhesion molecule-1 in patients with colorectal cancer

AIM： To investigate the correlation between the serum soluble intercellular adhesion molecule-1 （sICAM-1） and the clinicopathologic features and to evaluate the possible prognostic significance of sICAM-1 concentration in colorectal cancer.METHODS： A total of 56 patients （mean age 57.3 years） having transitional cell carcinoma of the colorectal and 25 control patients （mean age 42.6 years） were enrolled in the study. The serum samples of the patients were obtained on the day before surgery. Sera were obtained by centrifugation, and stored at -80℃ until assay. Serum concentrations of ICAM-1 were measured with enzymelinked immunoassay. Differences between the two groups were analyzed by Student＇s t-test.RESULTS： No significant increase of serum slCAM-1 could be demonstrated in the Dukes A1 patients （352.63±61.82μg/L） compared to the control group （345.72±49.81 μg/L,/〉〉0.05）, Dukes A1 patients （352.63±61.82 μg/L）compared to Dukes A2,3 patients （491.17±86.36μg/L,P〈0.05）. Furthermore, the patients with Dukes B had significantly higher serum concentrations of sICAM-1 than those of the control group （496.82±93.04μg/L vs 345.72±49.81μg/L, P〈0.01）. Compared with Dukes A2,3,B colorectal cancer patients, patients with more advanced clinical stage （Dukes C and D） had higher levels of sICAM-1 （743.68±113.74 μg/L vs491.17±86.36μg/L and 496.82±93.04 μg/L, P〈0.001）. The difference was statistically significant in sICAM-1 levels between patients with positive lymph node status and those without lymph node involvement （756.25±125.57μg/L vs 445.62±69.18 μg/L, P〈0.001）.Patients with poorly differentiated colorectal cancer had a higher level of slCAIVl-1 than those with differentiated and highly differentiated cancer （736.49±121.97μg/L vs410.23±67.47 μg/L, P〈0.001）.CONCLUSION： In this study, serum ICAM-1 levels were found to be related to tumor presence, clinical stages,and grade. Increased ICAM-1 in patients with colorectal cancer which should be considered when the diagnostic and/or prognostic usefulness of soluble ICAM-1 is to be evaluated, sICAM-1 should prove useful for monitoring malignant disease stage and for evaluating the effectiveness of various therapeutic approaches for colorectal carcinomas.

Early effects of Lansoprazole orally disintegrating tablets on intragastric pH in CYP2C19 extensive metabolizers

AIM: To compare rabeprazole (RPZ; 10 mg) with Lansoprazole orally disintegrating tablets (LPZ; 30 mg OD) in terms of antisecretory activity and blood drug concentration after a single dose. METHODS: Eight H pylori-negative cytochrome P450 (CYP) 2C19 extensive metabolizers were assigned to receive a single oral dose of RPZ 10 mg or LPZ 30 mg OD. Twelve hour intragastric pH monitoring was perform- ed on the day of treatment. Blood samples were also collected after the administration of each drug. RESULTS: LPZ 30 mg OD induced a significantly earlier rise in blood drug concentration than RPZ 10 mg; consequently, LPZ 30 mg OD induced a significantly earlier rise in median pH in the third and fourth hours of the study. CONCLUSION: In H pylori-negative CYP2C19 extensive metabolizers, LPZ 30 mg OD induced a significantly faster inhibition of gastric acid secretion than RPZ 10 mg.

5-Fluorouracil concentration in blood, liver and tumor tissues and apoptosis of tumor cells after preoperative oral 5'-deoxy-5-fluorouridine in patients with hepatocellular carcinoma

AIM: To study the levels of 5-fluorouracail (5-FU) in plasma, liver and tumor in patients with hepatoceilular carcinoma after oral administration of 5'-deoxy-5-fluorouridine (5'-DFUR). METHODS: Thirty-nine patients with hepatoceilular carcinoma were treated with oral 5'-DFUR for more than 4 d before operation. The contents of 5-FU in plasma, liver and tumor were measured by high performance liquid chromatography (HPLC) and apoptosis of tumor cells was evaluated by in-situ TUNEL after resection of tumor. RESULTS: The concentrations of 5-FU were 1.1 μg/mL, 5.6, 5.9, and 10.5 μg/g in plasma, the liver tissue, the center of tumor and the periphery of tumor, respectively. 5-FU concentration was significantly higher in the periphery of tumor than that in the liver tissue and the center of tumor (10.5±1.6 μg/g vs 5.6±0.8 μg/g, t = 21.38, P<0.05; 10.5±1.6 μg/g vs 5.9±0.9 μg/g, t = 20.07, P<0.05). 5-FU level was significantly lower in plasma than that in the liver and the tumor (1.1±0.3 μg/mL vs 5.6±0.8 μg/g, t = 19.63, P<0.05; 1.1±0.3 μg/mL vs 10.5±1.6 μg/g, t= 41.01, P<0.05). Apoptosis of tumor cells was significantly increased after oral 5'-DFUR compared to the control group without 5-DFUR treatment. CONCLUSION: There is a higher concentration of 5-FU distributed in the tumor compared with liver tissue and apoptosis of tumor cells is increased following oral 5'-DFUR compared with the control group. The results indicate that 5'-DFUR is hopeful as neo-adjuvant chemotherapy to prevent recurrence after resection of hepatoceilular carcinoma.

妊娠期贫血患者的血常规检验价值

目的探讨妊娠期贫血患者实施血常规检验的价值。方法纳入2016年12月至2018年2月本院收治的妊娠期贫血患者60例作为研究组,同期选择60例健康体检孕妇作为对照组进行研究,两组受检者均实施血常规检验(MCH-红细胞平均血红蛋白含量;RDW-血细胞分布宽度;Hb-血红蛋白;RBC-红细胞;MCV-红细胞平均体积;MCHC-血细胞平均血红蛋白浓度),对比两组检验结果及研究组贫血情况。结果检验结果对比:对照组优于研究组,其中对照组MCH、RDW低于研究组,Hb、RBC、MCV、MCHC高于对照组,P<0.05。贫血情况对比:孕早期、孕中期小细胞贫血率高于孕晚期,大细胞贫血率低于孕晚期。结论妊娠期贫血患者实施血常规检验的价值显著,孕期女性体内纤溶、抗凝系统异常,明显增加贫血发生率,借助血常规检验临床医师可准确掌握孕妇具体贫血情况,为临床医师诊断提供较高参考价值,并实施针对性治疗、干预,降低对胎儿的影响,最大限度确保胎儿在母体内健康生长、发育,改善母婴分娩结局,值得借鉴。

Therapeutic Efficacy of Quinapyramine Sulphate with Freund＇s Complete Adjuvant in Wistar Rats Infected with Trypanosoma Congolense

Therapeutic efficacy of QS （quinapyramine sulphate） and FCA （Freund＇s complete adjuvant） combination was studied. The aim of the study was to evaluate therapeutic efficacy of QS using FCA in Trypanosorna congolense infection. GrouPs treated with QS and FCA had parasite disappeared in peripheral circulation 2 days pi, relapse was observed one week later. Effect of treatment on rectal temperature shows no significance （p 〈 0.05）, normalization of rectal temperature occurred in QS and FCA treated groups （34.1℃） than untreated （42.8 ℃）, QS （37.4 ℃） and FCA （35.92 ℃） treated groups. Mean body weight was significant （p 〈 0.001） in QS and FCA, QS, and FCA groups. Packed cell volume and hemoglobin concentration for untreated groups were lower, but increased in QS, FCA, QS and FCA treated groups, indicating anemia amelioration. White blood cell counted in untreated, QS and FCA treated groups showed no significance （p 〈 0.05）, however, there was leukocytosis due to lymphocytosis in QS and FCA treated group （6.79 × 10^3/μl） compared with untreated and other groups. There was comparative decrease in serum liver enzymes in QS and FCA treated group than other groups. Therefore, QS at lower recommended dose with FCA may enhance efficacy of QS in trypanosomiasis.

Wortmannin induced apoptosis of leukemia cells by reducing PI3K/Akt

Objective： We studied the effects of Wortmannin （WM） on the proliferation and apoptosis of leukemia cells, and explore the possible mechanisms. Methods： The human myeloid leukemia cell line K562 was treated with different concentrations of WM, and then detected the activity of the cell proliferation by MTT assay, comet tail formation of cell DNA damage phenomenon by single cell gel electrophoresis, cell apoptosis byAnnexin V-FITC/PI double staining and the expression levels of total Akt, phoshorylated Akt, NF-KB and protein in K562 cell by Western blotting, RT-PCR test before and after WM. Results： WM inhibited cell proliferation of K562 in a concentration-dependent manner, with IC50 value for 24 h being 25 nmol/L. WM induced apoptosis of K562 cells in a concentration-dependent manner, and could induce the breakage of DNA strand of K562 cell. The rate of DNA tail and the tail length of experimental groups were significantly higher than that of control group. WM may inhibit the expression of phosphorylated Akt and NF-KB protein in a dose-dependent manner in both the protein and gene levels, but no significant effect on total Akt protein. Conclusion： WM inhibited cell proliferation and induced apoptosis in K562 and concentration-dependent manner. The possible mechanism may be involved in the regulation of survival signaling pathway, such as PI3K/Akt/NK-KB.

In vitro and in vivo studies on as-extruded Mg-5.25wt.%Zn-0.6wt.%Ca alloy as biodegradable metal

Magnesium alloys have shown prospective applications as a new biodegradable metal within bone. To garantee the longterm biocompatibility, a Mg-Zn-Ca alloy,composing of essential elements for human, was prepared and its feasibility for orthopedic applications was investigated. The in vitro and in vivo corrosion of Mg-Zn-Ca alloy as well as the biocompatibility were studied. The in vitro corrosion tests in five kinds of physiological solutions showed that the corrosion rates and corrosion morphologies of the alloy were strongly influenced by the solution used. The addition of serum in Hank’s and MEM significantly slowed down the corrosion rate and improved the corrosion uniformity of the alloy. The corrosion rate decreased with increasing serum concentration.The alloy showed the slowest corrosion rate as well as homogeneous corrosion morphology in MEM＋10%FBS. Both the indirect and direct cell experiments indicated good cytocompatibility of the extruded Mg-Zn-Ca alloy. In vivo, we observed a gradual degradation process from the surface of extruded Mg-Zn-Ca alloy and only 40% in volume of implant was left after 4 weeks implantation in medullary cavities of mice. The micro-CT and histological analyses revealed its good biocompatibility with peri-implant new bone formation and increasing cortical bone thickness with increasing implantation period. This study showed that the extruded MgZn-Ca alloy provided sufficient biocompatibility for orthopedic application, though the in vivo corrosion rate should be further reduced for clinical use.

Decreased serum prohepcidin concentration in patients with polycythemia vera

Objective： Iron deficiency is a common complication in patients with polycythemia vera （PV）. Hepcidin is a principal regulator of iron homeostasis. The aim of our study was to assess prohepcidin, a hepcidin precursor, and other iron status pa- rameters in the serum of PV patients. Methods： The study was performed in 60 patients （F/M 26/34） aged 38-84 （66＋ 10） years. The control group consisted of 20 healthy volunteers, age and sex matched. The following parameters were determined in blood serum samples： prohepcidin concentration, iron content, unsaturated iron binding capacity （UIBC）, total iron binding capacity （TIBC）, transferrin saturation （TfS）, and concentrations of ferritin and soluble transferrin receptor （sTfR）. Results： All PV patients showed significantly lower levels of prohepcidin, higher levels of sTIR and TIBC compared to the control group. 40% of the patients from the study group showed concentrations of ferritin below the normal range and significantly lower levels of serum iron and TfS, and significantly higher levels of sTIR, UIBC and TIBC in comparison with the rest of the study group. In this group of patients, prohepcidin concentrations were significantly lower than those in other patients. Conclusion： The results indicate that PV patients suffer from iron metabolism disorders. The decreased serum level ofprohepcidin in PV patients may be a result of iron deficiency.

Pulsatile blood flow through stenosed artery with axial translation

The study of pulsatile blood flow through axisymmetric stenosed artery subject to an axial translation has been attempted with hematocrit concentration-dependent blood viscosity. The heart contraction and subsequent relaxation generate periodic pressure gradient in blood flow and translation in the artery can be represented by Fourier series. Numerical data required for computing Fourier harmonics for the pressure gradient and acceleration in the artery has been simulated from pressure waveform graph and biplanar angiogram. Velocity field has been obtained by solving governing equation using variational Ritz method. The hemodynamic indicators WSS, AWSS, OSI, RRT are derived and computed numerically. The effects of thickness of stenosis, and hematocrit concentration index on these indicators are computed and analyzed through graphs.