基于SNP芯片对里岔黑猪进行遗传多样性与遗传结构分析的研究

本实验旨在利用基因芯片对里岔黑猪进行保种分析和群体结构分析,理清该品种的遗传结构和血统关系,以更好地保护和利用里岔黑猪这一重要的地方猪种质资源。实验利用“中芯一号”基因芯片对里岔黑猪省级保种场的109头能繁公母猪进行SNP测定,进而实施群体遗传多样性、遗传结构、近交程度和家系分析。结果显示,里岔黑猪的有效群体含量为5.88;通过主成分分析、G矩阵和聚类分析,里岔黑猪整个群体分为8个公猪家系和1个非公猪家系(28头母猪不属于这8个公猪家系)。在109头里岔黑猪中共检测到4250个基因组上的长纯合片段(Runs of Homozygosity, ROH),ROH长度在300~450 Mb之间的个体数目最多,占39.45%,平均ROH长度为343.56(±18.251)Mb;ROH数量40~50个的个体最多,占34.86%,平均为38.99(±1.461)个;将每个个体ROH数据汇总后计算出该群体的近交系数平均值为0.14。研究表明,胶州市茂华里岔黑猪良种场的群体在长期选育过程中遗传多样性丰富,但群体中近交现象明显,为确保其优良性状能够长期延续,可结合芯片数据,通过轮回杂交的方式,减缓群体近交系数的增长,或者从其他里岔黑猪保种场引入公猪,进行血统更新。

新疆褐牛血缘分析和育种对策研究

新疆褐牛育种历史长达70多年,是我区畜牧工作者经过了几代人的辛勤努力选育成的一个乳肉兼用型品种。它是由阿拉托乌牛和少量科斯特罗姆牛,以及瑞士褐牛与我区的哈萨克牛进行杂交而育成。是我区养牛业的主要品种,自1983年品种建立以来,没有制定系统的育种工作,造成品种退化,因此,研究血统、确定育种方向十分重要。

供应链运营数据治理体系研究与应用

随着国网陕西省电力有限公司供应链运营工作深入开展,供应链感知能力不断增强,供应链全过程海量数据全面汇聚。为了持续提升供应链运营质效,公司成立了数据治理专项团队,建立数据治理各项机制,制定数据治理流程规范,坚持问题闭环管理,通过“专项 + 常态”模式,稳步提升数据质量。

Effects of yiqi yangyin huoxue therapy on IgA nephropathy: a systematic review and meta-analysis of randomized controlled trials

Objective To assess the efficacy and safety of Yiqi Yangyin Huoxue therapy (YYHT) for the treatment of IgA nephropathy. Methods Databases articles about YYHT in treating IgAN patients were searched through February 2018. Comparisons were YYHT alone or YYHT in combination with western medicine as experimental intervention, and western medicine as the control. Randomized controlled trials (RCTs) were selected. Cochrane collaboration tool was used for assessing risk of bias to evaluate methodologies. RevMan 5.3 software was applied to analyze the data of included trials. Results A total of 21 studies involving 1473 patients were included. Meta-analysis results demonstrated that YYHT alone or YYHT in combination with western medicine were more efficacious than only western medicine in decreasing proteinuria [MD =-0.41, 95% CI (-0.53,-0.29), P < 0.00001] and the urine red blood cells [MD =-7.46, 95% CI (-9.28,-5.64), P < 0.00001]. There also showed improvement in the clinical total effective rate [OR = 2.78, 95% CI (2.14, 3.61), P < 0.00001] between the two groups. Some of the included trials did not report the adverse events. Conclusions Current evidence shows that YYHT can reduce proteinuria and urine red blood cells and improve the total efficacy rate. Since poor quality and publication bias of some included trials, more evidence-based medicine studies needed to define the role of YYHT in the treatment of IgAN.

Assessment of Heart Rate Variability and Heart Rate Turbulence Parameters in the Patients with Arterial Hypertension of the II Degree

Background： AH （Arterial hypertension） due to the extremely high incidence is the most important risk factor for development of myocardial infarctions, strokes and lethal outcomes. It is very important to identify a group of patients with increased risk of adverse outcomes to modify treatment and prevention. Significant assistance in resolving this problem is to provide a method of HM （Holter monitoring） which allows estimating the HRV （heart rate variability） and HRT （heart rate turbulence） parameters that are independent risk predictors of sudden cardiac death. The aim of our study was to assess HRV and HRT parameters in patients with AH of the II degree. Materials and methods： We have studied parameters of HRV and HRT in 214 patients with AH of the II-nd degree aged 35 to 70 （57.7 ± 7.6） years： 121 women （56.5%） and 93 men （43.5%）. All patients underwent electrocardiography and echocardiography. Statistical analysis of the results has been made using the analytical package Statistics 10.0. Results： The parameters of HRV and HRT in patients with AH of the II degree were studied. Statistically significant correlations of the HRV parameters with the age of patients, average HR, circadian index, and EF （ejection fraction） were revealed. Statistically significant correlations of the HRT parameters with the LVMI （left ventricular mass index）, EF, HR average were received. High level of the statistical significance of the interrelation of HRT parameters （TO and TS） has been revealed. Conclusions： The received results can be used during the selection of the group of patients with AH having high risk for unfavorable outcomes. Taking into account the features of the HRV and turbulence parameters and factors associated with them this will enable the individualization of the assessment of the risk in AH as much as possible and prescription of adequate treatment for each patient.

Epidemiology and Mutational Analysis of Global Strains of Crimean-Congo Haemorrhagic Fever Virus

Crimean-Congo hemorrhagic fever (CCHF) is a severe illness with high fatality.Cases are reported in several countries in Africa,Europe,the Middle East,and Asia.Phylogenetic analyses based on the virus S (nucleocapsid),M (glycoprotein),and L (polymerase) genome segments sequences indicate distinct geographic lineages exist but their specific genetic characteristics require elucidation.In this work we collected all full length S segment sequences and generated a phylogenetic tree based on the alignment of these 62 samples.We then analyzed the alignment using entries from AAIndex,the Amino Acid Index database,to identify amino acid mutations that performed significant changes in charge,pka,hydropathy and side chain volume.Finally,we mapped these changes back to the tree and alignment to identify correlated mutations or sites that characterized a specific lineage.Based on this analysis we are able to propose a number of sites that appear to be important for virus function and which would be good candidates for experimental mutational analysis studies.

Meta-analysis on protective effect of electroacupuncture at “Nèiguān”(内关 PC 6) in rats with reperfusion injury induced myocardial ischemia

Objective To systematically evaluate the protection effect of electroacupuncture at ＂Neiguan＂（内关 PC 6） on myocardial damage by means of evidence-based medicine.Methods Databases were retrieved by computer,while dissertation compilations and conference proceedings were searched manually.Data were analyzed statistically by using RevMan5.2software.Results Twelve studies were enrolled.Compared with myocardial ischemia reperfusion injury model group,① the magnitude of ECG STⅡ segment elevation can be reduced by means of electroacupuncture at PC 6（WMD=-73.95,95%CI[-109.69,-38.20]） and the difference was statistically significant（P0.0001）;② the ATPase in myocardial cells can be increased by means of electroacupuncture at PC 6（SMD=2.28,95%Cl[1.50,3.07]） and the difference was statistically significant（P0.00001）;③ the release of cTnT can be reduced by means of electroacupuncture at PC 6（WMD=-2.69,95%CI[-3.16,-2.22]） and the difference was statistically significant（P0.00001）;④ the size of myocardial infarction can be reduced by means of electroacupuncture at PC 6（WMD=-17.99,95%Cl[-19.88,-16.09]） and the difference was statistically significant（P0.00001）;⑤ the release of CK can be reduced by means of electroacupuncture at PC 6（WMD=-0.44,95%CI[-0.61,-0.27]） and the difference was statistically significant（P0.00001）;⑥ the release of CK-MB can be reduced by means of electroacupuncture at PC 6（SMD=-2.85,95%CI[-4.46,-1.24]） and the difference was statistically significant（P0.001）;⑦ the release of LDH can be reduced by means of electroacupuncture at PC 6（SMD=-2.97,95%CI[-4.87,-1.07]） and the difference was statistically significant（P0.01）.Conclusion Based on the current experimental evidences,electroacupuncture at PC 6 has a higher protective effect on myocardium.