Objective: Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods: Healthy rabbits were ran...Objective: Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods: Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was per- formed with normal saline (group B), vitamin C (group C) and vitamin E (group D). Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline (sample B), vitamin C (sample C) and vitamin E (sample D). Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results: In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion: Vitamin C can protect vascular endothelial cells from mannitol-induced injury.展开更多
The aim of this study was to analyze the point mutation of the exon 1 at codon 54 of the mannose (or mannan)-binding lectin (MBL) gene in healthy individuals of Chinese Hans and Mongolian population, and to find out a...The aim of this study was to analyze the point mutation of the exon 1 at codon 54 of the mannose (or mannan)-binding lectin (MBL) gene in healthy individuals of Chinese Hans and Mongolian population, and to find out any association between the plasma levels of MBL and the gene mutation frequency in both groups of individuals. Blood samples were collected randomly from 56 healthy individuals of Chinese Hans and 37 Mongolian. The detection of the point mutations of the MBL gene was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and detections for plasma levels of MBL were determined by using MBL ELISA kits. A MBL PCR method of assay was established with high specificity, and good reproducibility. By optimizing the PCR condition, the optimal annealing temperature was 55℃, and the lowest detection limit was 160 pg. No bands were found in non-specificity samples (HAV, HBV, HCV and TB), and the sequences of PCR products were the same as the expected ones. Also a MBL PCR-RFLP was established. Upon electrophoresis of the digested products in 3% agarose gel, there were 3 patterns: in which 2 bands correspond to molecule weight 232 bp and 93 bp; 1 band, corresponds to molecule weight 325 bp and 3 bands correspond to molecule weight 325 bp, 232 bp and 93 bp, respectively. Three bands of 325 bp, 232 bp and 93 bp of point mutations were found at codon 54 of MBL coding gene. Frequencies in healthy Han and Mongolian population were 0.2321 and 0.1757 respectively. The average plasma MBL concentration was 1998.750 μg/L, with SD of 1505.152 in 56 healthy Han population and 2525.676 μg/L, with SD of 1955.188 in 37 Mongolian. A negative correlation between MBL concentration and gene mutation frequency was found in healthy Han population. Frequency of point mutation was 1.00 when the MBL concentrations were below 100 μg/L; frequency of point mutation was 0.4524 when the concentration was 100 μg/L to 1000 μg/L; and the frequency of point mutation was 0.0156 when the concentration was over 1000 μg/L. Analysis of association between MBL concentration and gene frequency in healthy Mongolian population showed that frequency of point mutation was 1.00 when the MBL concentrations were below 100 μg/L and the frequency of point mutation was 0.4583 when the MBL concentrations were 100 μg/L to 1000 μg/L; no point mutation was found when the concentration was over 1000 μg/L. It is concluded that the frequencies of mutation at codon 54 of MBL coding gene had been determined in both healthy Hans and Mongolian population, and the frequency was higher in healthy Hans than that of Mongolian, but no statistical significance (χ 2=0.8574, P >0.05). The MBL level was lower in healthy Hans than in Mongolian population, but there was no statistical significance( t =1.448, 0.1< P <0.2). There was a negative correlation between frequency of point mutation and MBL concentrations in both Hans and Mongolian population( r =-0.62, r =-0.641).展开更多
Reactive oxygen species （ROS） scavengers, including ascorbate peroxidase, superoxide dismutase, catalase and peroxidase, are the most commonly used biomarkers in assessing an organisms＇ response to many biotic and ...Reactive oxygen species （ROS） scavengers, including ascorbate peroxidase, superoxide dismutase, catalase and peroxidase, are the most commonly used biomarkers in assessing an organisms＇ response to many biotic and abiotic stresses. In this study, we cloned an 866 bp GST（phi） gene in Lemna minor and investigated its characteristics, expression and enzymatic activities under 75 lamol/L cadmium concentrations in comparison with other ROS scavengers. GST（phi） gene expression patterns were similar to those of other scavengers of ROS. This suggests that GST（phi） might be involved in responding to heavy metal （cadmium） stress and that its expression level could be used as a bio-indicator in monitoring cadmium pollution.展开更多
Background:White smoke bomb exposure in the military setting could result in organ injuries,which is uncommon and has been rarely described in previous studies.The aim of this study is to observe whether serum zinc le...Background:White smoke bomb exposure in the military setting could result in organ injuries,which is uncommon and has been rarely described in previous studies.The aim of this study is to observe whether serum zinc levels are associated with liver function after white smoke inhalation.Methods:Fifteen patients with white smoke exposure were the subjects in this study.The clinical manifestations,liver function tests and the serum zinc levels were analysed.Results:The level of serum zinc was increased in the moderate or severe group(n=4)compared with that in the mild group(n=11).The four cases in the moderate or severe group had delayed impairment of liver function with an elevation of alanine transaminase.Additionally,increased blood concentrations of hyaluronic acid were found in three patients in the moderate or severe group.But no hexachloroethane or trichloropropane was detected.Conclusion:This study suggested that serum zinc levels may be associated with the severity of liver injuries after white smoke inhalation.展开更多
文摘Objective: Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods: Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was per- formed with normal saline (group B), vitamin C (group C) and vitamin E (group D). Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline (sample B), vitamin C (sample C) and vitamin E (sample D). Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results: In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion: Vitamin C can protect vascular endothelial cells from mannitol-induced injury.
文摘The aim of this study was to analyze the point mutation of the exon 1 at codon 54 of the mannose (or mannan)-binding lectin (MBL) gene in healthy individuals of Chinese Hans and Mongolian population, and to find out any association between the plasma levels of MBL and the gene mutation frequency in both groups of individuals. Blood samples were collected randomly from 56 healthy individuals of Chinese Hans and 37 Mongolian. The detection of the point mutations of the MBL gene was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and detections for plasma levels of MBL were determined by using MBL ELISA kits. A MBL PCR method of assay was established with high specificity, and good reproducibility. By optimizing the PCR condition, the optimal annealing temperature was 55℃, and the lowest detection limit was 160 pg. No bands were found in non-specificity samples (HAV, HBV, HCV and TB), and the sequences of PCR products were the same as the expected ones. Also a MBL PCR-RFLP was established. Upon electrophoresis of the digested products in 3% agarose gel, there were 3 patterns: in which 2 bands correspond to molecule weight 232 bp and 93 bp; 1 band, corresponds to molecule weight 325 bp and 3 bands correspond to molecule weight 325 bp, 232 bp and 93 bp, respectively. Three bands of 325 bp, 232 bp and 93 bp of point mutations were found at codon 54 of MBL coding gene. Frequencies in healthy Han and Mongolian population were 0.2321 and 0.1757 respectively. The average plasma MBL concentration was 1998.750 μg/L, with SD of 1505.152 in 56 healthy Han population and 2525.676 μg/L, with SD of 1955.188 in 37 Mongolian. A negative correlation between MBL concentration and gene mutation frequency was found in healthy Han population. Frequency of point mutation was 1.00 when the MBL concentrations were below 100 μg/L; frequency of point mutation was 0.4524 when the concentration was 100 μg/L to 1000 μg/L; and the frequency of point mutation was 0.0156 when the concentration was over 1000 μg/L. Analysis of association between MBL concentration and gene frequency in healthy Mongolian population showed that frequency of point mutation was 1.00 when the MBL concentrations were below 100 μg/L and the frequency of point mutation was 0.4583 when the MBL concentrations were 100 μg/L to 1000 μg/L; no point mutation was found when the concentration was over 1000 μg/L. It is concluded that the frequencies of mutation at codon 54 of MBL coding gene had been determined in both healthy Hans and Mongolian population, and the frequency was higher in healthy Hans than that of Mongolian, but no statistical significance (χ 2=0.8574, P >0.05). The MBL level was lower in healthy Hans than in Mongolian population, but there was no statistical significance( t =1.448, 0.1< P <0.2). There was a negative correlation between frequency of point mutation and MBL concentrations in both Hans and Mongolian population( r =-0.62, r =-0.641).
基金Supported by the National Natural Science Foundation of China(No.31370296)the Shandong Provincial Natural Science Foundation of China(Nos.Y2007D34,ZR2011CM044)the Key Technologies R&D Program of Shandong Province of China(No.2010GNC10937)
文摘Reactive oxygen species （ROS） scavengers, including ascorbate peroxidase, superoxide dismutase, catalase and peroxidase, are the most commonly used biomarkers in assessing an organisms＇ response to many biotic and abiotic stresses. In this study, we cloned an 866 bp GST（phi） gene in Lemna minor and investigated its characteristics, expression and enzymatic activities under 75 lamol/L cadmium concentrations in comparison with other ROS scavengers. GST（phi） gene expression patterns were similar to those of other scavengers of ROS. This suggests that GST（phi） might be involved in responding to heavy metal （cadmium） stress and that its expression level could be used as a bio-indicator in monitoring cadmium pollution.
基金covered by the Chinese PLA General Hospital,where the study was conducted.
文摘Background:White smoke bomb exposure in the military setting could result in organ injuries,which is uncommon and has been rarely described in previous studies.The aim of this study is to observe whether serum zinc levels are associated with liver function after white smoke inhalation.Methods:Fifteen patients with white smoke exposure were the subjects in this study.The clinical manifestations,liver function tests and the serum zinc levels were analysed.Results:The level of serum zinc was increased in the moderate or severe group(n=4)compared with that in the mild group(n=11).The four cases in the moderate or severe group had delayed impairment of liver function with an elevation of alanine transaminase.Additionally,increased blood concentrations of hyaluronic acid were found in three patients in the moderate or severe group.But no hexachloroethane or trichloropropane was detected.Conclusion:This study suggested that serum zinc levels may be associated with the severity of liver injuries after white smoke inhalation.
