目的:筛选气血双补方中抗慢性心力衰竭的主要活性成分,预测活性成分的作用靶点,构建“单味药-活性成分-作用靶点”网络,进一步探讨气血双补方抗心力衰竭的潜在作用机制。方法:采用中药系统药理学分析平台(traditional Chinese medicine ...目的:筛选气血双补方中抗慢性心力衰竭的主要活性成分,预测活性成分的作用靶点,构建“单味药-活性成分-作用靶点”网络,进一步探讨气血双补方抗心力衰竭的潜在作用机制。方法:采用中药系统药理学分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)检索气血双补方中各单味中药相关的所有化学成分、作用靶点,进而构建化合物-靶点网络图。通过TTD数据库、STRING数据库及UniProt数据库筛选慢性心力衰竭相关的靶点,进而构建疾病靶点相互作用网络图。筛选药物靶点和疾病靶点相互作用图的核心靶点,利用ClueGO对核心靶点进行GO分析,利用DAVID和KEGG数据库对核心靶点进行相关通路的富集。结果:选择口服利用度(oral bioavailability,OB)≥30%,类药性(drug-likeness,DL)≥0.18作为化合物分子的筛选条件,筛选出气血双补方中的107个活性成分和177个相应的蛋白靶点。通过度、介度中心度、接近中心度等网络拓扑特征评价、筛选出与气血双补方抗慢性心力衰竭作用的核心靶点190个,GO分析共包括256条富集结果,其中分子功能49条,生物过程181条,细胞组成26条。利用KEGG数据库对相关通路富集,筛选出20条通路在慢性心力衰竭方面具有作用。结论:通过网络药理学验证了气血双补方多成分、多靶点、整体调节的作用特点,预测了气血双补方抗慢性心力衰竭的主要作用机制,为其活性成分研究和实验研究提供了科学的理论依据。展开更多
Objective： To observe the effect of Yiqixue Buganshen recipe （益气血补肝肾方, YBR） on the expression of integrin e~ v 13 3 in the endometrium of controlled ovarian hyperstimulation mice. Methods： A total of 180 mi...Objective： To observe the effect of Yiqixue Buganshen recipe （益气血补肝肾方, YBR） on the expression of integrin e~ v 13 3 in the endometrium of controlled ovarian hyperstimulation mice. Methods： A total of 180 mice were divided into three groups： model group, treatment group and control group. The treatment and model groups were intraperitoneally injected with gonadotropin-releasing hormone analogue for 7 days; pregnant mare serum gonadotropin was also injected on the 7th day. After 48 h, human chorionic gonadotropin was injected. The control group was injected with an equal volume of saline at the same time. From the start of the experiment, the treatment group was intragastrically administered Jinghouzengzhi Recipe （经后增殖方） and Cuhuangti Recipe （促黄体方）. The model group and the control group were intragastrically administered an equal volume of saline. Real-time reverse transcription polymerase chain reaction and Western blotting were used to detect the mRNA and protein expression of integrin α υ β 3 in mouse endometrium. Results： Integrin α υ β 3 was expressed in mouse endometrium in all groups. Integrin α υ β 3 expression increased gradually along with pregnancy, progressing from pregnant day （Pd） 1. Integrin α υ β 3 expression significantly increased on Pd 4, then began to decrease on Pd 6. Integrin α υ β 3 expression in the treatment group was higher than in the model group, and the difference was statistically significant （P〈0.05）. The difference between the treatment group and the control group was not statistically significant （P〉0.05）. Conclusion： YBR improves endometrial receptivity, and may play an important role in embryonic implantation.展开更多
文摘目的:筛选气血双补方中抗慢性心力衰竭的主要活性成分,预测活性成分的作用靶点,构建“单味药-活性成分-作用靶点”网络,进一步探讨气血双补方抗心力衰竭的潜在作用机制。方法:采用中药系统药理学分析平台(traditional Chinese medicine systems pharmacology database and analysis platform,TCMSP)检索气血双补方中各单味中药相关的所有化学成分、作用靶点,进而构建化合物-靶点网络图。通过TTD数据库、STRING数据库及UniProt数据库筛选慢性心力衰竭相关的靶点,进而构建疾病靶点相互作用网络图。筛选药物靶点和疾病靶点相互作用图的核心靶点,利用ClueGO对核心靶点进行GO分析,利用DAVID和KEGG数据库对核心靶点进行相关通路的富集。结果:选择口服利用度(oral bioavailability,OB)≥30%,类药性(drug-likeness,DL)≥0.18作为化合物分子的筛选条件,筛选出气血双补方中的107个活性成分和177个相应的蛋白靶点。通过度、介度中心度、接近中心度等网络拓扑特征评价、筛选出与气血双补方抗慢性心力衰竭作用的核心靶点190个,GO分析共包括256条富集结果,其中分子功能49条,生物过程181条,细胞组成26条。利用KEGG数据库对相关通路富集,筛选出20条通路在慢性心力衰竭方面具有作用。结论:通过网络药理学验证了气血双补方多成分、多靶点、整体调节的作用特点,预测了气血双补方抗慢性心力衰竭的主要作用机制,为其活性成分研究和实验研究提供了科学的理论依据。
基金Supported by the National Natural Science Foundation of China(No.30973929)
文摘Objective： To observe the effect of Yiqixue Buganshen recipe （益气血补肝肾方, YBR） on the expression of integrin e~ v 13 3 in the endometrium of controlled ovarian hyperstimulation mice. Methods： A total of 180 mice were divided into three groups： model group, treatment group and control group. The treatment and model groups were intraperitoneally injected with gonadotropin-releasing hormone analogue for 7 days; pregnant mare serum gonadotropin was also injected on the 7th day. After 48 h, human chorionic gonadotropin was injected. The control group was injected with an equal volume of saline at the same time. From the start of the experiment, the treatment group was intragastrically administered Jinghouzengzhi Recipe （经后增殖方） and Cuhuangti Recipe （促黄体方）. The model group and the control group were intragastrically administered an equal volume of saline. Real-time reverse transcription polymerase chain reaction and Western blotting were used to detect the mRNA and protein expression of integrin α υ β 3 in mouse endometrium. Results： Integrin α υ β 3 was expressed in mouse endometrium in all groups. Integrin α υ β 3 expression increased gradually along with pregnancy, progressing from pregnant day （Pd） 1. Integrin α υ β 3 expression significantly increased on Pd 4, then began to decrease on Pd 6. Integrin α υ β 3 expression in the treatment group was higher than in the model group, and the difference was statistically significant （P〈0.05）. The difference between the treatment group and the control group was not statistically significant （P〉0.05）. Conclusion： YBR improves endometrial receptivity, and may play an important role in embryonic implantation.
