本试验考察了土壤温度、有机质含量和pH对海洋生防细菌多粘类芽胞杆菌L_1-9在黄瓜根表土壤中定殖的影响。采用实时荧光定量PCR方法检测了不同时期黄瓜根表土壤中菌株L_1-9的16S r DNA拷贝数。结果表明,土壤有机质含量、土壤温度和pH对菌...本试验考察了土壤温度、有机质含量和pH对海洋生防细菌多粘类芽胞杆菌L_1-9在黄瓜根表土壤中定殖的影响。采用实时荧光定量PCR方法检测了不同时期黄瓜根表土壤中菌株L_1-9的16S r DNA拷贝数。结果表明,土壤有机质含量、土壤温度和pH对菌株L_1-9在黄瓜根表的定殖有显著影响(P<0.01)。25℃定殖数量最高,为5.79×108拷贝/g土,是15℃时的3.4倍;添加有机肥20~40 g/kg土壤,可以促进菌株L_1-9在黄瓜根际定殖;pH 7时,菌株L_1-9在黄瓜根表土壤中的定殖数量最高。本研究为海洋生防菌L_1-9防治黄瓜土传病害提供了理论依据。展开更多
Under the condition of high temperature, the effects of five cover patterns (clean tillage, film mulching, weed covering, branches and leaves covering and growing grass covering) on soil properties in young Phoebe b...Under the condition of high temperature, the effects of five cover patterns (clean tillage, film mulching, weed covering, branches and leaves covering and growing grass covering) on soil properties in young Phoebe boumei forest were in- vestigated. The results showed that the five cover patterns all showed significant ef- fects on soil properties in young Phoebe bournei forest under the condition of high temperature. Land cover increased land temperature in Phoebe boumei forest. Un- der the film mulching, the land temperature was increased most rapidly with the largest increment. However, weed covering, branches and leaves covering and growing grass covering decreased land temperature. Among them, growing grass covering showed the best cooling effect. The film mulching, weed covering, branch- es and leaves covering and growing grass covering all increased land humidity. The film mulching showed the best moisture-preserving effect in the early period, but in the late period, the humidity in the film mulching treatment group was lower than that in the clean tillage treatment group. Among the five mulching patterns, moisture loss in the film mulching treatment group was slowest and least, followed by those in the weed covering and branches and leaves covering treatment groups, and moisture loss in the growing grass covering treatment group was fastest and most.展开更多
[Objective]The research aimed to study the effects of vegetation coverage on the changes of soil moisture in rainy season in dry-hot valley.[Method]The surface runoff and soil moisture of slope with vegetation coverag...[Objective]The research aimed to study the effects of vegetation coverage on the changes of soil moisture in rainy season in dry-hot valley.[Method]The surface runoff and soil moisture of slope with vegetation coverage and bare land in rainy reason in Jinsha River at Yuanmou County of Yunnan Province were observed continuously.Moreover,the statistical analysis was made based on the observation data.[Result]The vegetation coverage could decrease surface runoff and the surface runoff on bare land(CK) was 22 times as the plot with vegetation coverage.The soil water content in 0-180 cm layer with vegetation coverage increased by 37.8% than bare land.The stability of soil moisture content in deep layer was enhanced and the physical properties stability of soil was maintained.The soil moisture content in different depth of soil had significant difference and the changes of soil moisture content were obviously different.[Conclusion]The vegetation coverage of slope could change the soil hydrology obviously and keep soil moisture at the higher level,especially at soil layer below 20 cm.展开更多
Different ground mulching treatments were designed to observe the effects on soil moisture content in a second-generation seed orchard of Pinus elliottii. The results showed that: (1) there were extreme differences...Different ground mulching treatments were designed to observe the effects on soil moisture content in a second-generation seed orchard of Pinus elliottii. The results showed that: (1) there were extreme differences in soil moisture content Ⅰ and soil moisture content Ⅲ, but no obvious difference in soil moisture content II was observed; and (2) the high-to-low order of soil moisture content Ⅰ under different ground mulching treatments was white film〉sawdust〉black film〉the control, and the highest value was higher than the lowest one by 26.69%; the high-to-low order of soil moisture content Ⅱ under different ground mulching treatments was white film〉black film〉sawdust〉the control, and the highest value was higher than the lowest one by 20.64%; and the high-to-low order of soil moisture content Ⅲ under different ground mulching treatments was black film〉white film〉sawdust〉the control, and the highest value was higher than the lowest one by 26.61%.展开更多
Calamagrostis epigejos (L.) Roth. is a perennial grass with slender and long rhizome segments between interconnected neighbor ramets. To investigate the phenotypic plasticity in response to the heterogeneous soil wate...Calamagrostis epigejos (L.) Roth. is a perennial grass with slender and long rhizome segments between interconnected neighbor ramets. To investigate the phenotypic plasticity in response to the heterogeneous soil water supply, ramet pairs of the species were subjected to heterogeneous water supply by which either mother ramets or daughter ramets were in high or low soil water supply, respectively, in the Maowusu (Mu Us) Sandy Land of Nei Mongol. The results showed that the phenotypic characteristics of the individual ramets of C epigejos were greatly influenced by the heterogeneous water supply. The ramets treated with high water supply significantly produced more new rhizomes and more offspring (ramets), and accumulated more shoot biomass, and allocated more biomass to their shoots than those treated with low water supply. In comparison with the daughter ramets in homogeneous soil water supply, phenotypic characteristics, in terms of new rhizome growth, the production of new offspring, and the biomass allocation pattern, of the daughter ramets within the pairs of the species were not significantly changed, no matter that high or low soil water supply to mother ramets. The phenotypic responses of mother ramets to soil water supply were similar to those of daughter ramets. From these results, it is inferred that the interconnected ramets of C epigejos response phenotypically to their local soil water rather than to the soil water experienced by the interconnected ramets. The interconnected ramets of C epigejos might be independent of each other in water relationship, although they are physically interconnected with rhizome segments. The physiological independence of interconnected ramets might facilitate the risk spreading and thus enhance the genet survivorship under the frequent drought stresses in Mu Us Sandland.展开更多
Cotton leaf curl virus (CLCuV) belongs to the subgroup III of geminiviruses with single strand DNA genome. Study demonstrated that the bidirectional promoter of CLCuV had activity in Agrobacterium tumefaciens (Smith e...Cotton leaf curl virus (CLCuV) belongs to the subgroup III of geminiviruses with single strand DNA genome. Study demonstrated that the bidirectional promoter of CLCuV had activity in Agrobacterium tumefaciens (Smith et Townsend) Conn. This is the first report for the activity of the bidirectional promoter of geminivirus in A. tumefaciens. Results showed that the activity of the complementary sense promoter was stronger than that of virion sense promoter, and was detected 2-fold higher than that of CaMV 35S promoter in A. tumefaciens. Moreover, the promoter 5' deletion analysis indicated that the mean GUS activity driven by a 287 nucleotides complementary sense promoter fragment (from-287 to the translation initiation site) is 4 times higher than that driven by the whole complementary sense promoter in A. tumefaciens. This result suggested that there might exist negative regulatory elements in this deleted fragment. The function of other cis-elements included in CLCuV complementary sense promoter was also discussed in this paper.展开更多
The presence of a thatch layer in established pastures could reduce the contact between broiler litter and soil, thus increasing the potential for surface runoff contamination with litter P. We conducted a laboratory ...The presence of a thatch layer in established pastures could reduce the contact between broiler litter and soil, thus increasing the potential for surface runoff contamination with litter P. We conducted a laboratory study to evaluate the effect of a thatch layer on the dynamics of water-soluble P in undisturbed cores taken from a pasture. Cores with and without a thatch layer received a surface application of broiler litter (5 thm-2) and were incubated at 25 oC for 56 d. The result showed that on the soil surface the contents of water soluble-P (39 kghm-2) of the cores with the thatch layer was higher than that (20 kghm-2) of the cores without the thatch layer. Therefore on well-established pastures fertilized with broiler litter, the presence of a thatch layer might lead to high concentrations of water-soluble P on the soil surface.展开更多
The research progress of soil loss under the dual structure of southwest karst is systematically studied. The results show that the research of the soil erosion in karst mountainous area started late, and the basic re...The research progress of soil loss under the dual structure of southwest karst is systematically studied. The results show that the research of the soil erosion in karst mountainous area started late, and the basic research is lagging. Most of the existing research results focus on the present situation, causes and control measures of surface erosion. The view of underground soil loss in the context of karst diploid structure has been recognized by most scholars. However, limited to the research methods and the lack of observational data, the way of underground soil loss, the amount of loss and its harm are still unclear. Therefore, seeking the necessary technical means to carry out the necessary field observation from the way and process of loss is the focus of the study of soil loss under karst structure in the future.展开更多
[Objective] This study aimed to investigation the effects of straw covering and different types of potassium fertilizer on salinity accumulation in surface layer of tobacco-planted paddy soil in southern China. [Metho...[Objective] This study aimed to investigation the effects of straw covering and different types of potassium fertilizer on salinity accumulation in surface layer of tobacco-planted paddy soil in southern China. [Method] Tobacco variety ‘Yunyan87’ was used as the experimental material to investigate the effects of salinity accumulation in surface layer of tobacco-planted paddy soil on the growth and development of flue-cured tobacco using different types of potassium fertilizer and mulching cultivation methods. [Result] The results showed that K+ , Ca2+ , SO42and NO3-were the major salt ions in topsoil at different growth stages of flue-cured tobacco, Na + and Mg2+ contents were also relatively high at vigorous growth stage, indicating that these salt ions were easily accumulated in surface layer of soil; to be specific, the absolute increase of salt ion concentration showed a decreasing order of K+ SO42- NO3-Ca2+ Mg2+ Na+ Cl-, while the relative increase of salt ion concentration showed a decreasing order of Ca2+ K+ Na+ NO3-SO42-Mg2+ Cl-. At 60 d posttransplanting, total salt content in topsoil reached the minimum of 359.1 mg/kg in Treatment 2, total salt content in topsoil reached the maximum of 536.1 mg/kg in Treatment 5 (CK), which was significantly higher than that in other treatments. At 90 d post-transplanting, no significant difference was observed in total salt content among various treatments. At harvesting period, total salt content in topsoil reached the maximum of 3 278.4 mg/kg in Treatment 1, which was significantly higher than that in other treatments. Topsoil pH showed no significant differences among various treatments at three different periods, ranging from 5.39 to 5.59. Straw covering could effectively reduce salt content in topsoil, accelerate vigorous growth of tobacco, shorten vigorous growth period and increase plant height, leaf number and lead area; at vigorous growth stage, root vitality and root volume of tobacco were improved, but the yield and output value were relatively low. Major agronomic traits and yield of tobacco showed no significant difference among various treatments. Output value of tobacco reached the maximum of 24 196.8 yuan/hm2 in Treatment 3, which was significantly higher than that in other treatments. [Conclusion] Appropriate types and proportions of potassium fertilizer and straw covering can effectively reduce the total salt content in tobacco-planted paddy soil and increase the effective supply amount of K+ , Ca2+ , SO42-and NO3-, thereby promoting and improving the root vitality of tobacco, which is conducive to the growth and development of tobacco and will eventually enhance the yield, quality and economic benefits of flue-cured tobacco.展开更多
Effects of factors such as slope, surface soil textuie, fertilization andcrop cover with different rainfall intensities on phosphorus (P) losses in farmland runoff of theDianchi Lake Watershed in Yunnan Province of Ch...Effects of factors such as slope, surface soil textuie, fertilization andcrop cover with different rainfall intensities on phosphorus (P) losses in farmland runoff of theDianchi Lake Watershed in Yunnan Province of China were studied through a rainfall simulation testusing a red soil, one of the most widely distributed soils of the study area. Results showed thatthe runoff concentrations of total phosphorus (TP) and P losses differed with the slope, beinghighest when the slope was 18 deg. At twodifferent rainfall intensities, the runoff TP and P losseshad a similar decreasing trend as the surface soil texture became coarser, therefore applying thegrit would decrease P in runofi from soils of farmland on slopes with heavier textures. With wheatas a crop cover the runoff TP concentrations and P losses were significantly lower than those of thebare soil. This showed that plant cover would greatly decrease P in runoff from the farmland of thestudy area. The TP concentration in runoff from the soil two days after fertilization doubled whencompared with that from the non-fertilized soil, indicating that fertilization could mean a dramaticrise in P runoff if irrigation or heavy rainfall occurred immediately after application and that nofertilization before a rain and no irrigation immediately after fertilization would reduce runoff Ploss from the farmland of the study area.展开更多
The effects of reforestation on carbon (C) sequestration in China's Loess Plateau ecosystem have attracted much research attention in recent years. Black locust trees (Robinia pseudoacacia L.) are valued for thei...The effects of reforestation on carbon (C) sequestration in China's Loess Plateau ecosystem have attracted much research attention in recent years. Black locust trees (Robinia pseudoacacia L.) are valued for their important use in reforestation and water and soil conservation efforts. This forest type is widespread across the Loess Plateau, and must he an essential component of any planning for C sequestration efforts in this fragile ecological region. The long-term effects of stand age on C accumulation and allocation after reforestation remains uncertain. We examined an age-sequence of black locust forest (5, 9, 20, 30, 38, and 56 yr since planting) on the Loess Plateau to evaluate C accumulation and allocation in plants (trees, shrubs, herbages, and leaf litter) and soil (0-100 cm). Allometric equations were developed for estimating the biomass of tree components (leaf, branch, stem without bark, bark and root) with a de- structive sampling method. Our results demonstrated that black locust forest ecosystem accumulated C constantly, from 31.42 Mg C/ha (1 Mg = 106 g) at 5 yr to 79.44 Mg C/haat 38 yr. At the 'old forest' stage (38 to 56 yr), the amount of C in plant biomass significantly decreased (from 45.32 to 34.52 Mg C/ha) due to the high mortality of trees. However, old forest was able to accumulate C continuously in soil (from 33.66 to 41.00 Mg C/ha). The C in shrub biomass increased with stand age, while the C stock in the herbage layer and leaf litter was age-independent. Reforestation resulted in C re-allocation in the forest soil. The topsoil (0-20 cm) C stock increased constantly with stand age. However, C storage in sub-top soil, in the 20-30, 30-50, 50-100, and 20-100 cm layers, was age-independent. These results suggest that succession, as a temporal factor, plays a key role in C accumulation and re-allocation in black locust forests and also in regional C dynamics in vegetation.展开更多
A soil batch experiment was conducted to investigate both separate and compound effects of three types of surfactants: anionic dodecylbenzene sulfonic acid sodiumsalt (DBSS), cationic cetyltrimethylammonium bromide (C...A soil batch experiment was conducted to investigate both separate and compound effects of three types of surfactants: anionic dodecylbenzene sulfonic acid sodiumsalt (DBSS), cationic cetyltrimethylammonium bromide (CTAB), and non-ionic nonyl phenol polyethyleneoxy ether (TX-100), as well as ethylenediaminetetraacetic acid (EDTA) on cadmium solubility, sorption kinetics, and sorption-desorption behavior in purple soil. The results indicated that both individual application of the three types of surfactants and surfactants combined with EDTA could stimulate Cd extraction from the soil with a general effectiveness ranking of EDTA/TX-100 > EDTA/DBSS > EDTA/CTAB > EDTA > TX-100 > DBSS > CTAB. Further study showed that the compound application of surfactants and EDTA had stronger (P < 0.05) effects on Cd solubility than those added individually. The application of surfactants and EDTA to purple soil (P < 0.05) decreased the proportion of Cd sorbed, while their effectiveness ranking was similar to that of enhanced solubilization. The sorption kinetics of Cd in purple soil was best described by the double-constant equation, while the Freundlich equation gave an excellent fit to the sorption isotherm curves. Therefore, surfactant-enhanced remediation of Cd contaminated soil is feasible and further research should be conducted.展开更多
Global nitrogen (N) budgets for intensive agricultural systems were compiled for a 0.5 by 0.5 degree resolution. These budgets include N inputs (N fertilizer, animal manure, biological N fixation and atmospheric N dep...Global nitrogen (N) budgets for intensive agricultural systems were compiled for a 0.5 by 0.5 degree resolution. These budgets include N inputs (N fertilizer, animal manure, biological N fixation and atmospheric N deposition) and outputs (N removal from the field in harvested crops and grass and grass consumption by grazing animals, ammonia volatilization,denitrification and leaching). Data for the historical years 1970 and 1995 and a projection for 2030 were used to study changes in the recovery of N and the different loss terms for intensive agricultural systems. The results indicate that the overall system N recovery and fertilizer use efficiency slowly increased in the industrialized countries between 1970 and 1995, the values for developing countries have decreased in the same period. For the coming three decades our results indicate a rapid increase in both the industrialized and developing countries. High values of > 80% for fertilizer use efficiency may be related to surface N balance deficits, implying a depletion of soil N and loss of soil fertility. The projected intensification in most developing countries will cause a gradual shift from deficits to surpluses in the coming decades.The projected fast growth of crop and livestock production, and intensification and associated increase in fertilizer inputs will cause a major increase in the surface N balance surplus in the coming three decades. This implies increasing losses of N compounds to air (ammonia, nitrous oxide and nitric oxide), and groundwater and surface water (nitrate).展开更多
Soil samples were taken from an Ermans birch (Betula ermanii)-dark coniferous forest (Picea jezoensis and Abies nephrolepis) ecotone growing on volcanic ejecta in the northern slope of Changbai Mountains of Northe...Soil samples were taken from an Ermans birch (Betula ermanii)-dark coniferous forest (Picea jezoensis and Abies nephrolepis) ecotone growing on volcanic ejecta in the northern slope of Changbai Mountains of Northeast China, to compare soil carbon (C) and nitrogen (N) transformations in the two forests. The soil type is Umbri-Gelic Cambosols in Chinese Soil Taxonomy. Soil samples were incubated aerobically at 20℃ and field capacity of 700 g kg^-1 over a period of 27 weeks. The amount of soil microbial biomass and net N mineralization were higher in the Ermans birch than the dark coniferous forest (P 〈 0.05), whereas the cumulative C mineralization (as CO2 emission) in the dark coniferous forest exceeded that in the Ermans birch (P 〈 0.05). Release of the cumulative dissolved organic C and dissolved organic N were greater in the Ermans birch than the dark coniferous forest (P 〈 0.05). The results suggested that differences of forest types could result in considerable change in soil C and N transformations.展开更多
Rapid urbanization results in the conversion of natural soil to urban soil,and consequently,the storage and density of the soil carbon pools change.Taking Chongqing Municipality of China as a study case,this investiga...Rapid urbanization results in the conversion of natural soil to urban soil,and consequently,the storage and density of the soil carbon pools change.Taking Chongqing Municipality of China as a study case,this investigation attempts to better understand soil carbon pools in hilly cities.First,the vegetated areas in the study area were derived from QuickBird images.Then,topsoil data from 220 soil samples(0-20 cm) in the vegetated areas were collected and their soil organic carbon(SOC) densities were analyzed.Using the Kriging interpolation method,the spatial pattern of SOC was estimated.The results show that the SOC density exhibited high spatial variability in the urban topsoil of Chongqing.First,the SOC density in topsoil decreased according to slope in the order 2°-6° < 25°-90° < 0°-2° < 6°-15° < 15°-25°.Second,the newly developed areas during 2001-2010 had a lower SOC density than the areas built before 1988.Third,urban parks and gardens had a higher SOC density in topsoil,residential green land followed,and scattered street green land ranked last.For hilly cities,the variability of terrain affects the distribution of SOC.The Kriging results indicate that Kriging method combining slope with SOC density produced a high level of accuracy.The Kriging results show that the SOC density to the north of the Jialing River was higher than the south.The vegetated areas were estimated to amount to 73.5 km2 across the study area with an SOC storage of 0.192 Tg and an average density of 2.61 kg/m2.展开更多
Erosion agents and patterns profoundly affect hillslope soil loss characteristics. However, few attempts have been made to analyze the effects of rainfall and inflow on soil erosion for hillslopes dominated by sheet e...Erosion agents and patterns profoundly affect hillslope soil loss characteristics. However, few attempts have been made to analyze the effects of rainfall and inflow on soil erosion for hillslopes dominated by sheet erosion or rill erosion in the Chinese Mollisol region. The objective of this study was to discuss the erosive agent(rainfall or inflow), hillslope erosion pattern(sheet erosion or rill erosion) and slope gradient effects on runoff and soil losses. Two soil pans(2.0 m long, 0.5 m wide and 0.5 m deep) with 5° and 10° slopes were subjected to rainfall(0 and 70 mm h–1) and inflow(0 and 70 mm h–1) experiments. Three experimental combinations of rainfall intensity(RI) and inflow rate(IR) were tested using the same water supply of 70 mm by controllingthe run time. A flat soil surface and a soil bed with a straight initial rill were prepared manually, and represented hillslopes dominated by sheet erosion and rill erosion, respectively. The results showed that soil losses had greater differences among treatments than total runoff. Soil losses decreased in the order of RI70+IR70 > RI70+IR0 > RI0+IR70. Additionally, soil losses for hillslopes dominated by rill erosion were 1.7-2.2 times greater at 5° and 2.5-6.9 times greater at 10° than those for hillslopes dominated by sheet erosion. The loss of <0.25 mm soil particles and aggregates varying from 47.72%-99.60% of the total soil loss played a dominant role in the sediment. Compared with sheet erosion hillslopes, rill erosion hillslopes selectively transported more microaggregates under a relatively stable rill development stage, but rills transported increasinglymore macroaggregates under an active rill development stage. In conclusion, eliminating raindrop impact on relatively gentle hillslopes and preventing rill development on relatively steep hillslopes would be useful measures to decrease soil erosion and soil degradation in the Mollisol region of northeastern China.展开更多
文摘本试验考察了土壤温度、有机质含量和pH对海洋生防细菌多粘类芽胞杆菌L_1-9在黄瓜根表土壤中定殖的影响。采用实时荧光定量PCR方法检测了不同时期黄瓜根表土壤中菌株L_1-9的16S r DNA拷贝数。结果表明,土壤有机质含量、土壤温度和pH对菌株L_1-9在黄瓜根表的定殖有显著影响(P<0.01)。25℃定殖数量最高,为5.79×108拷贝/g土,是15℃时的3.4倍;添加有机肥20~40 g/kg土壤,可以促进菌株L_1-9在黄瓜根际定殖;pH 7时,菌株L_1-9在黄瓜根表土壤中的定殖数量最高。本研究为海洋生防菌L_1-9防治黄瓜土传病害提供了理论依据。
基金Supported by Forestry Science and Technology Program of Hunan Province(XLK201406)~~
文摘Under the condition of high temperature, the effects of five cover patterns (clean tillage, film mulching, weed covering, branches and leaves covering and growing grass covering) on soil properties in young Phoebe boumei forest were in- vestigated. The results showed that the five cover patterns all showed significant ef- fects on soil properties in young Phoebe bournei forest under the condition of high temperature. Land cover increased land temperature in Phoebe boumei forest. Un- der the film mulching, the land temperature was increased most rapidly with the largest increment. However, weed covering, branches and leaves covering and growing grass covering decreased land temperature. Among them, growing grass covering showed the best cooling effect. The film mulching, weed covering, branch- es and leaves covering and growing grass covering all increased land humidity. The film mulching showed the best moisture-preserving effect in the early period, but in the late period, the humidity in the film mulching treatment group was lower than that in the clean tillage treatment group. Among the five mulching patterns, moisture loss in the film mulching treatment group was slowest and least, followed by those in the weed covering and branches and leaves covering treatment groups, and moisture loss in the growing grass covering treatment group was fastest and most.
基金Supported by National Key Project of Scientific and Technical Supporting Programs (2006BAC01A11 )National Natural Science Foundation of China (2006AA202A04)~~
文摘[Objective]The research aimed to study the effects of vegetation coverage on the changes of soil moisture in rainy season in dry-hot valley.[Method]The surface runoff and soil moisture of slope with vegetation coverage and bare land in rainy reason in Jinsha River at Yuanmou County of Yunnan Province were observed continuously.Moreover,the statistical analysis was made based on the observation data.[Result]The vegetation coverage could decrease surface runoff and the surface runoff on bare land(CK) was 22 times as the plot with vegetation coverage.The soil water content in 0-180 cm layer with vegetation coverage increased by 37.8% than bare land.The stability of soil moisture content in deep layer was enhanced and the physical properties stability of soil was maintained.The soil moisture content in different depth of soil had significant difference and the changes of soil moisture content were obviously different.[Conclusion]The vegetation coverage of slope could change the soil hydrology obviously and keep soil moisture at the higher level,especially at soil layer below 20 cm.
基金Supported by the "948" Program of State Forestry Administration(2013-4-37)~~
文摘Different ground mulching treatments were designed to observe the effects on soil moisture content in a second-generation seed orchard of Pinus elliottii. The results showed that: (1) there were extreme differences in soil moisture content Ⅰ and soil moisture content Ⅲ, but no obvious difference in soil moisture content II was observed; and (2) the high-to-low order of soil moisture content Ⅰ under different ground mulching treatments was white film〉sawdust〉black film〉the control, and the highest value was higher than the lowest one by 26.69%; the high-to-low order of soil moisture content Ⅱ under different ground mulching treatments was white film〉black film〉sawdust〉the control, and the highest value was higher than the lowest one by 20.64%; and the high-to-low order of soil moisture content Ⅲ under different ground mulching treatments was black film〉white film〉sawdust〉the control, and the highest value was higher than the lowest one by 26.61%.
文摘Calamagrostis epigejos (L.) Roth. is a perennial grass with slender and long rhizome segments between interconnected neighbor ramets. To investigate the phenotypic plasticity in response to the heterogeneous soil water supply, ramet pairs of the species were subjected to heterogeneous water supply by which either mother ramets or daughter ramets were in high or low soil water supply, respectively, in the Maowusu (Mu Us) Sandy Land of Nei Mongol. The results showed that the phenotypic characteristics of the individual ramets of C epigejos were greatly influenced by the heterogeneous water supply. The ramets treated with high water supply significantly produced more new rhizomes and more offspring (ramets), and accumulated more shoot biomass, and allocated more biomass to their shoots than those treated with low water supply. In comparison with the daughter ramets in homogeneous soil water supply, phenotypic characteristics, in terms of new rhizome growth, the production of new offspring, and the biomass allocation pattern, of the daughter ramets within the pairs of the species were not significantly changed, no matter that high or low soil water supply to mother ramets. The phenotypic responses of mother ramets to soil water supply were similar to those of daughter ramets. From these results, it is inferred that the interconnected ramets of C epigejos response phenotypically to their local soil water rather than to the soil water experienced by the interconnected ramets. The interconnected ramets of C epigejos might be independent of each other in water relationship, although they are physically interconnected with rhizome segments. The physiological independence of interconnected ramets might facilitate the risk spreading and thus enhance the genet survivorship under the frequent drought stresses in Mu Us Sandland.
文摘Cotton leaf curl virus (CLCuV) belongs to the subgroup III of geminiviruses with single strand DNA genome. Study demonstrated that the bidirectional promoter of CLCuV had activity in Agrobacterium tumefaciens (Smith et Townsend) Conn. This is the first report for the activity of the bidirectional promoter of geminivirus in A. tumefaciens. Results showed that the activity of the complementary sense promoter was stronger than that of virion sense promoter, and was detected 2-fold higher than that of CaMV 35S promoter in A. tumefaciens. Moreover, the promoter 5' deletion analysis indicated that the mean GUS activity driven by a 287 nucleotides complementary sense promoter fragment (from-287 to the translation initiation site) is 4 times higher than that driven by the whole complementary sense promoter in A. tumefaciens. This result suggested that there might exist negative regulatory elements in this deleted fragment. The function of other cis-elements included in CLCuV complementary sense promoter was also discussed in this paper.
文摘The presence of a thatch layer in established pastures could reduce the contact between broiler litter and soil, thus increasing the potential for surface runoff contamination with litter P. We conducted a laboratory study to evaluate the effect of a thatch layer on the dynamics of water-soluble P in undisturbed cores taken from a pasture. Cores with and without a thatch layer received a surface application of broiler litter (5 thm-2) and were incubated at 25 oC for 56 d. The result showed that on the soil surface the contents of water soluble-P (39 kghm-2) of the cores with the thatch layer was higher than that (20 kghm-2) of the cores without the thatch layer. Therefore on well-established pastures fertilized with broiler litter, the presence of a thatch layer might lead to high concentrations of water-soluble P on the soil surface.
基金Supported by the National Science&Technology Pillar Program during the Twelfth FiveYear Plan Period of China(2012BAD05B06)the Fund Program for Science and Technology of Guizhou in 2012(Qiankehe Code J LKS[2012]No.27~~
文摘The research progress of soil loss under the dual structure of southwest karst is systematically studied. The results show that the research of the soil erosion in karst mountainous area started late, and the basic research is lagging. Most of the existing research results focus on the present situation, causes and control measures of surface erosion. The view of underground soil loss in the context of karst diploid structure has been recognized by most scholars. However, limited to the research methods and the lack of observational data, the way of underground soil loss, the amount of loss and its harm are still unclear. Therefore, seeking the necessary technical means to carry out the necessary field observation from the way and process of loss is the focus of the study of soil loss under karst structure in the future.
基金Supported by Project of Guangdong Zhongyan Industry Limited Liability Company[YYG15JO-QK(2011)-004]~~
文摘[Objective] This study aimed to investigation the effects of straw covering and different types of potassium fertilizer on salinity accumulation in surface layer of tobacco-planted paddy soil in southern China. [Method] Tobacco variety ‘Yunyan87’ was used as the experimental material to investigate the effects of salinity accumulation in surface layer of tobacco-planted paddy soil on the growth and development of flue-cured tobacco using different types of potassium fertilizer and mulching cultivation methods. [Result] The results showed that K+ , Ca2+ , SO42and NO3-were the major salt ions in topsoil at different growth stages of flue-cured tobacco, Na + and Mg2+ contents were also relatively high at vigorous growth stage, indicating that these salt ions were easily accumulated in surface layer of soil; to be specific, the absolute increase of salt ion concentration showed a decreasing order of K+ SO42- NO3-Ca2+ Mg2+ Na+ Cl-, while the relative increase of salt ion concentration showed a decreasing order of Ca2+ K+ Na+ NO3-SO42-Mg2+ Cl-. At 60 d posttransplanting, total salt content in topsoil reached the minimum of 359.1 mg/kg in Treatment 2, total salt content in topsoil reached the maximum of 536.1 mg/kg in Treatment 5 (CK), which was significantly higher than that in other treatments. At 90 d post-transplanting, no significant difference was observed in total salt content among various treatments. At harvesting period, total salt content in topsoil reached the maximum of 3 278.4 mg/kg in Treatment 1, which was significantly higher than that in other treatments. Topsoil pH showed no significant differences among various treatments at three different periods, ranging from 5.39 to 5.59. Straw covering could effectively reduce salt content in topsoil, accelerate vigorous growth of tobacco, shorten vigorous growth period and increase plant height, leaf number and lead area; at vigorous growth stage, root vitality and root volume of tobacco were improved, but the yield and output value were relatively low. Major agronomic traits and yield of tobacco showed no significant difference among various treatments. Output value of tobacco reached the maximum of 24 196.8 yuan/hm2 in Treatment 3, which was significantly higher than that in other treatments. [Conclusion] Appropriate types and proportions of potassium fertilizer and straw covering can effectively reduce the total salt content in tobacco-planted paddy soil and increase the effective supply amount of K+ , Ca2+ , SO42-and NO3-, thereby promoting and improving the root vitality of tobacco, which is conducive to the growth and development of tobacco and will eventually enhance the yield, quality and economic benefits of flue-cured tobacco.
基金Project supported by the Applied and Basic Research Foundation of Yunnan Province (No. 1999C0011G).
文摘Effects of factors such as slope, surface soil textuie, fertilization andcrop cover with different rainfall intensities on phosphorus (P) losses in farmland runoff of theDianchi Lake Watershed in Yunnan Province of China were studied through a rainfall simulation testusing a red soil, one of the most widely distributed soils of the study area. Results showed thatthe runoff concentrations of total phosphorus (TP) and P losses differed with the slope, beinghighest when the slope was 18 deg. At twodifferent rainfall intensities, the runoff TP and P losseshad a similar decreasing trend as the surface soil texture became coarser, therefore applying thegrit would decrease P in runofi from soils of farmland on slopes with heavier textures. With wheatas a crop cover the runoff TP concentrations and P losses were significantly lower than those of thebare soil. This showed that plant cover would greatly decrease P in runoff from the farmland of thestudy area. The TP concentration in runoff from the soil two days after fertilization doubled whencompared with that from the non-fertilized soil, indicating that fertilization could mean a dramaticrise in P runoff if irrigation or heavy rainfall occurred immediately after application and that nofertilization before a rain and no irrigation immediately after fertilization would reduce runoff Ploss from the farmland of the study area.
基金Under the auspices of Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA05060300)
文摘The effects of reforestation on carbon (C) sequestration in China's Loess Plateau ecosystem have attracted much research attention in recent years. Black locust trees (Robinia pseudoacacia L.) are valued for their important use in reforestation and water and soil conservation efforts. This forest type is widespread across the Loess Plateau, and must he an essential component of any planning for C sequestration efforts in this fragile ecological region. The long-term effects of stand age on C accumulation and allocation after reforestation remains uncertain. We examined an age-sequence of black locust forest (5, 9, 20, 30, 38, and 56 yr since planting) on the Loess Plateau to evaluate C accumulation and allocation in plants (trees, shrubs, herbages, and leaf litter) and soil (0-100 cm). Allometric equations were developed for estimating the biomass of tree components (leaf, branch, stem without bark, bark and root) with a de- structive sampling method. Our results demonstrated that black locust forest ecosystem accumulated C constantly, from 31.42 Mg C/ha (1 Mg = 106 g) at 5 yr to 79.44 Mg C/haat 38 yr. At the 'old forest' stage (38 to 56 yr), the amount of C in plant biomass significantly decreased (from 45.32 to 34.52 Mg C/ha) due to the high mortality of trees. However, old forest was able to accumulate C continuously in soil (from 33.66 to 41.00 Mg C/ha). The C in shrub biomass increased with stand age, while the C stock in the herbage layer and leaf litter was age-independent. Reforestation resulted in C re-allocation in the forest soil. The topsoil (0-20 cm) C stock increased constantly with stand age. However, C storage in sub-top soil, in the 20-30, 30-50, 50-100, and 20-100 cm layers, was age-independent. These results suggest that succession, as a temporal factor, plays a key role in C accumulation and re-allocation in black locust forests and also in regional C dynamics in vegetation.
基金Project supported by the Foundation for University Key Teachers through the Education Committee of Chongqing,China (No. 110758).
文摘A soil batch experiment was conducted to investigate both separate and compound effects of three types of surfactants: anionic dodecylbenzene sulfonic acid sodiumsalt (DBSS), cationic cetyltrimethylammonium bromide (CTAB), and non-ionic nonyl phenol polyethyleneoxy ether (TX-100), as well as ethylenediaminetetraacetic acid (EDTA) on cadmium solubility, sorption kinetics, and sorption-desorption behavior in purple soil. The results indicated that both individual application of the three types of surfactants and surfactants combined with EDTA could stimulate Cd extraction from the soil with a general effectiveness ranking of EDTA/TX-100 > EDTA/DBSS > EDTA/CTAB > EDTA > TX-100 > DBSS > CTAB. Further study showed that the compound application of surfactants and EDTA had stronger (P < 0.05) effects on Cd solubility than those added individually. The application of surfactants and EDTA to purple soil (P < 0.05) decreased the proportion of Cd sorbed, while their effectiveness ranking was similar to that of enhanced solubilization. The sorption kinetics of Cd in purple soil was best described by the double-constant equation, while the Freundlich equation gave an excellent fit to the sorption isotherm curves. Therefore, surfactant-enhanced remediation of Cd contaminated soil is feasible and further research should be conducted.
基金Project supported by the Netherlands Environmental Assessment Agency, National Institute for Public Health and the Environment (No. S/550005/01/DD) the Canadian International Development Agency, Canada and the Chinese Academy of Sciences, China (No.KZCX2-413)
文摘Global nitrogen (N) budgets for intensive agricultural systems were compiled for a 0.5 by 0.5 degree resolution. These budgets include N inputs (N fertilizer, animal manure, biological N fixation and atmospheric N deposition) and outputs (N removal from the field in harvested crops and grass and grass consumption by grazing animals, ammonia volatilization,denitrification and leaching). Data for the historical years 1970 and 1995 and a projection for 2030 were used to study changes in the recovery of N and the different loss terms for intensive agricultural systems. The results indicate that the overall system N recovery and fertilizer use efficiency slowly increased in the industrialized countries between 1970 and 1995, the values for developing countries have decreased in the same period. For the coming three decades our results indicate a rapid increase in both the industrialized and developing countries. High values of > 80% for fertilizer use efficiency may be related to surface N balance deficits, implying a depletion of soil N and loss of soil fertility. The projected intensification in most developing countries will cause a gradual shift from deficits to surpluses in the coming decades.The projected fast growth of crop and livestock production, and intensification and associated increase in fertilizer inputs will cause a major increase in the surface N balance surplus in the coming three decades. This implies increasing losses of N compounds to air (ammonia, nitrous oxide and nitric oxide), and groundwater and surface water (nitrate).
基金Project supported by the National Natural Science Foundation of China(No.90411020)the National Key Basic Research Program(973 Program)of China(No.2002CB412502)
文摘Soil samples were taken from an Ermans birch (Betula ermanii)-dark coniferous forest (Picea jezoensis and Abies nephrolepis) ecotone growing on volcanic ejecta in the northern slope of Changbai Mountains of Northeast China, to compare soil carbon (C) and nitrogen (N) transformations in the two forests. The soil type is Umbri-Gelic Cambosols in Chinese Soil Taxonomy. Soil samples were incubated aerobically at 20℃ and field capacity of 700 g kg^-1 over a period of 27 weeks. The amount of soil microbial biomass and net N mineralization were higher in the Ermans birch than the dark coniferous forest (P 〈 0.05), whereas the cumulative C mineralization (as CO2 emission) in the dark coniferous forest exceeded that in the Ermans birch (P 〈 0.05). Release of the cumulative dissolved organic C and dissolved organic N were greater in the Ermans birch than the dark coniferous forest (P 〈 0.05). The results suggested that differences of forest types could result in considerable change in soil C and N transformations.
基金Under the auspices of the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20090182120024)National Natural Science Foundation of China (No. 41101568)+1 种基金Natural Science Foundation Project of Chongqing Science & Technology Commission (No. cstcjjA00008)Fundamental Research Funds for the Central Universities (2012XZZX012)
文摘Rapid urbanization results in the conversion of natural soil to urban soil,and consequently,the storage and density of the soil carbon pools change.Taking Chongqing Municipality of China as a study case,this investigation attempts to better understand soil carbon pools in hilly cities.First,the vegetated areas in the study area were derived from QuickBird images.Then,topsoil data from 220 soil samples(0-20 cm) in the vegetated areas were collected and their soil organic carbon(SOC) densities were analyzed.Using the Kriging interpolation method,the spatial pattern of SOC was estimated.The results show that the SOC density exhibited high spatial variability in the urban topsoil of Chongqing.First,the SOC density in topsoil decreased according to slope in the order 2°-6° < 25°-90° < 0°-2° < 6°-15° < 15°-25°.Second,the newly developed areas during 2001-2010 had a lower SOC density than the areas built before 1988.Third,urban parks and gardens had a higher SOC density in topsoil,residential green land followed,and scattered street green land ranked last.For hilly cities,the variability of terrain affects the distribution of SOC.The Kriging results indicate that Kriging method combining slope with SOC density produced a high level of accuracy.The Kriging results show that the SOC density to the north of the Jialing River was higher than the south.The vegetated areas were estimated to amount to 73.5 km2 across the study area with an SOC storage of 0.192 Tg and an average density of 2.61 kg/m2.
基金funded by the National Natural Science Foundation of China(Grant Nos.41601281,41701313)the National Key R&D Program of China(Grant No.2016YFE0202900)
文摘Erosion agents and patterns profoundly affect hillslope soil loss characteristics. However, few attempts have been made to analyze the effects of rainfall and inflow on soil erosion for hillslopes dominated by sheet erosion or rill erosion in the Chinese Mollisol region. The objective of this study was to discuss the erosive agent(rainfall or inflow), hillslope erosion pattern(sheet erosion or rill erosion) and slope gradient effects on runoff and soil losses. Two soil pans(2.0 m long, 0.5 m wide and 0.5 m deep) with 5° and 10° slopes were subjected to rainfall(0 and 70 mm h–1) and inflow(0 and 70 mm h–1) experiments. Three experimental combinations of rainfall intensity(RI) and inflow rate(IR) were tested using the same water supply of 70 mm by controllingthe run time. A flat soil surface and a soil bed with a straight initial rill were prepared manually, and represented hillslopes dominated by sheet erosion and rill erosion, respectively. The results showed that soil losses had greater differences among treatments than total runoff. Soil losses decreased in the order of RI70+IR70 > RI70+IR0 > RI0+IR70. Additionally, soil losses for hillslopes dominated by rill erosion were 1.7-2.2 times greater at 5° and 2.5-6.9 times greater at 10° than those for hillslopes dominated by sheet erosion. The loss of <0.25 mm soil particles and aggregates varying from 47.72%-99.60% of the total soil loss played a dominant role in the sediment. Compared with sheet erosion hillslopes, rill erosion hillslopes selectively transported more microaggregates under a relatively stable rill development stage, but rills transported increasinglymore macroaggregates under an active rill development stage. In conclusion, eliminating raindrop impact on relatively gentle hillslopes and preventing rill development on relatively steep hillslopes would be useful measures to decrease soil erosion and soil degradation in the Mollisol region of northeastern China.