This study investigated the bacterial diversity of gut content of sea cucumber （Apostichopus japonicus） and its habitat surface sediment in a bottom enhancement area using PCR-based denaturing gradient gel electroph...This study investigated the bacterial diversity of gut content of sea cucumber （Apostichopus japonicus） and its habitat surface sediment in a bottom enhancement area using PCR-based denaturing gradient gel electrophoresis （DGGE） technique. Bacte- rial diversity evaluation showed that the value of the Shannon-Wiener index of gut content in different intestinal segments of A. ja-ponicus varied between 2.88 and 3.00, lower than that of the surrounding sediment （3.23）. Phyl0genetic analysis showed that bacte- rial phylotypes in gut content and the surrounding sediment ofA. japonicus were closely related to Proteobacteria including3,-, ct-, 8- and e-proteobacteria, Bacteroidetes, Firmicute, and Actinobacteria, of which 7-proteobacteria were predominant. These results sug- gested that the sea cucumber A. japonicus was capable of feeding selectively, and PCR-DGGE was applicable for characterizing the bacterial community composition in gut content and the surrounding sediment of sea cucumber. Further investigation targeting longer 16S rDNA gene fragments and/or functional genes was recommended for obtaining more information of the diversity and function of bacterial community in the gut content of sea cucumber.展开更多
The surface microlayer(SML)is a thin biogenic film found between the marine environment and troposphere that comprises a unique marine ecosystem with its own physicochemical and microbiological compositions.In this st...The surface microlayer(SML)is a thin biogenic film found between the marine environment and troposphere that comprises a unique marine ecosystem with its own physicochemical and microbiological compositions.In this study,we compared the bacterial cell abundance and community structure of the SML and subsurface waters(0.50m depth)using traditional culture and flow cytometry methods.Overall,53strains were screened and sequenced.In addition,DNA was extracted from the SML and corresponding subsurface water and used for bacterial community analysis by denaturing gradient gel electrophoresis(DGGE).The results revealed that the SML had a distinct microbial community structure from the corresponding subsurface water.This is the first compresensively detailed molecular biological analysis of the bacterioneuston in the Yellow Sea of China.展开更多
Grassland devoted about 90% of agricultural Irish land. Anion retention capacity of most agriculture soils is less than cation retention capacity, therefore chemical analyzed for anion （NO3 （nitrate）, CI- （chlorid...Grassland devoted about 90% of agricultural Irish land. Anion retention capacity of most agriculture soils is less than cation retention capacity, therefore chemical analyzed for anion （NO3 （nitrate）, CI- （chloride）, SO42 （sulphate） and HPO4 （biphosphate）） in different water flow types （overland flow, interflow and vertical flow or drainage） samples. In this work, simple equipment was used to operationally distinguish between overland flow and interflow while vertical flow collected in different depths using soil water sampler equipments. Episodes of overland flow and interflow occurred even though the site is located in the lowest rainfall in Ireland and on well-drained soil. Samples of different origin showed marked differences in their anion contents, while HPO4 concentrations were almost equal to zero in all water flow types, nitrate where relatively high in overland and interflow samples and was very high in drainage samples. No sampling was carried out below the root zone but it must be assumed that the high concentrations measured in drainage samples would constitute a threat to groundwater resources. When overland flow and interflow did occur, NO3 concentrations were usually close to or in excess of the maximum admissible concentrations for drinking water and it will be constituted a threat to inland surface water bodies.展开更多
The architectural patterns of sedimentary succession are diverse in different depositionalbasins. The sedimentary architecture and geological condition of such basins asepicontinental sea, intraplate limnic basins, et...The architectural patterns of sedimentary succession are diverse in different depositionalbasins. The sedimentary architecture and geological condition of such basins asepicontinental sea, intraplate limnic basins, etc., differ cIearly from those of continentalmargin basin. Extension, complement and perfection of sequence stratigraphic models are needed in the studies of various depositional basins based on the classical sequence model. This paper, for this reason,expounds the thought, principles of sequence division, methodology and technology of the study of sequence stratigraphy in epicontinental and limnic basins.展开更多
In the present work, uranium concentrations were measured in twenty surface soil samples forselected regions in AI-Anbargovernorate using fission track etch technique, CR-39 track detector was used for registration of...In the present work, uranium concentrations were measured in twenty surface soil samples forselected regions in AI-Anbargovernorate using fission track etch technique, CR-39 track detector was used for registration of induced fission tracks. The results show that, the uranium concentrations ranged from 4.61 ppm, 56.93 Bq/kg （AL-Falluja AL-Jolan District） to 1.81 ppm 22.35 Bq/kg （Anah）with an average 3.42±0.79 ppm, 41.76±9.04 Bq/kg. The present results have shown that the uranium concentrations in the studied surface soil samples were less than the allowed limit 11.7 ppm recommended by UNSCEAR, 1993.展开更多
In order to determine the environmental quality condition of reclaimed soils inHuainan mining area, soil samples were collected from three representative mines, suchas Panyi Mine, Xinzhuangzi Mine and Datong Mine.The ...In order to determine the environmental quality condition of reclaimed soils inHuainan mining area, soil samples were collected from three representative mines, suchas Panyi Mine, Xinzhuangzi Mine and Datong Mine.The total concentration of Cd, Hg, Cu,Pb and As in the samples were analyzed.The potential ecological risk was used to evaluatethe heavy metals pollution.The investigation reveals that the reclaimed soils are contaminatedto a certain degree and the trace elements in coal gangue transferred to thesurface soil.The order of potential ecological risk is Cd>Hg>Cu>Pb>As; the pollution degreeof each sampling site is arranged in the following order: Xinzhuangzi Mine>DatongMine>Panyi Mine, and the multiform of heavy metals of potential ecological risk index is at357.35～484.62.展开更多
基金supported by the National Key Technology Research and Development Program (Nos.2011 BAD13B02, 2010BAC68B01)the National High Technology Research and Development Program of China (863 program, No.2012AA10A412)+1 种基金the Science and Technology Development Projects Funds of Shinan District in Qingdao, Shandong Province (2011-5-023-QT)the National Science Foundation of China (No.41106145)
文摘This study investigated the bacterial diversity of gut content of sea cucumber （Apostichopus japonicus） and its habitat surface sediment in a bottom enhancement area using PCR-based denaturing gradient gel electrophoresis （DGGE） technique. Bacte- rial diversity evaluation showed that the value of the Shannon-Wiener index of gut content in different intestinal segments of A. ja-ponicus varied between 2.88 and 3.00, lower than that of the surrounding sediment （3.23）. Phyl0genetic analysis showed that bacte- rial phylotypes in gut content and the surrounding sediment ofA. japonicus were closely related to Proteobacteria including3,-, ct-, 8- and e-proteobacteria, Bacteroidetes, Firmicute, and Actinobacteria, of which 7-proteobacteria were predominant. These results sug- gested that the sea cucumber A. japonicus was capable of feeding selectively, and PCR-DGGE was applicable for characterizing the bacterial community composition in gut content and the surrounding sediment of sea cucumber. Further investigation targeting longer 16S rDNA gene fragments and/or functional genes was recommended for obtaining more information of the diversity and function of bacterial community in the gut content of sea cucumber.
基金supported by the Natural Science Foundation of China (No. 41406129)the National Key Research Program (Grant 2016YFA0601400)the State Oceanic Administration of China(Grant GASI-03-01-02-05)
文摘The surface microlayer(SML)is a thin biogenic film found between the marine environment and troposphere that comprises a unique marine ecosystem with its own physicochemical and microbiological compositions.In this study,we compared the bacterial cell abundance and community structure of the SML and subsurface waters(0.50m depth)using traditional culture and flow cytometry methods.Overall,53strains were screened and sequenced.In addition,DNA was extracted from the SML and corresponding subsurface water and used for bacterial community analysis by denaturing gradient gel electrophoresis(DGGE).The results revealed that the SML had a distinct microbial community structure from the corresponding subsurface water.This is the first compresensively detailed molecular biological analysis of the bacterioneuston in the Yellow Sea of China.
文摘Grassland devoted about 90% of agricultural Irish land. Anion retention capacity of most agriculture soils is less than cation retention capacity, therefore chemical analyzed for anion （NO3 （nitrate）, CI- （chloride）, SO42 （sulphate） and HPO4 （biphosphate）） in different water flow types （overland flow, interflow and vertical flow or drainage） samples. In this work, simple equipment was used to operationally distinguish between overland flow and interflow while vertical flow collected in different depths using soil water sampler equipments. Episodes of overland flow and interflow occurred even though the site is located in the lowest rainfall in Ireland and on well-drained soil. Samples of different origin showed marked differences in their anion contents, while HPO4 concentrations were almost equal to zero in all water flow types, nitrate where relatively high in overland and interflow samples and was very high in drainage samples. No sampling was carried out below the root zone but it must be assumed that the high concentrations measured in drainage samples would constitute a threat to groundwater resources. When overland flow and interflow did occur, NO3 concentrations were usually close to or in excess of the maximum admissible concentrations for drinking water and it will be constituted a threat to inland surface water bodies.
文摘The architectural patterns of sedimentary succession are diverse in different depositionalbasins. The sedimentary architecture and geological condition of such basins asepicontinental sea, intraplate limnic basins, etc., differ cIearly from those of continentalmargin basin. Extension, complement and perfection of sequence stratigraphic models are needed in the studies of various depositional basins based on the classical sequence model. This paper, for this reason,expounds the thought, principles of sequence division, methodology and technology of the study of sequence stratigraphy in epicontinental and limnic basins.
文摘In the present work, uranium concentrations were measured in twenty surface soil samples forselected regions in AI-Anbargovernorate using fission track etch technique, CR-39 track detector was used for registration of induced fission tracks. The results show that, the uranium concentrations ranged from 4.61 ppm, 56.93 Bq/kg （AL-Falluja AL-Jolan District） to 1.81 ppm 22.35 Bq/kg （Anah）with an average 3.42±0.79 ppm, 41.76±9.04 Bq/kg. The present results have shown that the uranium concentrations in the studied surface soil samples were less than the allowed limit 11.7 ppm recommended by UNSCEAR, 1993.
基金Supported by the Natural Science Foundation of China of Anhui Education to Research(KJ2009A088)
文摘In order to determine the environmental quality condition of reclaimed soils inHuainan mining area, soil samples were collected from three representative mines, suchas Panyi Mine, Xinzhuangzi Mine and Datong Mine.The total concentration of Cd, Hg, Cu,Pb and As in the samples were analyzed.The potential ecological risk was used to evaluatethe heavy metals pollution.The investigation reveals that the reclaimed soils are contaminatedto a certain degree and the trace elements in coal gangue transferred to thesurface soil.The order of potential ecological risk is Cd>Hg>Cu>Pb>As; the pollution degreeof each sampling site is arranged in the following order: Xinzhuangzi Mine>DatongMine>Panyi Mine, and the multiform of heavy metals of potential ecological risk index is at357.35～484.62.
