To improve image processing speed and detection precision of a surface detection system on a strip surface,based on the analysis of the characteristics of image data and image processing in detection system on the str...To improve image processing speed and detection precision of a surface detection system on a strip surface,based on the analysis of the characteristics of image data and image processing in detection system on the strip surface,the design of parallel image processing system and the methods of algorithm implementation have been studied. By using field programmable gate array(FPGA) as hardware platform of implementation and considering the characteristic of detection system on the strip surface,a parallel image processing system implemented by using multi IP kernel is designed. According to different computing tasks and the load balancing capability of parallel processing system,the system could set different calculating numbers of nodes to meet the system's demand and save the hardware cost.展开更多
This study presents a numerical method for optimizing hull form in calm water with respect to total drag which contains a viscous drag and a wave drag. The ITTC 1957 model-ship correlation line was used to predict fri...This study presents a numerical method for optimizing hull form in calm water with respect to total drag which contains a viscous drag and a wave drag. The ITTC 1957 model-ship correlation line was used to predict frictional drag and the corrected linearized thin-ship theory was employed to estimate the wave drag The evolution strategy (ES) which is a member of the evolutionary algorithms (EAs) family obtains an optimum hull form by considering some design constraints. Standard Wigley hull is considered as an initial hull in optimization procedures for two test cases and new hull forms were achieved at Froude numbers 0.24, 0.316 and 0.408. In one case the ES technique was ran for the initial hull form, where the main dimensions were fixed and the only variables were the hull offsets. In the other case in addition to hull offsets, the raain dimensions were considered as variables that are optimized simultaneously. The numerical results of optimization procedure demonstrate that the optimized hull forms yield a reduction in total drag.展开更多
AIM:To identify the novel methylation-silenced gene pentraxin 3(PTX3) in esophageal squamous cell carcinoma(ESCC).METHODS:PTX3 mRNA expression was examined in six human ESCC cell lines,one human immortalized normal es...AIM:To identify the novel methylation-silenced gene pentraxin 3(PTX3) in esophageal squamous cell carcinoma(ESCC).METHODS:PTX3 mRNA expression was examined in six human ESCC cell lines,one human immortalized normal esophageal epithelial cell line,primary ESCC tumor tissue,and paired adjacent nontumor tissue using reverse transcription polymerase chain reaction(RTPCR).Semi-quantitative immunohistochemistry was used to examine cellular localisation and protein levels.Methylation specific PCR and bisulphite genomic sequencing were employed to investigate the methylation of the candidate gene.RESULTS:In the majority of ESCC cell lines,we found that PTX3 expression was down-regulated due to gene promoter hypermethylation,which was further confirmed by bisulphite genomic sequencing.Demethylation treatment with 5-aza-2'-deoxycytidine restored PTX3 mRNA expression in ESCC cell lines.Methylation was more common in tumor tissues(85%) than in adjacent nontumor tissues(25%)(P < 0.01).CONCLUSION:PTX3 is down-regulated through promoter hypermethylation in ESCC,and could potentially serve as a biomarker of ESCC.展开更多
A superoxide dismutase was purified from Enteromorpha linza using a simple and safe procedure, which comprised phosphate buffer extraction, ammonium sulphate precipitation, ion exchange chromatography on Q-sepharose c...A superoxide dismutase was purified from Enteromorpha linza using a simple and safe procedure, which comprised phosphate buffer extraction, ammonium sulphate precipitation, ion exchange chromatography on Q-sepharose column, and gel filtration chromatography on Superdex 200 10/300GL. The E. linza superoxide dismutase (E/SOD) was purified 103.6-fold, and a yield of 19.1% and a specific activity of 1 750 U/rag protein were obtained. The SDS-PAGE exhibited E/SOD a single band near 23 kDa and the gel filtration study showed E/SOD's molecular weight is near 46 kDa in nondenatured condition, indicating it's a homodimeric protein. E/SOD is an iron-cofactored superoxide dismutase (Fe-SOD) because it was inhibited by hydrogen peroxide, insensitive to potassium cyanide. The optimal temperature for its maximal enzyme activity was 35℃, and it still had 29.8% relative activity at 0℃, then E/SOD can be classified as a cold-adapted enzyme. E/SOD was stable when temperature was below 40℃ or the pH was within the range of 5 10. The first 11 N-terminal amino acids orE/SOD were ALELKAPPYEL, comparison of its N-terminal sequence with other Fe-SOD N-terminal sequences at the same position suggests it is possibly a chloroplastic Fe-SOD.展开更多
Translation involves not only formal transformation of two languages concerned, but also conversion of thought patterns. The oriental thought pattern is considered "concrete", "man-centered", and "ordinal"; whil...Translation involves not only formal transformation of two languages concerned, but also conversion of thought patterns. The oriental thought pattern is considered "concrete", "man-centered", and "ordinal"; while the western one is believed to be "abstract", "object-centered", and "conversed". These are the decisive factors for the existence of the differences in language expressing. It is of great importance to study these differences and make some adjustments in terms of syntactic structure, semantic focus, and time order so as to achieve a natural rending.展开更多
For an entire function represented by a generalized dirichlet series, we define its maximal term, maximal modulus, order and type. We use the classical methods to study the relation between order, type and coeFFIcient...For an entire function represented by a generalized dirichlet series, we define its maximal term, maximal modulus, order and type. We use the classical methods to study the relation between order, type and coeFFIcients, exponents, which improve and generalize some results of the dirichlet series with real exponents.展开更多
Article 4 of the Labor Contract Law lays down the right of the Staff and Workers' Representative Congress (SWRC) to deliberate on the formulation of (intra-enterprise) work regulations, but this has become a "s...Article 4 of the Labor Contract Law lays down the right of the Staff and Workers' Representative Congress (SWRC) to deliberate on the formulation of (intra-enterprise) work regulations, but this has become a "soft" law in judicial practice. The judicial criteria for judging the validity of work regulations are in essence determined by the judge's judgment on their reasonableness. As an important embodiment of Chinese politics, economics and culture, the transformation of the SWRC that accompanied the market economy has not negated its value as an indigenous traditional resource. The SWRC does not just enjoy deliberative rights in the formation of regulations, as clearly specified in constitutional law, but also has rights under the law in local legislation and practice. Hence the system of work regulations is neither a unilateral decision on the part of management nor a contract, but rather an autonomous norm developed through the SWRC mechanism. Given the mandatory nature of Article 4 of the Labor Contract Law, regulations will only be valid after they havegone through a democratic process. The further development of the theory of normative system formation should endow the SWRC with greater rights and integrate it smoothly with the collective contract system to standardize collective labor relations.展开更多
An accurate frequency response characterization method for photoreceivers with optical heterodyne technique is presented in this paper.The characterization is implemented with two single-mode tunable lasers operating ...An accurate frequency response characterization method for photoreceivers with optical heterodyne technique is presented in this paper.The characterization is implemented with two single-mode tunable lasers operating near the wavelength of 1.55 μm.The errors introduced by extra fixtures as well as laser output fluctuations are considered and calibrated simultaneously.Compared with previous works,the proposed calibration procedures are more complete.Experimental results indicate that the significant improvement in measurement precision has been achieved with the proposed method in the frequency range from 0 to 30 GHz,which proves the proposed frequency response characterization method to be feasible and reliable.展开更多
Room-temperature ferromagnetism with a Curie temperature higher than 380 K was studied in GaN: Mn thin films grown by metal-organic chemical vapor deposition. By etching artificial microstructures on the GaN: Mn layer...Room-temperature ferromagnetism with a Curie temperature higher than 380 K was studied in GaN: Mn thin films grown by metal-organic chemical vapor deposition. By etching artificial microstructures on the GaN: Mn layer,strong magnetic responses were observed in the magnetic force microscopy (MFM) measurement,which revealed that the films were independent of dopant particles and clusters. Numerical simulation on the data of atomic force microscope (AFM) and MFM measurements covering the whole microstructure validated the formation of long range magnetic order. This result excluded a variety of controversial origins of room-temperature ferromagnetism in the GaN: Mn and gave a strong evidence of our GaN: Mn as the intrinsic diluted magnetic semiconductor (DMS). The forwarded method for accurate characterization of long range magnetic order could be applied to a wide range of DMS and diluted magnetic oxide (DMO) systems.展开更多
High-throughput pyrosequencing,carried out in millions of picoliter-sized reactors on a fiber-optic slide,is known for its longer read length.However,both optical crosstalk(which reduces the signal-to-noise ratio of C...High-throughput pyrosequencing,carried out in millions of picoliter-sized reactors on a fiber-optic slide,is known for its longer read length.However,both optical crosstalk(which reduces the signal-to-noise ratio of CCD images)and chemical retention adversely affect the accuracy of chemiluminescence determination,and ultimately decrease the read length and the accuracy of pyrosequencing results.In this study,both titanium and oxidized aluminum films were deposited on the side walls and upper faces of micro-reactor slides to enhance optical isolation;the films reduced the inter-well crosstalk by one order of magnitude.Subsequently,chemical retention was shown to be caused by the lower diffusion coefficient of the side walls of the picolitersized reactors because of surface roughness and random pores.Optically isolated fiber-optic slides over-coated with silicon oxide showed smoother surface morphology,resulting in little chemical retention;this was further confirmed with theoretical calculations.Picoliter-sized micro-reactors coated with titanium-silicon oxide films showed the least inter-well optical crosstalk and chemical retention;these properties are expected to greatly improve the high-throughput pyrosequencing performance.展开更多
基金The 111 project(B07018) Supported by Program for Changjiang Scholars and Innovative Research Teamin University(IRT0423)
文摘To improve image processing speed and detection precision of a surface detection system on a strip surface,based on the analysis of the characteristics of image data and image processing in detection system on the strip surface,the design of parallel image processing system and the methods of algorithm implementation have been studied. By using field programmable gate array(FPGA) as hardware platform of implementation and considering the characteristic of detection system on the strip surface,a parallel image processing system implemented by using multi IP kernel is designed. According to different computing tasks and the load balancing capability of parallel processing system,the system could set different calculating numbers of nodes to meet the system's demand and save the hardware cost.
基金marine research institute (MRC) of AUT for some financial support of this project
文摘This study presents a numerical method for optimizing hull form in calm water with respect to total drag which contains a viscous drag and a wave drag. The ITTC 1957 model-ship correlation line was used to predict frictional drag and the corrected linearized thin-ship theory was employed to estimate the wave drag The evolution strategy (ES) which is a member of the evolutionary algorithms (EAs) family obtains an optimum hull form by considering some design constraints. Standard Wigley hull is considered as an initial hull in optimization procedures for two test cases and new hull forms were achieved at Froude numbers 0.24, 0.316 and 0.408. In one case the ES technique was ran for the initial hull form, where the main dimensions were fixed and the only variables were the hull offsets. In the other case in addition to hull offsets, the raain dimensions were considered as variables that are optimized simultaneously. The numerical results of optimization procedure demonstrate that the optimized hull forms yield a reduction in total drag.
基金Supported by National High Technology Research and Development Program of China (863 Program),No. 2007AA02Z4Z4China Postdoctoral Science Foundation,No. 20090460394Beijing Municipal Natural Science Foundation,No. 7072022
文摘AIM:To identify the novel methylation-silenced gene pentraxin 3(PTX3) in esophageal squamous cell carcinoma(ESCC).METHODS:PTX3 mRNA expression was examined in six human ESCC cell lines,one human immortalized normal esophageal epithelial cell line,primary ESCC tumor tissue,and paired adjacent nontumor tissue using reverse transcription polymerase chain reaction(RTPCR).Semi-quantitative immunohistochemistry was used to examine cellular localisation and protein levels.Methylation specific PCR and bisulphite genomic sequencing were employed to investigate the methylation of the candidate gene.RESULTS:In the majority of ESCC cell lines,we found that PTX3 expression was down-regulated due to gene promoter hypermethylation,which was further confirmed by bisulphite genomic sequencing.Demethylation treatment with 5-aza-2'-deoxycytidine restored PTX3 mRNA expression in ESCC cell lines.Methylation was more common in tumor tissues(85%) than in adjacent nontumor tissues(25%)(P < 0.01).CONCLUSION:PTX3 is down-regulated through promoter hypermethylation in ESCC,and could potentially serve as a biomarker of ESCC.
基金Supported by the National Key Technology R&D Program of China(No.2012BAC07B03)
文摘A superoxide dismutase was purified from Enteromorpha linza using a simple and safe procedure, which comprised phosphate buffer extraction, ammonium sulphate precipitation, ion exchange chromatography on Q-sepharose column, and gel filtration chromatography on Superdex 200 10/300GL. The E. linza superoxide dismutase (E/SOD) was purified 103.6-fold, and a yield of 19.1% and a specific activity of 1 750 U/rag protein were obtained. The SDS-PAGE exhibited E/SOD a single band near 23 kDa and the gel filtration study showed E/SOD's molecular weight is near 46 kDa in nondenatured condition, indicating it's a homodimeric protein. E/SOD is an iron-cofactored superoxide dismutase (Fe-SOD) because it was inhibited by hydrogen peroxide, insensitive to potassium cyanide. The optimal temperature for its maximal enzyme activity was 35℃, and it still had 29.8% relative activity at 0℃, then E/SOD can be classified as a cold-adapted enzyme. E/SOD was stable when temperature was below 40℃ or the pH was within the range of 5 10. The first 11 N-terminal amino acids orE/SOD were ALELKAPPYEL, comparison of its N-terminal sequence with other Fe-SOD N-terminal sequences at the same position suggests it is possibly a chloroplastic Fe-SOD.
文摘Translation involves not only formal transformation of two languages concerned, but also conversion of thought patterns. The oriental thought pattern is considered "concrete", "man-centered", and "ordinal"; while the western one is believed to be "abstract", "object-centered", and "conversed". These are the decisive factors for the existence of the differences in language expressing. It is of great importance to study these differences and make some adjustments in terms of syntactic structure, semantic focus, and time order so as to achieve a natural rending.
基金the Natural Science Youth Foundation of Jiangxi Province (No.2007GQS0159)Research Plan Program of Education Bureau of Jiangxi Province (Nos.GJJ08161 GJJ09463)
文摘For an entire function represented by a generalized dirichlet series, we define its maximal term, maximal modulus, order and type. We use the classical methods to study the relation between order, type and coeFFIcients, exponents, which improve and generalize some results of the dirichlet series with real exponents.
文摘Article 4 of the Labor Contract Law lays down the right of the Staff and Workers' Representative Congress (SWRC) to deliberate on the formulation of (intra-enterprise) work regulations, but this has become a "soft" law in judicial practice. The judicial criteria for judging the validity of work regulations are in essence determined by the judge's judgment on their reasonableness. As an important embodiment of Chinese politics, economics and culture, the transformation of the SWRC that accompanied the market economy has not negated its value as an indigenous traditional resource. The SWRC does not just enjoy deliberative rights in the formation of regulations, as clearly specified in constitutional law, but also has rights under the law in local legislation and practice. Hence the system of work regulations is neither a unilateral decision on the part of management nor a contract, but rather an autonomous norm developed through the SWRC mechanism. Given the mandatory nature of Article 4 of the Labor Contract Law, regulations will only be valid after they havegone through a democratic process. The further development of the theory of normative system formation should endow the SWRC with greater rights and integrate it smoothly with the collective contract system to standardize collective labor relations.
文摘An accurate frequency response characterization method for photoreceivers with optical heterodyne technique is presented in this paper.The characterization is implemented with two single-mode tunable lasers operating near the wavelength of 1.55 μm.The errors introduced by extra fixtures as well as laser output fluctuations are considered and calibrated simultaneously.Compared with previous works,the proposed calibration procedures are more complete.Experimental results indicate that the significant improvement in measurement precision has been achieved with the proposed method in the frequency range from 0 to 30 GHz,which proves the proposed frequency response characterization method to be feasible and reliable.
基金supported by the National Natural Science Foundation of China (Grant Nos.60577030,60776041,60876035)the National Key Basic Research Special Foundation of China (Grant Nos.TG2007CB307004,2006CB921607)
文摘Room-temperature ferromagnetism with a Curie temperature higher than 380 K was studied in GaN: Mn thin films grown by metal-organic chemical vapor deposition. By etching artificial microstructures on the GaN: Mn layer,strong magnetic responses were observed in the magnetic force microscopy (MFM) measurement,which revealed that the films were independent of dopant particles and clusters. Numerical simulation on the data of atomic force microscope (AFM) and MFM measurements covering the whole microstructure validated the formation of long range magnetic order. This result excluded a variety of controversial origins of room-temperature ferromagnetism in the GaN: Mn and gave a strong evidence of our GaN: Mn as the intrinsic diluted magnetic semiconductor (DMS). The forwarded method for accurate characterization of long range magnetic order could be applied to a wide range of DMS and diluted magnetic oxide (DMO) systems.
基金supported by the Scientific Equipment Research Project of Chinese Academy of Sciences(YZ200823)
文摘High-throughput pyrosequencing,carried out in millions of picoliter-sized reactors on a fiber-optic slide,is known for its longer read length.However,both optical crosstalk(which reduces the signal-to-noise ratio of CCD images)and chemical retention adversely affect the accuracy of chemiluminescence determination,and ultimately decrease the read length and the accuracy of pyrosequencing results.In this study,both titanium and oxidized aluminum films were deposited on the side walls and upper faces of micro-reactor slides to enhance optical isolation;the films reduced the inter-well crosstalk by one order of magnitude.Subsequently,chemical retention was shown to be caused by the lower diffusion coefficient of the side walls of the picolitersized reactors because of surface roughness and random pores.Optically isolated fiber-optic slides over-coated with silicon oxide showed smoother surface morphology,resulting in little chemical retention;this was further confirmed with theoretical calculations.Picoliter-sized micro-reactors coated with titanium-silicon oxide films showed the least inter-well optical crosstalk and chemical retention;these properties are expected to greatly improve the high-throughput pyrosequencing performance.