Proteins of the DYRK(dual-specificity tyrosine-phosphorylation-regulated kinase) family are characterized by the presence of a conserved kinase domain and N-terminal DH box.DYRK2 is involved in regulating key developm...Proteins of the DYRK(dual-specificity tyrosine-phosphorylation-regulated kinase) family are characterized by the presence of a conserved kinase domain and N-terminal DH box.DYRK2 is involved in regulating key developmental and cellular processes,such as neurogenesis,cell proliferation,cytokinesis,and cellular differentiation.Herein,we report that the ortholog of DYRK2 found in zebrafish shares about 70% identity with that of human,mouse,and chick.RT-PCR showed that DYRK2 is expressed maternally and zygotically.In-situ hybridization results show that DYRK2 is expressed in somite cells that will develop into muscles.Our results provide preliminary evidence for investigating the in-vivo function of DYRK2 in zebrafish muscle development.展开更多
Currently available anti-HIV-1 drugs suppress viral replication and maintain viral levels below the detection threshold of most assays but do not eliminate cellular reservoirs. As a result, very low levels of circulat...Currently available anti-HIV-1 drugs suppress viral replication and maintain viral levels below the detection threshold of most assays but do not eliminate cellular reservoirs. As a result, very low levels of circulating virus can be detected in most people despite long-term treatment with potent anti-HIV drug combinations. Not surprisingly, viral levels rebound with discontinuation of treatment. New evidence indicates that there is a viral reservoir in bone marrow progenitor cells.展开更多
The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation an...The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified： mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom （RNV） was strongest in Tris-HCl buffer （50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaC1）. The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 μg/mL.展开更多
Nitrogen-doped carbon nanotubes (N-CNTs)/polyaniline (PANI) composites are developed as an electrode material for biosensors. The morphology, composition, and optical properties of the resulting products were characte...Nitrogen-doped carbon nanotubes (N-CNTs)/polyaniline (PANI) composites are developed as an electrode material for biosensors. The morphology, composition, and optical properties of the resulting products were characterized by transmission electron microscopy (TEM), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), and ultraviolet-visible absorption spectra (UV-vis). Furthermore, N-CNTs/PANI composite was immobilized on the surface of a glassy carbon electrode (GCE) and applied to construct a sensor. The obtained N-CNTs/PANI-modified GCE showed one pair of redox peaks and high catalytic activity for the oxidation of dopamine (DA) in a neutral environment. Differential pulse voltam-mograms results illustrate that the fabricated DA biosensor has high anti-interference ability towards ascorbic acid (AA). In addition, the fabricated biosensor showed superior performances with two wide linear ranges from 1 to 80 μM and from 1.5 to 3.5 mM and a low detection limit of 0.01 μM.展开更多
Using two-step growth method and buffer layer annealing treatment,the double heterojunction structures of In_(0.82)Ga_(0.18) As epilayer capped with In As_(0.6)P0.4 layer were prepared on In P substrate by low pressur...Using two-step growth method and buffer layer annealing treatment,the double heterojunction structures of In_(0.82)Ga_(0.18) As epilayer capped with In As_(0.6)P0.4 layer were prepared on In P substrate by low pressure metal organic chemical vapor deposition(LP-MOCVD).Based on the high quality In_(0.82)Ga_(0.18) As structures,the In_(0.82)Ga_(0.18) As PIN photodetector with cut-off wavelength of 2.56 μm at room temperature was fabricated by planar semiconductor technology,and the device performance was investigated in detail.The typical dark current at the reverse bias VR=10 m V and the resistance area product R0 A are 5.02 μA and 0.29 ?·cm2 at 296 K and 5.98 n A and 405.2 ?·cm2 at 116 K,respectively.The calculated peak detectivities of the In_(0.82)Ga_(0.18) As photodetector are 1.21×1010 cm·Hz1/2/W at 296 K and 4.39×1011 cm·Hz1/2/W at 116 K respectively,where the quantum efficiency η=0.7 at peak wavelength is supposed.The results show that the detection performance of In_(0.82)Ga_(0.18) As prepared by two-step growth method can be improved greatly.展开更多
基金Supported by the Open Research Fund Program of Key Laboratory of Marine Drugs(Ocean University of China),Grant of Ministry of Education to X.TAN,KLMD(OUC)200609the National Natural Science Foundation of China(No.30970423)
文摘Proteins of the DYRK(dual-specificity tyrosine-phosphorylation-regulated kinase) family are characterized by the presence of a conserved kinase domain and N-terminal DH box.DYRK2 is involved in regulating key developmental and cellular processes,such as neurogenesis,cell proliferation,cytokinesis,and cellular differentiation.Herein,we report that the ortholog of DYRK2 found in zebrafish shares about 70% identity with that of human,mouse,and chick.RT-PCR showed that DYRK2 is expressed maternally and zygotically.In-situ hybridization results show that DYRK2 is expressed in somite cells that will develop into muscles.Our results provide preliminary evidence for investigating the in-vivo function of DYRK2 in zebrafish muscle development.
文摘Currently available anti-HIV-1 drugs suppress viral replication and maintain viral levels below the detection threshold of most assays but do not eliminate cellular reservoirs. As a result, very low levels of circulating virus can be detected in most people despite long-term treatment with potent anti-HIV drug combinations. Not surprisingly, viral levels rebound with discontinuation of treatment. New evidence indicates that there is a viral reservoir in bone marrow progenitor cells.
基金Supported by Qingdao Science and Technology Project (No. 08-1-3-51- JCH)the Award Foundation of Scientific Research for Excellent Young and Middle-Age Scientist of Shandong Province (No.2006BS 07003)
文摘The present work is the first report of the biochemical characterization of the venom from nematocysts of the jellyfish Rhopilema esculentum Kishinouye. The nematocysts were isolated by autolysis and centrifugation and separated by flow cytometry. Four types of nematocysts were identified： mastigophores, euryteles, and atrichous and holotrichous isorhiza. SDS-PAGE and amino acid analyses demonstrated that most of the proteins in the nematocyst extract were between 10 kDa and 40 kDa, and that glutamic acid was the main amino acid. A hemolytic activity assay showed that the activity of the nematocyst venom （RNV） was strongest in Tris-HCl buffer （50 mmol/L, pH 7.8, 5% glycerol, 0.5 mmol/L EDTA, 0.1 mol/L NaC1）. The hemolytic activity was related to protein concentration and the HU50 against chicken erythrocytes was 0.91 μg/mL.
基金supported by the National Natural Science Foundation of China (20905038, 20903057, 20974046, 50803027, 20874048 & 20804020)the National Basic Research Program of China (2009CB930600)+1 种基金the Natural Science Foundation of Jiangsu Province (08KJB150011 & 09KJB150007)the Fok Ying-Tong Education Foundation under Grant 111051
文摘Nitrogen-doped carbon nanotubes (N-CNTs)/polyaniline (PANI) composites are developed as an electrode material for biosensors. The morphology, composition, and optical properties of the resulting products were characterized by transmission electron microscopy (TEM), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), and ultraviolet-visible absorption spectra (UV-vis). Furthermore, N-CNTs/PANI composite was immobilized on the surface of a glassy carbon electrode (GCE) and applied to construct a sensor. The obtained N-CNTs/PANI-modified GCE showed one pair of redox peaks and high catalytic activity for the oxidation of dopamine (DA) in a neutral environment. Differential pulse voltam-mograms results illustrate that the fabricated DA biosensor has high anti-interference ability towards ascorbic acid (AA). In addition, the fabricated biosensor showed superior performances with two wide linear ranges from 1 to 80 μM and from 1.5 to 3.5 mM and a low detection limit of 0.01 μM.
基金supported by the National Natural Science Foundation of China(Nos.11174224 and 11404246)the Natural Science Foundation of Shandong Province(Nos.BS2015DX015 and ZR2013FM001)+1 种基金the Science and Technology Development Program of Shandong Province(No.2013YD01016)the Higher School Science and Technology Program of Shandong Province(Nos.J13LJ54 and J15LJ54)
文摘Using two-step growth method and buffer layer annealing treatment,the double heterojunction structures of In_(0.82)Ga_(0.18) As epilayer capped with In As_(0.6)P0.4 layer were prepared on In P substrate by low pressure metal organic chemical vapor deposition(LP-MOCVD).Based on the high quality In_(0.82)Ga_(0.18) As structures,the In_(0.82)Ga_(0.18) As PIN photodetector with cut-off wavelength of 2.56 μm at room temperature was fabricated by planar semiconductor technology,and the device performance was investigated in detail.The typical dark current at the reverse bias VR=10 m V and the resistance area product R0 A are 5.02 μA and 0.29 ?·cm2 at 296 K and 5.98 n A and 405.2 ?·cm2 at 116 K,respectively.The calculated peak detectivities of the In_(0.82)Ga_(0.18) As photodetector are 1.21×1010 cm·Hz1/2/W at 296 K and 4.39×1011 cm·Hz1/2/W at 116 K respectively,where the quantum efficiency η=0.7 at peak wavelength is supposed.The results show that the detection performance of In_(0.82)Ga_(0.18) As prepared by two-step growth method can be improved greatly.
