Compatible and incompatible reactions in rice plants ( Oryza sativa L. cv. Shenxianggen No.4) were resulted from inoculation with two different virulent races of rice blast fungus ( Magnaporthe grisea (Hebert) B...Compatible and incompatible reactions in rice plants ( Oryza sativa L. cv. Shenxianggen No.4) were resulted from inoculation with two different virulent races of rice blast fungus ( Magnaporthe grisea (Hebert) Barr), and thus an effective infecting system was established between rice plants and the rice blast pathogen. Two cDNA clones that showed induced and temporal patterns in expression in the very early stage in response to infection of the fungus were obtained from the plants by use of differential display. Of the two cDNA clones, Fastresp_a was induced to express in both compatible and incompatible interactions although it was expressed earlier in the former reaction. The second one, Fastresp_b , was only expressed in incompatible interaction. Southern blot analysis of the rice genomic DNA indicated that both of the two clones were from genome of the plant. No significant homology to the two genes was found from the rice gene database. This suggested that they were novel genes in rice and may play important roles in rice resistant response to infection of rice blast fungus.展开更多
Based on the relationship between water environment system and human society, water environment carrying capacity （WECC） probes into supporting ability of complex water environment system to the human society. Recen...Based on the relationship between water environment system and human society, water environment carrying capacity （WECC） probes into supporting ability of complex water environment system to the human society. Recent years, due to the shortage of water resources and serious water pollution in several watersheds in China, the research of watershed water environment carrying capacity （WWECC） becomes very important. The conception, connotation and method of representation of WWECC are discussed deeply in this paper. It shows that WWECC is a kind of index that instructs whether the water environment system in watershed can continue to support the development of social economy and ecology, it is dimensionless number.展开更多
The wave diffraction and radiation around a floating body is considered within the framework of the linear potential theory in a fairly perfect fluid. The fluid domain extended infinitely in the horizontal directions ...The wave diffraction and radiation around a floating body is considered within the framework of the linear potential theory in a fairly perfect fluid. The fluid domain extended infinitely in the horizontal directions but is limited by the sea bed, the body hull, and the part of the free surface excluding the body waterplane, and is subdivided into two subdomains according to the body geometry. The two subdomains are connected by a control surface in fluid. In each subdomain, the velocity potential is described by using the usual boundary integral representation involving Green functions. The boundary integral equations are then established by satisfying the boundary conditions and the continuous condition of the potential and the normal derivation across the control surface. This multi-domain boundary element method （MDBEM） is particularly interesting for bodies with a hull form including moonpools to which the usual BEM presents singularities and slow convergence of numerical results. The application of the MDBEM to study the resonant motion of a water column in moonpools shows that the MDBEM provides an efficient and reliable prediction method.展开更多
Rice stripe virus (RSV) infects rice and is transmitted in a propagative manner by the small brown planthopper. How RSV enters an insect cell to initiate the infection cycle is poorly understood. Sequence analysis rev...Rice stripe virus (RSV) infects rice and is transmitted in a propagative manner by the small brown planthopper. How RSV enters an insect cell to initiate the infection cycle is poorly understood. Sequence analysis revealed that the RSV NSvc2 protein was similar to the membrane glycoproteins of several members in the family Bunyaviridae and might induce cell membrane fusion. To conveniently study the membrane fusion activity of NSvc2, we constructed cell surface display vectors for expressing Nsvc2 on the insect cell surface as the membrane glycoproteins of the enveloped viruses. Our results showed that NSvc2 was successfully expressed and displayed on the surface of insect Sf9 cells. When induced by low pH, the membrane fusion was not observed in the cells that expressed NSvc2. Additionally, the membrane fusion was also not detected when co-expressing Nsvc2 and the viral capsid protein on insect cell surface. Thus, RSV NSvc2 is probably different from the phlebovirus counterparts, which could suggest different functions. RSV might enter insect cells other than by fusion with plasma or endosome membrane.展开更多
文摘Compatible and incompatible reactions in rice plants ( Oryza sativa L. cv. Shenxianggen No.4) were resulted from inoculation with two different virulent races of rice blast fungus ( Magnaporthe grisea (Hebert) Barr), and thus an effective infecting system was established between rice plants and the rice blast pathogen. Two cDNA clones that showed induced and temporal patterns in expression in the very early stage in response to infection of the fungus were obtained from the plants by use of differential display. Of the two cDNA clones, Fastresp_a was induced to express in both compatible and incompatible interactions although it was expressed earlier in the former reaction. The second one, Fastresp_b , was only expressed in incompatible interaction. Southern blot analysis of the rice genomic DNA indicated that both of the two clones were from genome of the plant. No significant homology to the two genes was found from the rice gene database. This suggested that they were novel genes in rice and may play important roles in rice resistant response to infection of rice blast fungus.
文摘Based on the relationship between water environment system and human society, water environment carrying capacity （WECC） probes into supporting ability of complex water environment system to the human society. Recent years, due to the shortage of water resources and serious water pollution in several watersheds in China, the research of watershed water environment carrying capacity （WWECC） becomes very important. The conception, connotation and method of representation of WWECC are discussed deeply in this paper. It shows that WWECC is a kind of index that instructs whether the water environment system in watershed can continue to support the development of social economy and ecology, it is dimensionless number.
文摘The wave diffraction and radiation around a floating body is considered within the framework of the linear potential theory in a fairly perfect fluid. The fluid domain extended infinitely in the horizontal directions but is limited by the sea bed, the body hull, and the part of the free surface excluding the body waterplane, and is subdivided into two subdomains according to the body geometry. The two subdomains are connected by a control surface in fluid. In each subdomain, the velocity potential is described by using the usual boundary integral representation involving Green functions. The boundary integral equations are then established by satisfying the boundary conditions and the continuous condition of the potential and the normal derivation across the control surface. This multi-domain boundary element method （MDBEM） is particularly interesting for bodies with a hull form including moonpools to which the usual BEM presents singularities and slow convergence of numerical results. The application of the MDBEM to study the resonant motion of a water column in moonpools shows that the MDBEM provides an efficient and reliable prediction method.
基金Natural Science Foundation of China Grants (30970138)the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Rice stripe virus (RSV) infects rice and is transmitted in a propagative manner by the small brown planthopper. How RSV enters an insect cell to initiate the infection cycle is poorly understood. Sequence analysis revealed that the RSV NSvc2 protein was similar to the membrane glycoproteins of several members in the family Bunyaviridae and might induce cell membrane fusion. To conveniently study the membrane fusion activity of NSvc2, we constructed cell surface display vectors for expressing Nsvc2 on the insect cell surface as the membrane glycoproteins of the enveloped viruses. Our results showed that NSvc2 was successfully expressed and displayed on the surface of insect Sf9 cells. When induced by low pH, the membrane fusion was not observed in the cells that expressed NSvc2. Additionally, the membrane fusion was also not detected when co-expressing Nsvc2 and the viral capsid protein on insect cell surface. Thus, RSV NSvc2 is probably different from the phlebovirus counterparts, which could suggest different functions. RSV might enter insect cells other than by fusion with plasma or endosome membrane.
