Blood clam, Tegillarca granosa, is an important shellfish in Chinese mariculture industry. Investigative research in this species, such as genetic linkage mapping, requires a large panel of molecular markers. In prese...Blood clam, Tegillarca granosa, is an important shellfish in Chinese mariculture industry. Investigative research in this species, such as genetic linkage mapping, requires a large panel of molecular markers. In present study, a total of 89 polymorphic microsatellite markers were developed in T. granosa using the sequence database of Life Sciences Technology 454 next generation sequencing technology. All 89 loci were characterized in 20 individual clams from a natural population inhabiting Yueqing Gulf, Zhejiang Province, China. The number of alleles per polymorphic locus varied between 2 and 15, while the observed heterozygosity, expected heterozygosity and polymorphic information content varied between 0.000 and 1.000, 0.102 and 0.921, and 0.048 and 0.886, respectively. Of the 89 loci identified, 32 loci deviated significantly from Hardy-Weinberg equilibrium following Bonferroni correction. Thirty nine markers, which were shown to be polymorphic in a full-sibling family, were tested in Mendelian segregations. As expected, 32 loci were co-dominantly segregated in a Mendelian fashion. These novel developed microsatellite markers represent useful research tools for investigation of population genetic structure and genetic diversity in this species.展开更多
The development of expressed sequence tags(ESTs) from pea has provided a useful source for mining novel simple sequence repeat(SSR) markers.In the present research,in order to find EST-derived SSR markers,18 552 pea E...The development of expressed sequence tags(ESTs) from pea has provided a useful source for mining novel simple sequence repeat(SSR) markers.In the present research,in order to find EST-derived SSR markers,18 552 pea ESTs from the National Center for Biotechnology Information(NCBI) database were downloaded and assembled into 10 086 unigenes.A total of 586 microsatellites in 530 unigenes were identified,indicating that merely 5.25% of sequences contained SSRs.The most abundant SSRs within pea were tri-nucleotide repeat motifs,and among all the tri-nucleotide repeats,the motif GAA was the most abundant type.In total,49 SSRs were used for primer design.EST-SSR loci were subsequently screened on 10 widely adapted varieties in China.Of these,nine loci showed polymorphic profiles that revealed two to three alleles per locus.The polymorphism information content value ranged from 0.18 to 0.58 with an average of 0.41.Furthermore,transferable analysis revealed that some of these loci showed transferability to faba bean.Because of their polymorphism and transferability,these nine novel EST-SSRs will be valuable tools for marker-assisted breeding and comparative mapping of pea in the future.展开更多
基金supported by the National High Technology Research and Development Program of China (863 Program) (No. 2012AA10A410)the National Project for Agricultural Technology System (No. CARS-48)+1 种基金National Infrastructure of Fishery Germplasm Resources of China (No. 2015DKA30470)Zhejiang Major Program of Science and Technology (No. 2012C12907-4)
文摘Blood clam, Tegillarca granosa, is an important shellfish in Chinese mariculture industry. Investigative research in this species, such as genetic linkage mapping, requires a large panel of molecular markers. In present study, a total of 89 polymorphic microsatellite markers were developed in T. granosa using the sequence database of Life Sciences Technology 454 next generation sequencing technology. All 89 loci were characterized in 20 individual clams from a natural population inhabiting Yueqing Gulf, Zhejiang Province, China. The number of alleles per polymorphic locus varied between 2 and 15, while the observed heterozygosity, expected heterozygosity and polymorphic information content varied between 0.000 and 1.000, 0.102 and 0.921, and 0.048 and 0.886, respectively. Of the 89 loci identified, 32 loci deviated significantly from Hardy-Weinberg equilibrium following Bonferroni correction. Thirty nine markers, which were shown to be polymorphic in a full-sibling family, were tested in Mendelian segregations. As expected, 32 loci were co-dominantly segregated in a Mendelian fashion. These novel developed microsatellite markers represent useful research tools for investigation of population genetic structure and genetic diversity in this species.
基金supported by the Zhejiang Provincial Science and Technol-ogy Program (No. 2007C32013)the Zhejiang Provincial Natural Science Foundation (No. Y3090660) of China
文摘The development of expressed sequence tags(ESTs) from pea has provided a useful source for mining novel simple sequence repeat(SSR) markers.In the present research,in order to find EST-derived SSR markers,18 552 pea ESTs from the National Center for Biotechnology Information(NCBI) database were downloaded and assembled into 10 086 unigenes.A total of 586 microsatellites in 530 unigenes were identified,indicating that merely 5.25% of sequences contained SSRs.The most abundant SSRs within pea were tri-nucleotide repeat motifs,and among all the tri-nucleotide repeats,the motif GAA was the most abundant type.In total,49 SSRs were used for primer design.EST-SSR loci were subsequently screened on 10 widely adapted varieties in China.Of these,nine loci showed polymorphic profiles that revealed two to three alleles per locus.The polymorphism information content value ranged from 0.18 to 0.58 with an average of 0.41.Furthermore,transferable analysis revealed that some of these loci showed transferability to faba bean.Because of their polymorphism and transferability,these nine novel EST-SSRs will be valuable tools for marker-assisted breeding and comparative mapping of pea in the future.
