[Objective] This study aimed to explore the proteins related to pistillate flower development in different mulberry cultivars. [Method] The total proteins of the pistillate flowers of two mulberry cultivars Dal0 （Mor...[Objective] This study aimed to explore the proteins related to pistillate flower development in different mulberry cultivars. [Method] The total proteins of the pistillate flowers of two mulberry cultivars Dal0 （Morus atropurpurea Roxb.） and SG01 （Morus muIticaulis Perr.） were extracted, separated and detected through two- dimensional electrophoresis （2-DE） and mass spectrometry. [Result] There was sig- nificant difference in the expression of proteins from the pistillate flowers of different mulberry cultivars. From the 2-DE images of Dal0 and SG01, 445_＋17 and 425_＋12 protein spots were respectively detected. The expression levels of 75 protein spots differed significantly. Thirteen spots those were expressed at high levels and well separated were analyzed by mass spectrometry, and nine of them were identified successfully. The nine proteins are involved in the glycometabolism, protein and amino acid metabolism and defense responses during the development of mulberry pistillate flower after they were pollinated. [Conclusion] The findings will provide reference for further study on the molecular mechanism of mulberry pistillate flower de- velopment.展开更多
Aluminum (Al) toxicity is the major factor limiting crop productivity in acid soils. In this study, a recombinant inbreed line (RIL) population derived from a cross between an Al sensitive lowland indica rice variety...Aluminum (Al) toxicity is the major factor limiting crop productivity in acid soils. In this study, a recombinant inbreed line (RIL) population derived from a cross between an Al sensitive lowland indica rice variety IR1552 and an Al tolerant upland japonica rice variety Azucena, was used for mapping quantitative trait loci (QTLs) for Al tolerance. Three QTLs for relative root length (RRL) were detected on chromosome 1, 9, 12, respectively, and 1 QTL for root length under Al stress is identical on chromosome 1 after one week and two weeks stress. Comparison of QTLs on chromosome 1 from different studies indicated an identical interval between C86 and RZ801 with gene(s) for Al tolerance. This interval provides an important start point for isolating genes responsible for Al tolerance and understanding the genetic nature of Al tolerance in rice. Four Al induced ESTs located in this interval were screened by reverse Northern analysis and confirmed by Northern analysis. They would be candidate genes for the QTL.展开更多
Nitrogen (N) is an essential macronutrient and an important factor limiting agricultural productivity. N deficient or excess conditions often occur during the cotton growth season and incorrect N application may affec...Nitrogen (N) is an essential macronutrient and an important factor limiting agricultural productivity. N deficient or excess conditions often occur during the cotton growth season and incorrect N application may affect cotton fiber yield and quality. Here, the influence of N stress on the cotton fiber proteome was investigated by two-dimensional gel electrophoresis and mass spectrometry. The results indicated that N application rate affects nitrogen accumulation in fiber cells and fiber length. The proteins differentially expressed during N stress were mainly related to plant carbohydrate metabolism, cell wall component synthesis and transportation, protein/amino acid metabolism, antioxidation and hormone metabolism. The most abundant proteins were C metabolism-related. Ten days post anthesis is a critical time for fiber cells to perceive environmental stress and most proteins were suppressed in both N deficient and N excess conditions at this sampling stage. However, several N metabolism proteins were increased to enhance N stress tolerance. Excess N may suppress carbohydrate/energy metabolism in early fiber development much like N deficiency. These results have identified some interesting proteins that can be further analyzed to elucidate the molecular mechanisms of N tolerance.展开更多
Quantum correlations in a family of states comprising any mixture of a pair of arbitrary bi-qubit product pure states are studied by employing geometric discord [Phys. Rev. Lett. 105(2010) 190502] as the quantifier. F...Quantum correlations in a family of states comprising any mixture of a pair of arbitrary bi-qubit product pure states are studied by employing geometric discord [Phys. Rev. Lett. 105(2010) 190502] as the quantifier. First, the inherent symmetry in the family of states about local unitary transformations is revealed. Then, the analytic expression of geometric discords in the states is worked out. Some concrete discussions and analyses on the captured geometric discords are made so that their distinct features are exposed. It is found that, the more averagely the two bi-qubit product states are mixed, the bigger geometric discord the mixed state owns. Moreover, the monotonic relationships of geometric discord with different parameters are revealed.展开更多
基金Supported by National Natural Science Foundation of China(31072087)~~
文摘[Objective] This study aimed to explore the proteins related to pistillate flower development in different mulberry cultivars. [Method] The total proteins of the pistillate flowers of two mulberry cultivars Dal0 （Morus atropurpurea Roxb.） and SG01 （Morus muIticaulis Perr.） were extracted, separated and detected through two- dimensional electrophoresis （2-DE） and mass spectrometry. [Result] There was sig- nificant difference in the expression of proteins from the pistillate flowers of different mulberry cultivars. From the 2-DE images of Dal0 and SG01, 445_＋17 and 425_＋12 protein spots were respectively detected. The expression levels of 75 protein spots differed significantly. Thirteen spots those were expressed at high levels and well separated were analyzed by mass spectrometry, and nine of them were identified successfully. The nine proteins are involved in the glycometabolism, protein and amino acid metabolism and defense responses during the development of mulberry pistillate flower after they were pollinated. [Conclusion] The findings will provide reference for further study on the molecular mechanism of mulberry pistillate flower de- velopment.
基金Project (No. 30070070) supported by the National NaturalScience Foundation of China
文摘Aluminum (Al) toxicity is the major factor limiting crop productivity in acid soils. In this study, a recombinant inbreed line (RIL) population derived from a cross between an Al sensitive lowland indica rice variety IR1552 and an Al tolerant upland japonica rice variety Azucena, was used for mapping quantitative trait loci (QTLs) for Al tolerance. Three QTLs for relative root length (RRL) were detected on chromosome 1, 9, 12, respectively, and 1 QTL for root length under Al stress is identical on chromosome 1 after one week and two weeks stress. Comparison of QTLs on chromosome 1 from different studies indicated an identical interval between C86 and RZ801 with gene(s) for Al tolerance. This interval provides an important start point for isolating genes responsible for Al tolerance and understanding the genetic nature of Al tolerance in rice. Four Al induced ESTs located in this interval were screened by reverse Northern analysis and confirmed by Northern analysis. They would be candidate genes for the QTL.
基金supported by the National Natural Science Foundation of China (Grant No. 30971735)the Specialized Research Funds for the Doctoral Program of Higher Education (Grant No.200803070017)
文摘Nitrogen (N) is an essential macronutrient and an important factor limiting agricultural productivity. N deficient or excess conditions often occur during the cotton growth season and incorrect N application may affect cotton fiber yield and quality. Here, the influence of N stress on the cotton fiber proteome was investigated by two-dimensional gel electrophoresis and mass spectrometry. The results indicated that N application rate affects nitrogen accumulation in fiber cells and fiber length. The proteins differentially expressed during N stress were mainly related to plant carbohydrate metabolism, cell wall component synthesis and transportation, protein/amino acid metabolism, antioxidation and hormone metabolism. The most abundant proteins were C metabolism-related. Ten days post anthesis is a critical time for fiber cells to perceive environmental stress and most proteins were suppressed in both N deficient and N excess conditions at this sampling stage. However, several N metabolism proteins were increased to enhance N stress tolerance. Excess N may suppress carbohydrate/energy metabolism in early fiber development much like N deficiency. These results have identified some interesting proteins that can be further analyzed to elucidate the molecular mechanisms of N tolerance.
基金Supported by the National Natural Science Foundation of China(NNSFC)under Grant Nos.11375011 and 11372122the Natural Science Foundation of Anhui Province under Grant No.1408085MA12the 211 Project of Anhui University
文摘Quantum correlations in a family of states comprising any mixture of a pair of arbitrary bi-qubit product pure states are studied by employing geometric discord [Phys. Rev. Lett. 105(2010) 190502] as the quantifier. First, the inherent symmetry in the family of states about local unitary transformations is revealed. Then, the analytic expression of geometric discords in the states is worked out. Some concrete discussions and analyses on the captured geometric discords are made so that their distinct features are exposed. It is found that, the more averagely the two bi-qubit product states are mixed, the bigger geometric discord the mixed state owns. Moreover, the monotonic relationships of geometric discord with different parameters are revealed.
