A full length amphioxus cDNA, encoding a novel phosducin-like protein （Amphi-PhLP）, was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open read...A full length amphioxus cDNA, encoding a novel phosducin-like protein （Amphi-PhLP）, was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame （ORF） of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind grit and testis, hepatic caecum, gill, endostyle, and epipbaryngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary （CHO） cells transfected with the expression plasmid pEGFP-NI/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.展开更多
A new concept of rare axis based on statistical facts is proposed, and an evaluation algorithm is designed thereafter. For the nested regular expressions containing rare axes, the proposed algorithm can reduce its eva...A new concept of rare axis based on statistical facts is proposed, and an evaluation algorithm is designed thereafter. For the nested regular expressions containing rare axes, the proposed algorithm can reduce its evaluation complexity from polynomial time to nearly linear time. The distributed technique is also employed to construct the navigation axis indexes for resource description framework(RDF) graph data. Experiment results in Drug Bank and Bio GRID show that this method can improve the query efficiency significantly while ensuring the accuracy and meet the query requirements on Web-scale RDF graph data.展开更多
To obtain the helper plasmids for a reverse genetics system of rabies virus, the cDNAs of the complete open reading frames of the N, P, G, and L genes of rabies street virus stain HN10 were each cloned into expression...To obtain the helper plasmids for a reverse genetics system of rabies virus, the cDNAs of the complete open reading frames of the N, P, G, and L genes of rabies street virus stain HN10 were each cloned into expression vector pVAX1, These four plasmids were identified by restriction enzyme digestion and gene sequencing. The plasmid encoding the N protein was selected to determine the expression effect of these plasmids in NA cells. The results showed that the helper plasmids for a reverse genetics system of rabies street virus strain HN10 had been successfully constructed.展开更多
基金Supported by the Pilot Projects of Knowledge Innovation Project of Chinese Academy of Sciences (No. KZCX2-YW-211-03 and MGE2008KG06)
文摘A full length amphioxus cDNA, encoding a novel phosducin-like protein （Amphi-PhLP）, was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame （ORF） of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind grit and testis, hepatic caecum, gill, endostyle, and epipbaryngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary （CHO） cells transfected with the expression plasmid pEGFP-NI/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri.
基金Supported by the National Natural Science Foundation of China(No.61373035 and No.61100049)National High Technology Research and Development Program of China("863"Program,No.2013AA013204)+1 种基金Fundamental Research Funds for the Central Universities(No.3122014C018 and 3122015C022)Scientific Research Funds Supported by Civil Aviation University of China(No.09QD02X)
文摘A new concept of rare axis based on statistical facts is proposed, and an evaluation algorithm is designed thereafter. For the nested regular expressions containing rare axes, the proposed algorithm can reduce its evaluation complexity from polynomial time to nearly linear time. The distributed technique is also employed to construct the navigation axis indexes for resource description framework(RDF) graph data. Experiment results in Drug Bank and Bio GRID show that this method can improve the query efficiency significantly while ensuring the accuracy and meet the query requirements on Web-scale RDF graph data.
基金National High Technology Research and Development Program of China (2006AA02Z110, 2007AA02Z402)Major Program of the National Natural Science Foundation of China (30630049)
文摘To obtain the helper plasmids for a reverse genetics system of rabies virus, the cDNAs of the complete open reading frames of the N, P, G, and L genes of rabies street virus stain HN10 were each cloned into expression vector pVAX1, These four plasmids were identified by restriction enzyme digestion and gene sequencing. The plasmid encoding the N protein was selected to determine the expression effect of these plasmids in NA cells. The results showed that the helper plasmids for a reverse genetics system of rabies street virus strain HN10 had been successfully constructed.
