加筋板在轴压载荷下的承载能力受初始几何缺陷的影响,故而重构含几何缺陷的有限元模型以及分析缺陷对加筋板前屈曲/后屈曲特性影响均是值得深入研究的问题。为此,采用GMNIA(Geometrically and Material Nonlinear Analysis with Imperfe...加筋板在轴压载荷下的承载能力受初始几何缺陷的影响,故而重构含几何缺陷的有限元模型以及分析缺陷对加筋板前屈曲/后屈曲特性影响均是值得深入研究的问题。为此,采用GMNIA(Geometrically and Material Nonlinear Analysis with Imperfections)方法分析了特征值模态缺陷的幅值、公式表达几何缺陷的形状对加筋板的承载能力的影响,并将承载能力进行了试验校验。研究结果表明:初始几何缺陷通过降低加筋板初始刚度对轴压载荷下加筋板的前屈曲行为有一定的影响,而初始缺陷的显著影响表现在后屈曲段,明显地表现为后屈曲路径和极限承载能力的差异。引入缺陷的加筋板轴压承载能力的有限元计算值与试验值相对误差均小于4.0%。因此,引入特征值模态缺陷和公式表达缺陷的GMNIA分析方法可用于加筋板轴压承载能力的工程设计。展开更多
AIM:To investigate the relationship between blood riboflavin levels and riboflavin transporter 2(RFT2) gene expression in gastric carcinoma(GC) development.METHODS:High-performance liquid chromatography was used to de...AIM:To investigate the relationship between blood riboflavin levels and riboflavin transporter 2(RFT2) gene expression in gastric carcinoma(GC) development.METHODS:High-performance liquid chromatography was used to detect blood riboflavin levels in patients with GC.Real-time fluorogenic quantitative polymerase chain reaction and immunohistochemistry were used to analyze the expression of RFT2 mRNA and protein in samples from 60 GC patients consisting of both tumor and normal tissue.RESULTS:A significant decrease in the RFT2 mRNA levels was detected in GC samples compared with those in the normal mucous membrane(0.398 ± 0.149 vs 1.479 ± 0.587;P = 0.040).Tumors exhibited low RFT2 protein expression(75%,16.7%,8.3% and 0% for no RFT2 staining,weak staining,medium staining and strong staining,respectively),which was significantly lower than that in the normal mucous membrane(10%,16.7%,26.7% and 46.7% for no RFT2 staining,weak staining,medium staining and strong staining,respectively;P < 0.05).Tumors with low RFT2 expression were significantly associated with tumor stage and histological grade.Moreover,a significantly decrease in Uyghur patients was observed compared with Han patients.However,other parameters-gender,tumor location and lymph node metastasis-showed no significant relationship with RFT2 expression.Blood riboflavin levels were reverse correlated with development of GC(1.2000 ± 0.97 569 ng/mL in high tumor stage patients vs 2.5980 ± 1.31 129 ng/mL in low tumor stage patients;P < 0.05).A positive correlation of plasma riboflavin levels with defective expression of RFT2 protein was found in GC patients(2 = 2.619;P = 0.019).CONCLUSION:Defective expression of RFT2 is associated with the development of GC and this may represent a mechanism underlying the decreased plasma riboflavin levels in GC.展开更多
A pair of primers was designed according to the reported conserved sequence of the defective in anther dehiscencel （DAD1） gene ofArabidopsis thaliana and Brassica rapa. A 558 bp long fragment was amplified from geno...A pair of primers was designed according to the reported conserved sequence of the defective in anther dehiscencel （DAD1） gene ofArabidopsis thaliana and Brassica rapa. A 558 bp long fragment was amplified from genomic DNA of Chinese kale, showing more than 88% identity with the known DAD1 nucleotide sequence and no intron. The reverse of the amplified fragment was ligated to the downstream of the CaMV35S promoter in the plant expression vector pBIl21. Antisense expression vector pBII21-DAD1F was constructed with DAD1 fragment of Chinese kale, and was transferred into Agrobacterium tumefaciens, which will be used in the transformation to create male sterile materials of Chinese kale.展开更多
文摘加筋板在轴压载荷下的承载能力受初始几何缺陷的影响,故而重构含几何缺陷的有限元模型以及分析缺陷对加筋板前屈曲/后屈曲特性影响均是值得深入研究的问题。为此,采用GMNIA(Geometrically and Material Nonlinear Analysis with Imperfections)方法分析了特征值模态缺陷的幅值、公式表达几何缺陷的形状对加筋板的承载能力的影响,并将承载能力进行了试验校验。研究结果表明:初始几何缺陷通过降低加筋板初始刚度对轴压载荷下加筋板的前屈曲行为有一定的影响,而初始缺陷的显著影响表现在后屈曲段,明显地表现为后屈曲路径和极限承载能力的差异。引入缺陷的加筋板轴压承载能力的有限元计算值与试验值相对误差均小于4.0%。因此,引入特征值模态缺陷和公式表达缺陷的GMNIA分析方法可用于加筋板轴压承载能力的工程设计。
基金Supported by The National Natural Science Foundation of China,No.81160459
文摘AIM:To investigate the relationship between blood riboflavin levels and riboflavin transporter 2(RFT2) gene expression in gastric carcinoma(GC) development.METHODS:High-performance liquid chromatography was used to detect blood riboflavin levels in patients with GC.Real-time fluorogenic quantitative polymerase chain reaction and immunohistochemistry were used to analyze the expression of RFT2 mRNA and protein in samples from 60 GC patients consisting of both tumor and normal tissue.RESULTS:A significant decrease in the RFT2 mRNA levels was detected in GC samples compared with those in the normal mucous membrane(0.398 ± 0.149 vs 1.479 ± 0.587;P = 0.040).Tumors exhibited low RFT2 protein expression(75%,16.7%,8.3% and 0% for no RFT2 staining,weak staining,medium staining and strong staining,respectively),which was significantly lower than that in the normal mucous membrane(10%,16.7%,26.7% and 46.7% for no RFT2 staining,weak staining,medium staining and strong staining,respectively;P < 0.05).Tumors with low RFT2 expression were significantly associated with tumor stage and histological grade.Moreover,a significantly decrease in Uyghur patients was observed compared with Han patients.However,other parameters-gender,tumor location and lymph node metastasis-showed no significant relationship with RFT2 expression.Blood riboflavin levels were reverse correlated with development of GC(1.2000 ± 0.97 569 ng/mL in high tumor stage patients vs 2.5980 ± 1.31 129 ng/mL in low tumor stage patients;P < 0.05).A positive correlation of plasma riboflavin levels with defective expression of RFT2 protein was found in GC patients(2 = 2.619;P = 0.019).CONCLUSION:Defective expression of RFT2 is associated with the development of GC and this may represent a mechanism underlying the decreased plasma riboflavin levels in GC.
文摘A pair of primers was designed according to the reported conserved sequence of the defective in anther dehiscencel （DAD1） gene ofArabidopsis thaliana and Brassica rapa. A 558 bp long fragment was amplified from genomic DNA of Chinese kale, showing more than 88% identity with the known DAD1 nucleotide sequence and no intron. The reverse of the amplified fragment was ligated to the downstream of the CaMV35S promoter in the plant expression vector pBIl21. Antisense expression vector pBII21-DAD1F was constructed with DAD1 fragment of Chinese kale, and was transferred into Agrobacterium tumefaciens, which will be used in the transformation to create male sterile materials of Chinese kale.
