The performance of wxtaraguiatory senescence-inhibition gene PSAG12-IPT in nee has been investigated in the study.422 tranagenic plants from 134 independent resistant calli were obtained from 4 rice varieties through ...The performance of wxtaraguiatory senescence-inhibition gene PSAG12-IPT in nee has been investigated in the study.422 tranagenic plants from 134 independent resistant calli were obtained from 4 rice varieties through Agrobacterium-mediated transformation.Among them,233 were positive PSAGIZ^IPT tranagenic plants identified by GUS histochemical assay and PCR analysis.Southern analysis showed the transgene was randomly integrated into rice genome,of which 42.29% was single copy.Investigations on photosynthesis function and agronomic characters of Rt generation showed that chlorophy Ⅱ content and photosynthesis rate of flag leaves in tranagenic plants,were 41.23% and 60.24% higher than the control wild-type rice,respectively.The growth duration and plant height of the tranagenic plants were similar to the control.Variations of other characters were dependent on the varieties.For the variety Millin with significant aging phenomenon in China,its total grains per hill,its seed setting rate and 1000-grain weight were increased by 40.44%,8.05% and 8.32% respectively.The results indicated that after leaf senescence of varieties liable to age was delayed,the seed setting rate and the filling degree of seeds were improved,which finally resulted in significantly increased seed yield and taomaas per hill.The new variety Wuyujing 2 without serious aging problem,was also increased in the panicles per hill,the total grains per hill,the seed yield per hill and biomass in different degrees.展开更多
文摘The performance of wxtaraguiatory senescence-inhibition gene PSAG12-IPT in nee has been investigated in the study.422 tranagenic plants from 134 independent resistant calli were obtained from 4 rice varieties through Agrobacterium-mediated transformation.Among them,233 were positive PSAGIZ^IPT tranagenic plants identified by GUS histochemical assay and PCR analysis.Southern analysis showed the transgene was randomly integrated into rice genome,of which 42.29% was single copy.Investigations on photosynthesis function and agronomic characters of Rt generation showed that chlorophy Ⅱ content and photosynthesis rate of flag leaves in tranagenic plants,were 41.23% and 60.24% higher than the control wild-type rice,respectively.The growth duration and plant height of the tranagenic plants were similar to the control.Variations of other characters were dependent on the varieties.For the variety Millin with significant aging phenomenon in China,its total grains per hill,its seed setting rate and 1000-grain weight were increased by 40.44%,8.05% and 8.32% respectively.The results indicated that after leaf senescence of varieties liable to age was delayed,the seed setting rate and the filling degree of seeds were improved,which finally resulted in significantly increased seed yield and taomaas per hill.The new variety Wuyujing 2 without serious aging problem,was also increased in the panicles per hill,the total grains per hill,the seed yield per hill and biomass in different degrees.
