目的以海藻海蒿子为原料制备褐藻糖胶,并对不同的提取、分离方法进行比较,对多糖的组成和结构进行初步分析。方法海蒿子脱脂干粉分别经水提取和酸提取以获得粗多糖,粗多糖分别经乙醇沉淀法和CaCl2沉淀法进行纯化。选取多糖组分F4用Q-Sep...目的以海藻海蒿子为原料制备褐藻糖胶,并对不同的提取、分离方法进行比较,对多糖的组成和结构进行初步分析。方法海蒿子脱脂干粉分别经水提取和酸提取以获得粗多糖,粗多糖分别经乙醇沉淀法和CaCl2沉淀法进行纯化。选取多糖组分F4用Q-Sepharose Fast Flow和Sephadex G-200凝胶柱进行分级分离,高效凝胶渗透色谱法(HPGPC)鉴定纯度及相对分子质量,气相色谱分析多糖的中性单糖组成。结果F4经分级分离得到3个级分:P1、P2和P3。P1、P2和P3均为均一组分,相对分子质量分别为:494400、61500和167600;P1由岩藻糖、木糖、甘露糖、葡萄糖和半乳糖构成,摩尔百分比43.4∶33.3∶6.2∶4.3∶12.8;P2由岩藻糖、木糖、甘露糖和半乳糖构成,摩尔百分比44.4∶15.1∶23.8∶16.7;P3由岩藻糖、木糖和半乳糖构成,摩尔百分比68.9∶3.7∶27.4。结论P1、P2和P3的单糖含量均以岩藻糖为主,但其他单糖组成有较大差别。展开更多
该实验优化了裙带菜褐藻糖胶的提取工艺,对粗多糖进行了分离纯化,并研究了各组分的单糖组成和硫酸根含量。利用响应面优化法,依据二次回归分析确定裙带菜褐藻糖胶的最佳提取工艺为:提取温度76℃,提取时间2.5h,料液比1︰6.5。在此条件下...该实验优化了裙带菜褐藻糖胶的提取工艺,对粗多糖进行了分离纯化,并研究了各组分的单糖组成和硫酸根含量。利用响应面优化法,依据二次回归分析确定裙带菜褐藻糖胶的最佳提取工艺为:提取温度76℃,提取时间2.5h,料液比1︰6.5。在此条件下提取2次,粗多糖的提取率可达到7.53%。提取的粗多糖用DEAE-Sepharose Fast Flow离子交换层析分离纯化,共得到5个峰,干燥后得到5个组分。用硫酸钡比浊法测定5个组分的硫酸根含量分别为8.3%、19.2%、30.1%、38.4%和33.0%。糖腈乙酸酯衍生物气相色谱测得各组分的单糖组成分别为:组分1主要含有甘露糖和半乳糖;组分2主要含有鼠李糖,岩藻糖,木糖,甘露糖,葡萄糖和半乳糖;组分3主要含有鼠李糖,岩藻糖和甘露糖;组分4主要含有岩藻糖和半乳糖;组分5主要含有岩藻糖,甘露糖和半乳糖。展开更多
Silk fibroin (SF)/fucoidan blend films with different blend ratios were prepared by mixing aqueous solutions of SF and fucoidan.The structural characteristics and thermal properties were examined through Fourier trans...Silk fibroin (SF)/fucoidan blend films with different blend ratios were prepared by mixing aqueous solutions of SF and fucoidan.The structural characteristics and thermal properties were examined through Fourier transform infrared (FTIR) spectroscopy,X-ray diffractometry (XRD),thermogravimetry,and differential scanning calorimetry (DSC).The conformation of SF in blend films was revealed to be a β-sheet structure,according to the results of FTIR and XRD spectra.This result was also confirmed through DSC thermograms,the exotherm at around 230℃,that was attributed to the random coil conformation of SF,was not observed in blend films.These results demonstrated that the conformation change of SF occurred from a random coil to a β-sheet structure due to the formation of intermolecular hydrogen bonds between fucoidan and SF in the blend films.The crystalline SF films showed higher thermal stability than the amorphous SF film.展开更多
文摘目的以海藻海蒿子为原料制备褐藻糖胶,并对不同的提取、分离方法进行比较,对多糖的组成和结构进行初步分析。方法海蒿子脱脂干粉分别经水提取和酸提取以获得粗多糖,粗多糖分别经乙醇沉淀法和CaCl2沉淀法进行纯化。选取多糖组分F4用Q-Sepharose Fast Flow和Sephadex G-200凝胶柱进行分级分离,高效凝胶渗透色谱法(HPGPC)鉴定纯度及相对分子质量,气相色谱分析多糖的中性单糖组成。结果F4经分级分离得到3个级分:P1、P2和P3。P1、P2和P3均为均一组分,相对分子质量分别为:494400、61500和167600;P1由岩藻糖、木糖、甘露糖、葡萄糖和半乳糖构成,摩尔百分比43.4∶33.3∶6.2∶4.3∶12.8;P2由岩藻糖、木糖、甘露糖和半乳糖构成,摩尔百分比44.4∶15.1∶23.8∶16.7;P3由岩藻糖、木糖和半乳糖构成,摩尔百分比68.9∶3.7∶27.4。结论P1、P2和P3的单糖含量均以岩藻糖为主,但其他单糖组成有较大差别。
文摘该实验优化了裙带菜褐藻糖胶的提取工艺,对粗多糖进行了分离纯化,并研究了各组分的单糖组成和硫酸根含量。利用响应面优化法,依据二次回归分析确定裙带菜褐藻糖胶的最佳提取工艺为:提取温度76℃,提取时间2.5h,料液比1︰6.5。在此条件下提取2次,粗多糖的提取率可达到7.53%。提取的粗多糖用DEAE-Sepharose Fast Flow离子交换层析分离纯化,共得到5个峰,干燥后得到5个组分。用硫酸钡比浊法测定5个组分的硫酸根含量分别为8.3%、19.2%、30.1%、38.4%和33.0%。糖腈乙酸酯衍生物气相色谱测得各组分的单糖组成分别为:组分1主要含有甘露糖和半乳糖;组分2主要含有鼠李糖,岩藻糖,木糖,甘露糖,葡萄糖和半乳糖;组分3主要含有鼠李糖,岩藻糖和甘露糖;组分4主要含有岩藻糖和半乳糖;组分5主要含有岩藻糖,甘露糖和半乳糖。
文摘Silk fibroin (SF)/fucoidan blend films with different blend ratios were prepared by mixing aqueous solutions of SF and fucoidan.The structural characteristics and thermal properties were examined through Fourier transform infrared (FTIR) spectroscopy,X-ray diffractometry (XRD),thermogravimetry,and differential scanning calorimetry (DSC).The conformation of SF in blend films was revealed to be a β-sheet structure,according to the results of FTIR and XRD spectra.This result was also confirmed through DSC thermograms,the exotherm at around 230℃,that was attributed to the random coil conformation of SF,was not observed in blend films.These results demonstrated that the conformation change of SF occurred from a random coil to a β-sheet structure due to the formation of intermolecular hydrogen bonds between fucoidan and SF in the blend films.The crystalline SF films showed higher thermal stability than the amorphous SF film.
