In this study, identification and rDNA-ITS sequence analysis of an anthracnose pathogen on Dracaena fragrans were carried out. [Method] D. fra-grans leaves with lesions were used as experimental materials to isolate a...In this study, identification and rDNA-ITS sequence analysis of an anthracnose pathogen on Dracaena fragrans were carried out. [Method] D. fra-grans leaves with lesions were used as experimental materials to isolate anthrac-nose pathogen. Morphological observation, rDNA-ITS amplification and sequence analysis were performed to identify the pathogen strain. [Result] Caonidia of the iso-lated anthracnose pathogen were straight or curved, el iptic to crescent, with 2-5 oil droplets, 7.5-20 × 4.5-5 μm. According to molecular phylogenetic analysis, the iso-lated pathogen strain was identified as a new species, which was named Col-letotrichum dracaena-fragrantis sp. nov. [Conclusion] This study provided theoretical basis for the prevention and control of anthracnose.展开更多
Tensile failure(spalling or slabbing)often occurs on the sidewall of deep tunnel,which is closely related to the coupled stress state of deep rock mass under high pre-static load and dynamic disturbance.To reveal the ...Tensile failure(spalling or slabbing)often occurs on the sidewall of deep tunnel,which is closely related to the coupled stress state of deep rock mass under high pre-static load and dynamic disturbance.To reveal the mechanism of rock tensile failure caused by this coupled stress mode,the Brazilian disc tests were carried on red sandstone under high pre-static load induced by dynamic disturbance.Based on the pure static tensile fracture load of red sandstone specimen,two static load levels(80%and 90%of the pure static tensile fracture load)were selected as the initial high pre-static loading state,and then the dynamic disturbance load was applied until the rock specimen was destroyed.The dynamic disturbance loading mode adopted a sinusoidal wave(sine-wave)load,and the loading wave amplitude was 20%and 10%of the pure static tensile fracture load,respectively.The dynamic disturbance frequencies were set to 1,10,20,30,40,and 50 Hz.The results show that the tensile failure strength and peak displacement of red sandstone specimens under coupled load actions are lower than those under pure static tensile load,and both parameters decrease significantly with the increase of dynamic disturbance frequency.With the increase of dynamic disturbance frequency,the decrease range of tensile strength of red sandstone increased from 3.3%to 9.4%when the pre-static load level is 80%.While when the pre-static load level is 90%,the decrease range will increase from 7.4%to 11.6%.This weakening effect of tensile strength shows that the deep surrounding rock is more likely to fail under the coupled load actions of pre-static load and dynamic disturbance.In this tensile failure mechanism of the deep surrounding rock,the stress environment of deep sidewall rock determines that the failure mode of rock is a tensile failure,the pre-static load level dominates the tensile failure strength of surrounding rock,and dynamic disturbance promotes the strength-weakening effect and affects the weakening range.展开更多
To reveal stress distribution and crack propagation of Brazilian discs under impact loads, dynamic tests were conducted with SHPB （split Hopkinson pressure bar） device. Stress states of specimens were monitored with...To reveal stress distribution and crack propagation of Brazilian discs under impact loads, dynamic tests were conducted with SHPB （split Hopkinson pressure bar） device. Stress states of specimens were monitored with strain gauges on specimen surface and SHPB bars. The failure process of specimen was recorded by ultra speed camera FASTCAM SAI.1 （675 000 fps）. Stress histories from strain gauges offer comprehensive information to evaluate the stress equilibrium of specimen in time and space. When a slowly rising load （with loading rates less than 1 200 N/s for d 50 mm bar） is applied, there is usually good stress equilibrium in specimen. The stress distribution after equilibrium is similar to its static counterpart. And the first crack initiates at the disc center and propagates along the load direction. But with the front of incident wave becoming steep, it is hard for specimens to get to stress equilibrium. The first crack may appear anywhere on the specimen together with multiple randomly distributed secondary cracks. For a valid dynamic Brazil test with stress equilibrium, the specimen will break into two halves neatly. While for tests with stress disequilibrium, missing strap may be found when broken halves of specimens are put together. For those specimens broken up neatly at center but having missing wedges at the loading areas, it is usually subjected to local buckling from SHPB bars.展开更多
Results of investigations into the CO2 content in tree disc rings by the method proposed here have shown that a considerable part of CO2 generated under cell respiration is found in tree stems. Besides, annual CO2 dis...Results of investigations into the CO2 content in tree disc rings by the method proposed here have shown that a considerable part of CO2 generated under cell respiration is found in tree stems. Besides, annual CO2 distribution in tree rings exhibits a well-defined cyclicity. Results obtained from investigations into long-term CO2 and H2O variations in tree discs performed by different methods are presented. Wavelet and spectral analyses of the relationship between CO2 and H2O variations in a Siberian stone pine disc and meteorological parameters were made. The CO2 annual distributions of seven spruce tree discs were examined by the Caterpiller-SSA method. Wood samples of Siberian stone pine and spruce trees were taken from the same site in Tomsk region （Siberia, Russia） that characterized by an optimum growth habitat. Conclusions are made regarding the response of the annual CO2 and H2O conifer disc tree ring distributions to different climatic factors.展开更多
A method that uses finite element analysis to determine the non-singular stress （T-stress） at a crack tip is proposed in this study. T-stress includes two components： the Tx-stress parallel to the tangent of the cr...A method that uses finite element analysis to determine the non-singular stress （T-stress） at a crack tip is proposed in this study. T-stress includes two components： the Tx-stress parallel to the tangent of the crack at its tip and the Ty-stress perpendicular to this tangent. The effects of contact and friction on both the Tx- and Ty-stresses on the crack flanks are considered in the method. Because the method uses a single standard elastic finite element analysis derived directly from the equation of the stress fields around the crack tip and does not require any assumptions or simplification, it can be used to determine the T-stress for any given geometry and loading condition. Theoretical results are used to calibrate the results, which exhibited good agreement and to discuss the T-stress computational methodology. Furthermore, the Tx- and Ty-stresses in center-cracked Brazilian disc （CCBD） specimens subjected to diametrical or partially distributed compression were numerically computed, and the effects of contact and friction on the Tx- and Ty-stresses are discussed.展开更多
文摘In this study, identification and rDNA-ITS sequence analysis of an anthracnose pathogen on Dracaena fragrans were carried out. [Method] D. fra-grans leaves with lesions were used as experimental materials to isolate anthrac-nose pathogen. Morphological observation, rDNA-ITS amplification and sequence analysis were performed to identify the pathogen strain. [Result] Caonidia of the iso-lated anthracnose pathogen were straight or curved, el iptic to crescent, with 2-5 oil droplets, 7.5-20 × 4.5-5 μm. According to molecular phylogenetic analysis, the iso-lated pathogen strain was identified as a new species, which was named Col-letotrichum dracaena-fragrantis sp. nov. [Conclusion] This study provided theoretical basis for the prevention and control of anthracnose.
基金Projects(42077244,41877272,41472269)supported by the National Natural Science Foundation of ChinaProject(2242020R10023)supported by the Fundamental Research Funds for the Central Universities of Southeast University,China。
文摘Tensile failure(spalling or slabbing)often occurs on the sidewall of deep tunnel,which is closely related to the coupled stress state of deep rock mass under high pre-static load and dynamic disturbance.To reveal the mechanism of rock tensile failure caused by this coupled stress mode,the Brazilian disc tests were carried on red sandstone under high pre-static load induced by dynamic disturbance.Based on the pure static tensile fracture load of red sandstone specimen,two static load levels(80%and 90%of the pure static tensile fracture load)were selected as the initial high pre-static loading state,and then the dynamic disturbance load was applied until the rock specimen was destroyed.The dynamic disturbance loading mode adopted a sinusoidal wave(sine-wave)load,and the loading wave amplitude was 20%and 10%of the pure static tensile fracture load,respectively.The dynamic disturbance frequencies were set to 1,10,20,30,40,and 50 Hz.The results show that the tensile failure strength and peak displacement of red sandstone specimens under coupled load actions are lower than those under pure static tensile load,and both parameters decrease significantly with the increase of dynamic disturbance frequency.With the increase of dynamic disturbance frequency,the decrease range of tensile strength of red sandstone increased from 3.3%to 9.4%when the pre-static load level is 80%.While when the pre-static load level is 90%,the decrease range will increase from 7.4%to 11.6%.This weakening effect of tensile strength shows that the deep surrounding rock is more likely to fail under the coupled load actions of pre-static load and dynamic disturbance.In this tensile failure mechanism of the deep surrounding rock,the stress environment of deep sidewall rock determines that the failure mode of rock is a tensile failure,the pre-static load level dominates the tensile failure strength of surrounding rock,and dynamic disturbance promotes the strength-weakening effect and affects the weakening range.
基金Projects(50904079, 51274254, 50934006) supported by the National Natural Science Foundation of ChinaProject(2010CB732004) supported by the National Basic Research Program of ChinaProject(NCET-11-0528) supported by Program for New Century Excellent Talents in University of China
文摘To reveal stress distribution and crack propagation of Brazilian discs under impact loads, dynamic tests were conducted with SHPB （split Hopkinson pressure bar） device. Stress states of specimens were monitored with strain gauges on specimen surface and SHPB bars. The failure process of specimen was recorded by ultra speed camera FASTCAM SAI.1 （675 000 fps）. Stress histories from strain gauges offer comprehensive information to evaluate the stress equilibrium of specimen in time and space. When a slowly rising load （with loading rates less than 1 200 N/s for d 50 mm bar） is applied, there is usually good stress equilibrium in specimen. The stress distribution after equilibrium is similar to its static counterpart. And the first crack initiates at the disc center and propagates along the load direction. But with the front of incident wave becoming steep, it is hard for specimens to get to stress equilibrium. The first crack may appear anywhere on the specimen together with multiple randomly distributed secondary cracks. For a valid dynamic Brazil test with stress equilibrium, the specimen will break into two halves neatly. While for tests with stress disequilibrium, missing strap may be found when broken halves of specimens are put together. For those specimens broken up neatly at center but having missing wedges at the loading areas, it is usually subjected to local buckling from SHPB bars.
文摘Results of investigations into the CO2 content in tree disc rings by the method proposed here have shown that a considerable part of CO2 generated under cell respiration is found in tree stems. Besides, annual CO2 distribution in tree rings exhibits a well-defined cyclicity. Results obtained from investigations into long-term CO2 and H2O variations in tree discs performed by different methods are presented. Wavelet and spectral analyses of the relationship between CO2 and H2O variations in a Siberian stone pine disc and meteorological parameters were made. The CO2 annual distributions of seven spruce tree discs were examined by the Caterpiller-SSA method. Wood samples of Siberian stone pine and spruce trees were taken from the same site in Tomsk region （Siberia, Russia） that characterized by an optimum growth habitat. Conclusions are made regarding the response of the annual CO2 and H2O conifer disc tree ring distributions to different climatic factors.
基金supported by the National Basic Research Program of China(Grant No.2014CB047100)the National Natural Science Foundation of China(Grant Nos.51474046,U1562103)the Opening Fund of State Key Laboratory of Geohazard Prevention and Geoenvironment Protection(Chengdu University of Technology)(Grant No.SKLGP2014K017)
文摘A method that uses finite element analysis to determine the non-singular stress （T-stress） at a crack tip is proposed in this study. T-stress includes two components： the Tx-stress parallel to the tangent of the crack at its tip and the Ty-stress perpendicular to this tangent. The effects of contact and friction on both the Tx- and Ty-stresses on the crack flanks are considered in the method. Because the method uses a single standard elastic finite element analysis derived directly from the equation of the stress fields around the crack tip and does not require any assumptions or simplification, it can be used to determine the T-stress for any given geometry and loading condition. Theoretical results are used to calibrate the results, which exhibited good agreement and to discuss the T-stress computational methodology. Furthermore, the Tx- and Ty-stresses in center-cracked Brazilian disc （CCBD） specimens subjected to diametrical or partially distributed compression were numerically computed, and the effects of contact and friction on the Tx- and Ty-stresses are discussed.
