106 aeeessions of Tibetan wild barley, including 50 accessions of the two-rowed wiht barley Hordeum vulgare ssp. sopntaneum （HS）, 27 accessions of the six-rowed bottle-shaped wild barley H. lagunculiforme （HL） and...106 aeeessions of Tibetan wild barley, including 50 accessions of the two-rowed wiht barley Hordeum vulgare ssp. sopntaneum （HS）, 27 accessions of the six-rowed bottle-shaped wild barley H. lagunculiforme （HL） and 29 accessions of the six-rowed wiht barley H. agriocrithon （HA） that separately represent different agrigeographical regions of Tibet, were used to study the genetic diversity and genetie differentiation using SSR markers selected from seven barley linkage groups. 229 allelic variants were identified with an average of 7.6 alleles/locus. The average of total number of alleles per locus in HA （6.4） is much higher than that in HS （3.9） and HL （3.4）. The genetie diversity and its standard deviation among the three subspecies were in the order of HS〉HL〉HA. Very significant genetic differentiation was observed among the three subspecies of wild barley. Comparisons of the results fiom this and previous studies showed a strong Oriental-Occidental differentiation of barley, and that Shannan region of Tibet might be the center of origin of the Tibetan two-rowed wild barley, thus supporting not only the hypothesis of a mono-phyletie origin of cultivated barley but also the proposition that the Tibetan two-rowed wild barley as ultimate progenitor of Chinese cultivated barley.展开更多
Z961215 大麦核糖体失活蛋白基因的克隆及序列分析/吴志浩,朱祯…(中科院遗传所)//农业生物技术学报.-1995,3(4).-64～70以大麦基因组 DNA 为模板,通过合成5′-端及3′-端的一对特异引物,利用多聚酶链式反应(PCR)扩增出了大麦核糖体失...Z961215 大麦核糖体失活蛋白基因的克隆及序列分析/吴志浩,朱祯…(中科院遗传所)//农业生物技术学报.-1995,3(4).-64～70以大麦基因组 DNA 为模板,通过合成5′-端及3′-端的一对特异引物,利用多聚酶链式反应(PCR)扩增出了大麦核糖体失活蛋白编码序列,随后将其克隆到 E.coli 质粒上。序列分析表明,大麦核糖体失活蛋白基因由846个核苷酸组成,编码由281个氨基酸残基组成的多肽。与发表序列相比较,核苷酸序列及推导的氨基酸序列的同源性分别为93.1％和92％。(图5参9)Z961216 西藏裸大麦主要性状的遗传参数研究/王建林,胡书银…(西藏农牧学院农学系)//遗传.-1996,18(2).展开更多
基金Supported by the National Nature Science Foundation of China(NSFC)(No.30630047)Supporting Plan of Science and Technology in China(No.2006BAD02B04-3)Special Fund for Public Industry in China(No.N20070331-01)
文摘106 aeeessions of Tibetan wild barley, including 50 accessions of the two-rowed wiht barley Hordeum vulgare ssp. sopntaneum （HS）, 27 accessions of the six-rowed bottle-shaped wild barley H. lagunculiforme （HL） and 29 accessions of the six-rowed wiht barley H. agriocrithon （HA） that separately represent different agrigeographical regions of Tibet, were used to study the genetic diversity and genetie differentiation using SSR markers selected from seven barley linkage groups. 229 allelic variants were identified with an average of 7.6 alleles/locus. The average of total number of alleles per locus in HA （6.4） is much higher than that in HS （3.9） and HL （3.4）. The genetie diversity and its standard deviation among the three subspecies were in the order of HS〉HL〉HA. Very significant genetic differentiation was observed among the three subspecies of wild barley. Comparisons of the results fiom this and previous studies showed a strong Oriental-Occidental differentiation of barley, and that Shannan region of Tibet might be the center of origin of the Tibetan two-rowed wild barley, thus supporting not only the hypothesis of a mono-phyletie origin of cultivated barley but also the proposition that the Tibetan two-rowed wild barley as ultimate progenitor of Chinese cultivated barley.
